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FRMD3 (NM_001244959) Human Tagged ORF Clone
CAT＃: RG232965
- TrueORF®
FRMD3 (tGFP-tagged) - Homo sapiens FERM domain containing 3 (FRMD3), transcript variant 2
ORF Plasmid: DDK
"NM_001244959" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4.1O; EPB41L4O; EPB41LO; P410 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232965 representing NM_001244959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCCTCCTGCCACTGTGTGCCGAGAGGCAGGAGGACCATGAAAATGATCCACTTTCGGAGCTCCA GCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATCCGGCTGCTGGACGACTCGGAGATCTCCTGCCA CATCCAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCACATCTGCAACTACTACAGCCTGCTGGAGAAG GACTACTTTGGCATTCGCTATGTGGACCCAGAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCT TCAAGCAAATGAAAACTCATCCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTT GAAGATTAAAGAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCGC CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTTGGTGATTACG ATCCTGATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCCAAGCAGTCACAGAAGCTGGA AAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGGCAGAGCCCACCAGTTGCTGAATTTAACTTG CTCCTGAAAGCTCACACTTTGGAAACCTACGGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAA CAACATTTTTAGGATTCACAGCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAA ATGGCCAGATGTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGTGGAGTGGAAA ACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCAAGCAGCAAGATATTTTTTAA AGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAGGTGGTGGAGGCCAGTTCCAAGATCCAGAGG GAGCCTCCTGAGGTGCACAGAGCCAACATTACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCA TCATTAACATGGAACCCCTGCAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGG TGAGGGTGTTCCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTAACCATCTCTG AACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGACGATGAGATTGACATGCTCTT TGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACAGATTCATTTGAGGATCTGGAAGCAGATGAA AACGCCTTTTTGATTGCTGAAGAAGAGGAGCTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACA TTCTGACTGGCCATATTCGGGTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGG ACTGCTGCTCTTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232965 representing NM_001244959
Red=Cloning site Green=Tags(s) MFASCHCVPRGRRTMKMIHFRSSSVKSLSQEMRCTIRLLDDSEISCHIQRETKGQFLIDHICNYYSLLEK DYFGIRYVDPEKQRHWLEPNKSIFKQMKTHPPYTMCFRVKFYPHEPLKIKEELTRYLLYLQIKRDIFHGR LLCSFSDAAYLGACIVQAELGDYDPDEHPENYISEFEIFPKQSQKLERKIVEIHKNELRGQSPPVAEFNL LLKAHTLETYGVDPHPCKDSTGTTTFLGFTAAGFVVFQGNKRIHLIKWPDVCKLKFEGKTFYVIGTQKEK KAMLAFHTSTPAACKHLWKCGVENQAFYKYAKSSQIKTVSSSKIFFKGSRFRYSGKVAKEVVEASSKIQR EPPEVHRANITQSRSSHSLNKQLIINMEPLQPLLPSPSEQEEELPLGEGVPLPKEENISAPLISSSPVKA AREYEDPPSEEEDKIKEEPLTISELVYNPSASLLPTPVDDDEIDMLFDCPSRLELEREDTDSFEDLEADE NAFLIAEEEELKEARRALSWSYDILTGHIRVNPLVKSFSRLLVVGLGLLLFVFPLLLLLLESVSMQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001244959 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001244959.2 |
| RefSeq Size | 2103 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 257019 |
| UniProt ID | A2A2Y4 |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene is a single pass membrane protein primarily found in ovaries. A similar protein in erythrocytes helps determine the shape of red blood cells, but the function of the encoded protein has not been determined. There is some evidence that this is a tumor suppressor gene, and there is also evidence linking defects in this gene to susceptibility to diabetic nephropathy in type 1 diabetes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
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