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AFF2 (NM_001169123) Human Tagged ORF Clone
CAT＃: RG230695
- TrueORF®
AFF2 (tGFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 3
ORF Plasmid: DDK
"NM_001169123" in other vectors (4)
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CNY 14,350.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230695 representing NM_001169123
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA AGCTGTGTTGAAGAAATCTTGCGGGAGATGACCCATTCCTGGCCTACTCCTCTCACTTCCATGCATACTG CTGGACACTCTGAGCAGAGCACCTTTTCCATCCCAGGACAGGAATCGCAGCATCTGACCCCAGGATTCAC CTTACAAAAGTGGAATGACCCAACCACCAGAGCTTCTACAAAGATGCTTGAGGATGACCTGAAGCTGAGC AGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAGG CTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTGC AGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACACT GAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCCT CAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTGG CCAAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGATG AAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGGG AGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAAC TAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCACT GACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTTC ATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTAA CATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATTC ATTGAAACAGATTCATCTACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTGC CTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCAG CACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACCT ATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTGA AGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAGA CCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAAG GGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAGG CCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAGA AAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACTG CCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATCG CCATGACTGGACAAATCACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAGG GATATCGGTAAATGAGGGAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCT ATTGCCACTGCTACTGTCACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGG CCACCACCACAACTACTACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAG CAGTCACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGA CCCAAGCTCACTTTTGATGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGC ACAAAGCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCAC TGAATGTGGCAATGCCATGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACT GTGGAGCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAA AGCTAGCAGTACTATGCTACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCA TGCTATGAAGTACTCCAGATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCC TCTCCATGGGTAAGCAATGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACG CAATGGGGAACTGTAACAATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGT CAACATCACTAGCAATGTGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAAC AAAGAATTCTTTGGTGATCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTG TCCGCTACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230695 representing NM_001169123
Red=Cloning site Green=Tags(s) MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP SCVEEILREMTHSWPTPLTSMHTAGHSEQSTFSIPGQESQHLTPGFTLQKWNDPTTRASTKMLEDDLKLS SDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDT ESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQM KVKTNASQVPAEPKERPLLSLIREKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTST DEFTWPKPNITSSTPKEKESVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREF IETDSSTSDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSP IPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPK GKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPL PEDPPRRRNVSGNNGPFGQDKNIAMTGQITSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNTA IATATVTATAIVTTTVTATATATATTTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRR PKLTFDDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSET VELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIP SPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTREN KEFFGDLDTLMGPLTQHSSMTNLVRYVRQGLCWLRIDAHLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001169123 |
| ORF Size | 3903 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001169123.2 |
| RefSeq Size | 13716 bp |
| RefSeq ORF | 3906 bp |
| Locus ID | 2334 |
| UniProt ID | P51816 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230695 | AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 3 |
CNY 13,020.00 |
|
| RC230695L3 | Lenti-ORF clone of AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 3 |
CNY 14,920.00 |
|
| RC230695L4 | Lenti-ORF clone of AFF2 (mGFP-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 3 |
CNY 14,920.00 |
|
| SC329333 | AFF2 (untagged)-Human AF4/FMR2 family member 2 (AFF2) transcript variant 3 |
CNY 10,450.00 |
