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SPIB (NM_001243999) Human Tagged ORF Clone
CAT＃: RG231980
- TrueORF®
SPIB (tGFP-tagged) - Homo sapiens Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), transcript variant 3
ORF Plasmid: DDK
"NM_001243999" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SPI-B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231980 representing NM_001243999
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCCTGGAGGCTGCACAGCTCGACGGGCCACACTTCAGCTGTCTGTACCCAGATGGCGTCTTCT ATGACCTGGACAGCTGCAAGCATTCCAGCTACCCTGATTCAGAGGGGGCTCCTGACTCCCTGTGGGACTG GACTGTGGCCCCACCTGTCCCAGCCACCCCCTATGAAGCCTTCGACCCGGCAGCAGCCGCTTTTAGCCAC CCCCAGGCTGCCCAGCTCTGCTACGAACCCCCCACCTACAGCCCTGCAGGGAACCTCGAACTGGCCCCCA GCCTGGAGGCCCCGGGGCCTGGCCTCCCCGCATACCCCACGGAGAACTTCGCTAGCCAGACCCTGGTTCC CCCGGCATATGCCCCGTACCCCAGCCCTGTGCTATCAGAGGAGGAAGACTTACCGTTGGACAGCCCTGCC CTGGAGGTCTCGGACAGCGAGTCGGATGAGGCCCTCGTGGCTGGCCCCGAGGGGAAGGGATCCGAGGGAC TCGCAAGAAGCTGCGCCTGTACCAGTTCCTGCTGGGGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231980 representing NM_001243999
Red=Cloning site Green=Tags(s) MLALEAAQLDGPHFSCLYPDGVFYDLDSCKHSSYPDSEGAPDSLWDWTVAPPVPATPYEAFDPAAAAFSH PQAAQLCYEPPTYSPAGNLELAPSLEAPGPGLPAYPTENFASQTLVPPAYAPYPSPVLSEEEDLPLDSPA LEVSDSESDEALVAGPEGKGSEGLARSCACTSSCWGY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243999 |
| ORF Size | 531 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243999.1, NP_001230928.1 |
| RefSeq Size | 3533 bp |
| RefSeq ORF | 534 bp |
| Locus ID | 6689 |
| UniProt ID | Q01892 |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
