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eIF4GII (EIF4G3) (NM_001198801) Human Tagged ORF Clone
CAT＃: RG231488
- TrueORF®
EIF4G3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 1
ORF Plasmid: DDK
"NM_001198801" in other vectors (3)
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CNY 17,770.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | eIF-4G 3; eIF4G 3; eIF4GII |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231488 representing NM_001198801
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCTAGAGCTACCA TCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCAGTGTACCAGGCTAATCAGCA CATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGGCCTCAGTACTGTATACCACAG TACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTC CTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCC GGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAA ACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCTGGAGGTGGCA GCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCCTCCTCAGCTGCCCAGCCAGGT CCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACT GCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCC TTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGTATC CATAGCAGAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTTGCTCGAAGTACAATTGCAGCC CCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGATGTGAACTCTCAT CCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACAGAAACATCAGACCCTTTACCAAC AAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGTGTAGCACCTAATGATATTCCACTGGTTTCT AGTACTAACCTAATTAATGAAATAAATGGAGTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAA TAGTAAAACAGGAAGTATTGCCATTGACTCTTGAATTGGAGATTCTCGAAAATCCCCCAGAAGAAATGAA ACTGGAGTGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACTCCT CCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTTAGTTCTCCGAGTGCTG CCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTGCCTTAGTGAAGATGCAAAAGA GATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACAGAAGAGATTTTGGATTCTCAAAACTTAAAT TCAAGAAGGAGCCCTGTCCCAGCTCAAATAGCTATAACTGTACCAAAGACATGGAAGAAACCAAAAGATC GGACCCGAACCACTGAAGAGATGTTAGAGGCAGAATTGGAGCTTAAAGCTGAAGAGGAGCTTTCCATTGA CAAAGTACTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAAAGAGACCCCTCTGACCTA AAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGTGCTGAGAGTGTTT CTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCTGGTGATGGGGTTACATTTCCATT TAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGTAAGAAGCAGTATGACAGGGAGTTTCTGCTGGAC TTCCAGTTCATGCCTGCCTGTATACAAAAACCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTGACA AGATCAACCAACCCAAATTGCCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCAGACTTTAC ACCAGCCTTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTATTTGCAAAGTGCAGAGC AGGCATGGATTGCCAATTCTGGAACAGAGCAAAGCCCCAACTTGCCCTCCACTGGTGATGTCACACCCAC CCATGAAGAGCCTGCCTCTAGGGTTGTTGAATGTTGGGTCACGAAGATCTCAACCTGGCCAAAGAAGAGA ACCCAGAAAGATCATCACAGTTTCTGTAAAAGAAGATGTACACCTGAAAAAGGCAGAAAATGCCTGGAAG CCAAGCCAAAAACGAGACAGCCAAGCCGATGATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAG TTCGAAGTATCTTAAATAAATTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTTAC TGTTGACACAGAGGAGCGGCTGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATTGATGAACCCAGT TTCTCTGTGGCTTACGCAAACATGTGTCGATGTCTAGTAACGCTGAAAGTACCCATGGCAGACAAGCCTG GTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGTTGCCAGAAGGAGTTTGAAAAAGATAAAGCAGA TGATGATGTCTTTGAGAAGAAGCAGAAAGAACTTGAGGCTGCCAGTGCTCCAGAGGAGAGGACAAGGCTT CATGATGAACTGGAAGAAGCCAAGGACAAAGCCCGGCGGAGATCCATTGGCAACATCAAGTTTATTGGAG AACTCTTTAAACTCAAAATGCTGACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAACCA TGATGAAGAATCCCTGGAGTGCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTGGACTTTGAAAAA GCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGAGAAAATTGTGAAAGAAAGAAAAACCTCATCTA GGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGGCTGTGCAATTGGGTATCTCGAAGAGCAGATCA AGGGCCTAAAACTATCGAACAGATTCACAAAGAGGCTAAAATAGAAGAACAAGAAGAGCAAAGGAAGGTC CAGCAACTCATGACCAAAGAGAAGAGAAGACCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTG TACAAGGGGCCAAGAACAGTCGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATTGA