ODF2 (NM_153435) Human Tagged ORF Clone
CAT#: RG231435
- TrueORF®
ODF2 (tGFP-tagged) - Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1
ORF Plasmid: DDK
"NM_153435" in other vectors (3)
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CNY 7,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CT134; ODF2/1; ODF2/2; ODF84 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231435 representing NM_153435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGCTGCCGACCGGGTGTCGGAAAAGGAGGCGGAAGCGGGGAGGAGCCGCTGCCAGAGCCAGAC TGCATCCGCCGCGGCGTCTCCATGGCACGACCCTGGCCTCCGACTTCAACGACTTCATAAGGCGGCGTTT CTGGGCGCAGCCGTGTCGCTCCTGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCACGCAGAAA AAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAGCCAACCATGAAGGACCGCTCTT CAACTCCCCCCTTACATGTTCACGTGGATGAGAACACCCCTGTCCACGTCCACATAAAAAAACTCCCGAA ACCATCAGCGACCAGCAGCCAGAAATCTCACAAGCGAGGAATGAAAGGGGACACTGTGAATGTGCGGCGG AGTGTCCGGGTGAAAACCAAGAATCCACCTCATTGCCTGGAGATCACGCCACCATCTTCAGAAAAGCTGG TCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATGATGACTCAGGTCACTGTAAAATGAACCGTTA TGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTGTCTGAAGTCTGAGGTCAAGATGCAAAAAGGT GAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGGAGGTGGCCCACGAACTGG CTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAAGGAGGAGAAGGACTTCAC CATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAGCTGGTGGAGGCGGAAATG GATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACGGAGAAAC AAATGACCTGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGCACATT TGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAAGAGGATTCTGAAAGACTA ATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGAAAGCGCGCCTGCTGTTAC TGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATGTGAGAAGGCTCAAGCAAA GACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAGGCACAGCTTCGGTCCAAA GAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCGGGAATCAGCATAAGGCAG AAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCGAGACAAAGAGAGCTTGAA GAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCTGAGCAGCTACACGTGCAA CTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCTGGAGGAGCCGCTACAACC AAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGACCGGGTAACAGATCTTGT AAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTGGTGGAGAGACTACACCGT CAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCAGCTTTGCTCCAATGGAGG ACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAAGAACTATGAGGGGATGAT TGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTAGCTGCCCAGCTAGAACGC TGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGGCGGCACGAAGGCAGTTCC AGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGAGGCGAAGCTGGCTGAGTG CCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATCATATCAGACCTGCGCAGC CGGATCGAACACCAGGGGGACAAGCTGGAGATGGCGAGAGAGAAACATCAGGCTTCCCAGAAGGAAAATA AACAGCTGAGTCTGAAGGTGGATGAACTGGAGAGGAAACTGGAGGCGACCAGTGCCCAGAATATCGAGTT CCTACAGGTGATTGCCAAGAGGGAGGAGGCAATCCACCAGTCCCAGCTGCGGCTGGAGGAGAAAACACGG GAATGTGGGACCCTGGCAAGGCAGTTGGAGAGTGCCATTGAAGATGCGAGGAGGCAGGTGGAACAAACCA AGGAGCACGCACTCTCCAAGGAGCGAGCAGCCCAGAACAAAATCCTGGACCTTGAGACCCAGCTGAGCAG AACCAAAACGGAATTGAGCCAGCTGCGGCGGAGCCGTGATGATGCGGACCGCCGCTACCAGAGCCGGCTG CAAGACCTGAAAGATCGCCTGGAGCAGTCCGAGAGCACCAACCGCAGCATGCAGAACTACGTCCAGTTCC TCAAATCATCATACGCCAACGTGTTTGGGGATGGTCCCTATTCCACCTTCCTGACTAGCTCTCCCATCCG CTCCCGATCTCCTCCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231435 representing NM_153435
Red=Cloning site Green=Tags(s) MGELPTGCRKRRRKRGGAAARARLHPPRRLHGTTLASDFNDFIRRRFWAQPCRSWFPSCGKNGVTSLTQK KVLRAPCGAPSVTVTKPTMKDRSSTPPLHVHVDENTPVHVHIKKLPKPSATSSQKSHKRGMKGDTVNVRR SVRVKTKNPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQKG ERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSKLVEAEM DGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSERL MEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQAQLRSK EAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYAEQLHVQ LADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSLVERLHR QTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLER CDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRS RIEHQGDKLEMAREKHQASQKENKQLSLKVDELERKLEATSAQNIEFLQVIAKREEAIHQSQLRLEEKTR ECGTLARQLESAIEDARRQVEQTKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRL QDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153435 |
ORF Size | 2679 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153435.1, NP_702913.1 |
RefSeq Size | 3805 bp |
RefSeq ORF | 2682 bp |
Locus ID | 4957 |
UniProt ID | Q5BJF6 |
Gene Summary | The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231435 | ODF2 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 |
CNY 7,130.00 |
|
RC231435L3 | Lenti-ORF clone of ODF2 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 |
CNY 9,030.00 |
|
RC231435L4 | Lenti-ORF clone of ODF2 (mGFP-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 |
CNY 9,030.00 |