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DCP2 (NM_001242377) Human Tagged ORF Clone
CAT＃: RG232630
- TrueORF®
DCP2 (tGFP-tagged) - Homo sapiens decapping mRNA 2 (DCP2), transcript variant 2
ORF Plasmid: DDK
"NM_001242377" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NUDT20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232630 representing NM_001242377
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAAACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATA TTCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTGGTTTTACTT GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGACTTTGCTAAAGCTGTCTTC AGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGAAAAAGTTTTGGATGAATGGAAGGAATATA AAATGGGAGTACCAACATATGGTGCAATTATTCTTGATGAGACACTTGAAAATGTACTACTAGTTCAGGG GTACCTAGCAAAATCAGGCTGGGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGT GCTGCTAGAGAGGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTG AACTTCGAATCAATGACCAGCTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACACAAAATTTAA CCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGAAATTGCCTTGTCATAGAAAT GATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACAAATTTTTTATGGCCATTCCCTTTATCAGAC CATTAAGGGACTGGCTTTCTCGAAGATTTGGCGATTCCTCAGACAGTGACAATGGATTTTCCTCAACTGG TAGCACGCCGGCTAAACCCACTGTGGAAAAATTGAGTCGAACCAAATTCCGCCACAGTCAGCAGTTATTT CCTGACGGTTCTCCTGGTGACCAGTGGGTAAAGCACAGGCAACCACTGCAGCAAAAGCCATATAATAATC ATTCTGAAATGTCTGACCTTTTAAAAGGAAAGAAGTGTGAAAAGAAACTTCATCCACGGAAACTTCAGGA TAATTTTGAAACAGATGCTGTATATGACTTGCCTAGCTCCAGTGAAGACCAGTTGCTAGAACATGCTGAG GGACAGCCCGTGGCATGTAATGGACATTGCAAGTTCCCCTTTTCATCCAGAGCCTTTTTGAGTTTCAAGT TTGACCATAATGCTATAATGAAAATCTTGGACCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232630 representing NM_001242377
Red=Cloning site Green=Tags(s) METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVF SHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDC AAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRN DMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHSQQLF PDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAE GQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001242377 |
| ORF Size | 1155 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001242377.2 |
| RefSeq Size | 10093 bp |
| RefSeq ORF | 1158 bp |
| Locus ID | 167227 |
| UniProt ID | Q8IU60 |
| Protein Pathways | RNA degradation |
| Gene Summary | The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011] |
Documents
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