TGAAAAAATTCAGCTGGTACCTAAAGCACAGCTAGGCAGCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAG GCAAGTGAGACTGATGCCTTACGGTCAAGTGCTTCCAGTTTAAACAGATTCTCTGCCCTGCAACCTCCAG CACCCTCAGGGTCCACGCCATCCACGCCTGTAGAGTTTGATTCCCGAAGGACCTTAACTAGTCGTGGAAG TATGGGCAGGGAGAAGAATGACAAGCCCCTTCCATCTGCAACAGCTCGGCCAAATACTTTCATGAGGGGT GGCAGCAGTAAAGACCTGCTAGACAATCAGTCTCAAGAAGAGCAGCGGAGAGAGATGCTGGAGACCGTGA AGCAGCTCACAGGAGGTGTGGATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAGTC AGCAAAACCAGAAATTTCAGCAATGTCAGCTCATGACAAGGCTGCATTATCAGAAGAGGAACTGGAGAGG AAGTCGAAATCTATCATTGATGAATTTCTACACATTAATGATTTTAAGGAAGCCATGCAGTGTGTGGAAG AGCTGAATGCCCAGGGCCTACTACATGTTTTTGTGAGAGTGGGAGTGGAGTCCACCCTGGAAAGGAGCCA GATCACCAGGGATCACATGGGCCAATTACTCTATCAGCTGGTACAGTCAGAAAAACTCAGCAAACAGGAC TTTTTCAAAGGTTTTTCAGAAACTTTGGAATTGGCAGATGACATGGCCATTGATATTCCCCATATTTGGT TGTACCTTGCTGAACTGGTGACCCCCATGTTAAAAGAAGGTGGAATCTCCATGAGAGAACTTACCATAGA ATTTAGCAAACCTTTACTTCCTGTTGGAAGAGCTGGGGTCTTGCTATCTGAAATATTGCACCTACTATGC AAACAAATGAGCCATAAGAAAGTGGGAGCCTTATGGAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTAC CAGAAGGAGAAGATGTACATAATTTTCTTTTGGAGCAGAAGTTGGACTTCATAGAGTCTGACAGTCCCTG TTCCTCTGAAGCACTTTCAAAGAAAGAACTGTCTGCCGAAGAGCTGTATAAGCGACTCGAGAAACTCATT ATTGAGGACAAAGCGAATGATGAACAGATCTTTGACTGGGTAGAGGCTAATCTAGACGAAATCCAGATGA GTTCACCTACATTCCTTAGAGCTTTAATGACTGCTGTTTGTAAAGCAGCTATTATAGCCGACTCTTCTAC CTTCAGAGTGGACACTGCTGTTATCAAGCAGAGAGTGCCGATCTTACTCAAGTACCTAGACTCAGATACA GAGAAGGAACTGCAAGCACTTTATGCACTACAAGCATCGATAGTAAAACTTGATCAACCTGCCAATTTGC TGCGGATGTTTTTTGATTGTCTATATGACGAGGAGGTGATCTCCGAGGATGCCTTCTACAAATGGGAGAG CAGCAAGGACCCTGCAGAGCAGAATGGGAAGGGCGTGGCTCTGAAATCTGTCACGGCATTCTTCACGTGG CTGCGGGAAGCAGAAGAGGAGTCTGAGGATAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231488 representing NM_001198801
Red=Cloning site Green=Tags(s) MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQ YRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKK TIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQLPSQVPEHSPVVYGTVESAHLAASTPVT AASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAA PTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLVS STNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTP PASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLN SRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVLESEQDKMSQGFHPERDPSDL KKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSGDGVTFPFKPESWKPTDTEGKKQYDREFLLD FQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPICKVQS RHGLPILEQSKAPTCPPLVMSHPPMKSLPLGLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWK PSQKRDSQADDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPS FSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRL HDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEK AKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKV QQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAK ASETDALRSSASSLNRFSALQPPAPSGSTPSTPVEFDSRRTLTSRGSMGREKNDKPLPSATARPNTFMRG GSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRESAKPEISAMSAHDKAALSEEELER KSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVRVGVESTLERSQITRDHMGQLLYQLVQSEKLSKQD FFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLC KQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDFIESDSPCSSEALSKKELSAEELYKRLEKLI IEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAAIIADSSTFRVDTAVIKQRVPILLKYLDSDT EKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTW LREAEEESEDN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198801 |
| ORF Size | 4863 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198801.2 |
| RefSeq Size | 6515 bp |
| RefSeq ORF | 4866 bp |
| Locus ID | 8672 |
| UniProt ID | O43432 |
| Protein Families | Transcription Factors |
| Protein Pathways | Viral myocarditis |
| Gene Summary | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231488 | EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 1 |
CNY 16,150.00 |
|
| RC231488L3 | Lenti-ORF clone of EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 1 |
CNY 18,050.00 |
|
| RC231488L4 | Lenti-ORF clone of EIF4G3 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 1 |
CNY 18,050.00 |
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