Aprataxin (APTX) (NM_001195250) Human Tagged ORF Clone
CAT#: RG231147
- TrueORF®
APTX (tGFP-tagged) - Human aprataxin (APTX), transcript variant 8
ORF Plasmid: DDK
"NM_001195250" in other vectors (3)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231147 representing NM_001195250
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAACGTGAATTTGTCCGTCTCCGACTTCTGGAGAGTGATGATGCGGGTGTGCTGGTTGGTGAGAC AGGACAGCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTGGCCCAGAGAC CAAGATCACTGATAAGAAATGTTCTCGACAGCAAGAGTTTGAGGAAGAGGCAAAGAACCCTGGCCTGGAA ACACACAGGAAGAGAAAGAGATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAAGCTGAGG CTGGGACAGGGCTGGAACCTGGGAGCAACTCTGGCCAATGCTCTGTGCCCCTAAAGAAGGGAAAAGATGC ACCTATCAAAAAGGAATCCCTGGGCCACTGGAGTCAAGGCTTGAAGATTTCTATGCAGGACCCCAAAATG CAGGTTTACAAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGCCCGTTACCATTGGCTGG TCTTACCGTGGACCTCCATTTCCAGTCTGAAGGCTGTGGCCAGGGAACACCTTGAACTCCTTAAGCATAT GCACACTGTGGGGGAAAAGGTGATTGTAGATTTTGCTGGGTCCAGCAAACTCCGCTTCCGATTGGGCTAC CACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTA AAAACAAAAAACATTGGAATTCTTTCAATACAGAATACTTCCTAGAATCACAAGCTGTGATCGAGATGGT ACAAGAGGCTGGTAGAGTAACTGTCCGAGATGGGATGCCTGAGCTCTTGAAGCTGCCCCTTCGTTGTCAT GAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAGCTGAAAGAACATCTCAGGAAGCACTGGACACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231147 representing NM_001195250
Red=Cloning site Green=Tags(s) MSNVNLSVSDFWRVMMRVCWLVRQDSRHQRIRLPHLEAVVIGRGPETKITDKKCSRQQEFEEEAKNPGLE THRKRKRSGNSDSIERDAAQEAEAGTGLEPGSNSGQCSVPLKKGKDAPIKKESLGHWSQGLKISMQDPKM QVYKDEQVVVIKDKYPKARYHWLVLPWTSISSLKAVAREHLELLKHMHTVGEKVIVDFAGSSKLRFRLGY HAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYFLESQAVIEMVQEAGRVTVRDGMPELLKLPLRCH ECQQLLPSIPQLKEHLRKHWTQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001195250 |
ORF Size | 906 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001195250.1, NP_001182179.1 |
RefSeq Size | 1848 bp |
RefSeq ORF | 867 bp |
Locus ID | 54840 |
UniProt ID | Q7Z2E3 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231147 | APTX (Myc-DDK-tagged)-Human aprataxin (APTX), transcript variant 8 |
CNY 3,600.00 |
|
RC231147L3 | Lenti ORF clone of Human aprataxin (APTX), transcript variant 8, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC231147L4 | Lenti ORF clone of Human aprataxin (APTX), transcript variant 8, mGFP tagged |
CNY 5,890.00 |
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