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Claspin (CLSPN) (NM_001190481) Human Tagged ORF Clone
CAT＃: RG230895
- TrueORF®
CLSPN (tGFP-tagged) - Human claspin (CLSPN), transcript variant 2
ORF Plasmid: DDK
"NM_001190481" in other vectors (2)
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CNY 13,970.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230895 representing NM_001190481
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGCGAGGTGGGTTCTGAGGTTCACCTAGAAATCAATGACCCAAACGTCATTTCACAAGAGGAAG CAGATAGTCCTTCAGATAGTGGACAGGGCGGCTATGAAACAATTGGACCCTTGAGTGAAGGAGATTCAGA TGAAGAGATATTTGTAAGTAAGAAGTTGAAAAACAGGAAGGTTCTACAAGACAGTGATTCCGAAACAGAG GACACAAATGCCTCTCCAGAGAAAACTACCTATGACAGTGCCGAGGAGGAAAATAAAGAGAATTTATATG CTGGGAAAAATACAAAAATCAAAAGGATTTACAAAACTGTGGCAGACAGTGATGAAAGTTACATGGAAAA GTCTTTGTATCAGGAAAATCTTGAAGCGCAAGTGAAACCTTGCTTAGAGCTGAGTCTTCAGTCTGGAAAC TCTACAGACTTTACCACTGACAGAAAGAGTTCCAAAAAGCACATACATGATAAAGAAGGAACTGCAGGAA AAGCAAAAGTAAAATCAAAAAGAAGACTTGAGAAAGAGGAGAGAAAAATGGAAAAAATTAGACAGCTAAA AAAGAAGGAAACAAAAAACCAGGAAGATGATGTAGAACAGCCATTTAATGACAGTGGCTGTCTTCTTGTG GATAAAGACCTTTTTGAAACTGGGTTGGAGGATGAAAATAACTCTCCATTGGAAGATGAAGAGTCATTAG AATCAATAAGAGCAGCTGTAAAAAACAAAGTAAAAAAGCACAAGAAAAAAGAACCATCTTTGGAGAGTGG GGTCCATTCATTTGAGGAAGGAAGTGAGTTATCAAAAGGAACCACGAGGAAGGAAAGAAAGGCAGCCAGA TTAAGTAAAGAAGCATTAAAACAACTGCATAGTGAGACTCAGCGCCTTATTCGAGAGTCTGCACTGAACC TTCCATATCATATGCCTGAGAATAAAACCATTCATGATTTCTTCAAACGTAAACCCCGGCCCACTTGCCA CGGAAATGCCATGGCACTATTGAAGTCATCTAAATATCAGTCAAGCCATCACAAAGAAATCATAGACACT GCAAATACTACTGAAATGAACAGTGATCACCATAGTAAAGGTTCTGAGCAGACAACAGGTGCAGAAAATG AAGTGGAAACTAATGCACTCCCTGTAGTTTCAAAGGAAACCCAGATCATTACTGGATCAGATGAGTCTTG CAGGAAGGATTTGGTAAAAAATGAAGAGCTAGAAATTCAGGAGAAACAGAAGCAGAGTGACATTAGACCT TCACCTGGGGACAGCTCAGTGTTGCAACAGGAATCCAACTTCCTCGGGAACAATCACAGTGAGGAATGTC AGGTTGAAGGGCTTGTAGCATTTGAACCTCATGCCCTGGAGGGTGAAGGCCCCCAAAATCCAGAAGAAAC AGATGAGAAAGTGGAAGAGCCTGAGCAGCAAAATAAATCATCAGCAGTTGGGCCACCTGAAAAAGTGAGA CGGTTTACTCTGGATAGACTTAAGCAACTGGGAGTAGATGTTTCCATTAAACCACGGCTAGGTGCTGATG AAGATTCCTTTGTGATACTTGAACCTGAAACCAACAGAGGTGAAAAGCTTCAGGTGTTAAAAGCTAAACT GCAAGAAGCAATGAAACTCCGAAGGTTTGAGGAGCGCCAGAAGCGCCAAGCACTGTTTAAATTAGATAAT GAAGATGGGTTTGAGGAAGAGGAGGAGGAAGAGGAAGAAATGACAGATGAGTCTGAGGAAGATGGAGAAG AGAAGGTAGAGAAAGAAGAGAAAGAGGAAGAACTAGAGGAAGAGGAGGAGAAAGAAGAGGAGGAGGAAGA AGAAGGAAATCAGGAGACTGCAGAATTCCTTCTTAGTAGTGAAGAAATAGAAACAAAAGATGAAAAAGAA ATGGATAAAGAAAATAATGATGGCAGTAGTGAAATTGGCAAGGCAGTTGGCTTCCTCTCTGTTCCCAAGT CTCTCTCATCAGATTCTACTTTACTTCTGTTTAAGGACAGCTCTTCCAAGATGGGTTACTTTCCTACTGA AGAAAAATCAGAAACAGATGAAAACTCAGGCAAGCAGCCTAGCAAACTGGATGAGGATGATTCATGTTCA TTGCTAACAAAGGAGAGCAGCCACAATAGCAGCTTTGAGCTGATTGGCTCCACGATTCCATCCTATCAGC CTTGCAACAGACAAACAGGCCGTGGGACCAGTTTTTTCCCTACAGCAGGAGGATTCAGATCTCCTTCCCC TGGGCTATTTCGAGCCAGTTTGGTCAGCTCAGCTTCTAAGAGTTCAGGGAAACTGTCTGAGCCTTCACTT CCCATAGAGGATTCCCAGGATCTGTATAACGCCTCCCCAGAGCCTAAGACACTTTTCCTAGGAGCAGGAG ACTTCCAGTTCTGTTTAGAAGATGACACTCAGAGCCAACTGTTGGATGCAGATGGGTTCTTAAATGTTAG AAACCACAGGAATCAGTACCAAGCTTTGAAGCCTCGATTGCCATTGGCCAGTATGGATGAGAATGCCATG GATGCCAACATGGATGAGCTGTTGGATTTGTGTACTGGAAAGTTCACATCTCAGGCTGAAAAACATCTAC