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Proprotein Convertase 2 (PCSK2) (NM_001201529) Human Tagged ORF Clone
CAT＃: RG233003
- TrueORF®
PCSK2 (tGFP-tagged) - Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2
ORF Plasmid: DDK
"NM_001201529" in other vectors (2)
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CNY 5,040.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NEC-2; NEC 2; NEC2; PC2; SPC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233003 representing NM_001201529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGTGGTTGTGTCTCCCAGTGGAAGGCGGCCGCCGGGTTCCTCTTCTGTGTCATGGTTTTTGCAT CTGCTGAGCGACCGGTCTTCACGAATCATTTTCTTGTGGAGTTGCATAAAGGGGGAGAGGACAAAGCTCG CCAAGTTGCAGCAGAACACGGCTTTGGAGTCCGAAAGGTAAAGATGGCTTTGCAGCAGGAAGGATTTGAC CGAAAAAAGCGAGGTTACAGAGACATCAATGAGATCGACATCAACATGAACGATCCTCTTTTTACAAAGC AGTGGTATCTGATCAATACTGGGCAAGCTGATGGCACTCCTGGCCTTGATTTGAATGTGGCTGAAGCCTG GGAGCTGGGATACACAGGGAAAGGTGTTACCATTGGAATTATGGATGATGGGATTGACTATCTCCACCCG GACCTGGCCTCCAACTATAATGCCGAAGCAAGTTACGACTTCAGCAGCAACGACCCCTATCCTTACCCTC GGTACACAGATGACTGGTTTAACAGCCACGGGACCCGATGTGCAGGAGAAGTTTCTGCTGCCGCCAACAA CAATATCTGTGGAGTTGGAGTAGCATACAACTCCAAGGTTGCAGGCATCCGGATGCTGGACCAGCCATTC ATGACAGACATCATCGAGGCCTCCTCCATCAGTCATATGCCACAGCTGATTGACATCTACAGCGCCAGCT GGGGCCCCACAGACAACGGCAAGACAGTGGATGGGCCCCGGGAGCTCACGCTGCAGGCCATGGCCGATGG CGTGAACAAGGGCCGCGGCGGCAAAGGCAGCATCTACGTGTGGGCCTCCGGGGACGGCGGCAGCTATGAC GACTGCAACTGCGACGGCTACGCCTCCAGCATGTGGACCATCTCCATCAACTCAGCCATCAACGACGGCA GGACTGCCCTGTACGACGAGAGCTGCTCTTCCACCTTGGCTTCCACCTTCAGCAACGGGAGGAAAAGGAA CCCCGAGGCCGGTGTGGCAACCACAGATTTGTACGGCAACTGCACTCTGAGGCATTCTGGGACATCTGCA GCTGCCCCCGAGGCAGCTGGTGTGTTTGCACTGGCTCTGGAGGCTAACCTGGGTCTGACCTGGCGGGACA TGCAGCATCTGACTGTGCTCACCTCCAAACGGAACCAGCTTCACGACGAGGTCCATCAGTGGCGGCGCAA TGGGGTCGGCCTGGAATTTAATCACCTCTTTGGCTACGGGGTCCTTGATGCAGGTGCCATGGTGAAAATG GCTAAAGACTGGAAAACCGTGCCTGAGAGATTCCACTGTGTGGGAGGCTCCGTGCAGGACCCTGAGAAAA TACCATCCACTGGCAAGTTGGTGCTGACACTCACAACCGACGCCTGTGAGGGGAAGGAAAATTTTGTCCG CTACCTGGAGCATGTCCAGGCTGTCATCACGGTCAACGCAACCAGAAGAGGAGACCTGAACATCAACATG ACTTCCCCTATGGGCACCAAGTCCATTTTGCTGAGCCGGCGTCCAAGGGATGACGACTCCAAGGTGGGCT TTGACAAGTGGCCTTTCATGACCACTCACACGTGGGGGGAAGACGCCCGAGGCACCTGGACCCTGGAGCT GGGATTTGTCGGCAGCGCCCCGCAGAAGGGGGTGCTGAAGGAGTGGACCCTGATGCTGCATGGCACTCAG AGTGCCCCGTACATCGACCAGGTGGTGCGGGATTACCAGTCCAAGTTGGCCATGTCCAAGAAAGAGGAGC TGGAGGAAGAGCTGGACGAAGCCGTGGAGAGAAGCCTGAAAAGCATCCTTAACAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233003 representing NM_001201529
Red=Cloning site Green=Tags(s) MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKVKMALQQEGFD RKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHP DLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPF MTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYD DCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSA AAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKM AKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINM TSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQ SAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001201529 |
| ORF Size | 1809 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001201529.3 |
| RefSeq Size | 4687 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 5126 |
| UniProt ID | P16519 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014] |
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