CCAGGAAGGGTGACGAGAAAGAGAACATGGAGGAACTTCTGAACCTTTGTTCAGGAAAATTCACTTCTCA GGATGCCTCCACTCCAGCCTCATCAGAGTTAAATAAACAGGAGAAGGAGAGCAGCATGGGTGATCCAATG GAAGAAGCACTTGCTCTTTGCTCAGGCTCTTTTCCCACAGACAAGGAAGAGGAAGACGAGGAGGAGGAAT TTGGAGACTTTCGGCTTGTTTCAAATGATAATGAGTTTGATAGTGATGAGGATGAACACAGTGACTCTGG TAATGATCTGGCACTGGAAGACCATGAAGATGATGATGAAGAAGAACTCCTGAAGCGATCTGAGAAGTTG AAAAGGCAAATGAGGTTGAGGAAATACCTGGAGGATGAGGCAGAGGTGTCAGGAAGTGATGTGGGAAGCG AAGATGAGTATGATGGGGAAGAAATTGATGAATATGAAGAGGACGTAATTGATGAAGTACTTCCTTCTGA TGAGGAACTGCAGAGTCAAATCAAGAAAATACACATGAAAACTATGTTGGATGATGATAAGCGACAGCTA CGTTTATACCAAGAGAGGTACCTTGCTGATGGGGATCTACACAGCGATGGTCCTGGGCGAATGAGGAAGT TTCGATGGAAAAACATAGATGATGCTTCCCAGATGGACTTGTTCCACAGAGACTCTGATGATGATCAGAC TGAAGAACAGCTTGATGAGTCAGAAGCCAGGTGGAGGAAGGAGCGAATTGAACGAGAGCAGTGGCTTCGG GACATGGCACAGCAGGGGAAAATTACAGCTGAAGAAGAAGAAGAAATTGGGGAGGACAGTCAGTTTATGA TACTGGCCAAGAAAGTTACAGCCAAAGCACTGCAGAAGAATGCCAGTCGCCCTATGGTTATTCAGGAATC AAAGTCTTTGCTCAGAAATCCTTTTGAAGCCATCAGACCAGGAAGTGCTCAACAGGTGAAGACAGGCTCA CTGCTAAACCAGCCCAAAGCTGTGCTTCAGAAACTGGCTGCTCTCTCTGACCATAACCCCAGTGCTCCTC GAAATTCGAGAAACTTTGTCTTTCATACACTTTCTCCTGTCAAGGCTGAGGCGGCAAAGGAATCGTCTAA GTCTCAGGTAAAGAAAAGGGGTCCATCTTTCATGACTTCTCCTTCACCTAAGCACCTCAAAACAGATGAT AGCACTTCAGGATTGACGCGAAGCATCTTCAAATATTTGGAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230895 representing NM_001190481
Red=Cloning site Green=Tags(s) MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGGYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETE DTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGN STDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLV DKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAAR LSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDT ANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRP SPGDSSVLQQESNFLGNNHSEECQVEGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVR RFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDN EDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKE MDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCS LLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSL PIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAM DANMDELLDLCTGKFTSQAEKHLPRKGDEKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPM EEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKL KRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQL RLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLR DMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGS LLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDD STSGLTRSIFKYLES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190481 |
| ORF Size | 3825 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190481.1, NP_001177410.1 |
| RefSeq Size | 8292 bp |
| RefSeq ORF | 3828 bp |
| Locus ID | 63967 |
| UniProt ID | Q9HAW4 |
| Gene Summary | The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230895 | CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 2 |
CNY 8,880.00 |
|
| RC230895L3 | Lenti-ORF clone of CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 2 |
CNY 11,280.00 |
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