MIB2 (NM_001170689) Human Tagged ORF Clone
CAT#: RG230478
- TrueORF®
MIB2 (tGFP-tagged) - Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 5
ORF Plasmid: DDK
"NM_001170689" in other vectors (4)
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CNY 8,650.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ZZANK1; ZZZ5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230478 representing NM_001170689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCAGACCCCCAGGCGGGCGTGCAGGTGGGCATGCGGGTGGTGCGCGGCGTGGACTGGAAGTGGG GCCAGCAGGACGGCGGCGAGGGCGGCGTGGGCACGGTGGTGGAGCTTGGCCGCCACGGCAGCCCCTCGAC ACCCGACCGCACAGTGGTCGTGCAGTGGGACCAGGGCACGCGCACCAACTACCGCGCCGGCTACCAGGGC GCGCACGACCTGCTGCTGTACGACAACGCCCAGATCGGCGTCCGGCACCCCAACATCATCTGTGACTGCT GCAAGAAGCACGGGCTGCGGGGGATGCGCTGGAAGTGCCGTGTGTGCCTGGACTACGACCTCTGCACGCA GTGCTACATGCACAACAAGCATGAGCTCGCCCACGCCTTCGACCGCTACGAGACCGCTCACTCGCGCCCT GTCACACTGAGTCCCCGCCAGGGCCTCCCGAGGATCCCACTAAGGGGCATCTTCCAGGGAGCGAAGGTGG TGCGAGGCCCCGACTGGGAGTGGGGCTCACAGGATGGCAAGCCGGCGGAGCTGCAGCGCAGGGTGAGTGC TGACAGCCAGCCCTTCCAGCACGGGGACAAGGTCAAGTGTCTGCTGGACACTGATGTCCTGCGGGAGATG CAGGAAGGCCACGGCGGCTGGAACCCCAGGATGGCGGAGTTTATCGGACAGACGGGCACCGTGCATCGTA TCACGGACCGCGGGGACGTGCGCGTGCAGTTCAACCACGAGACGCGCTGGACCTTCCACCCCGGGGCGCT CACCAAGCACCACTCCTTCTGGGTGGGCGACGTGGTCCGGGTCATCGGCGACCTTGACACAGTGAAGCGG CTGCAGGCTGGGCATGGCGAGTGGACGGACGACATGGCCCCTGCCCTGGGCCGCGTCGGGAAGGTGGTGA AAGTGTTTGGAGACGGGAACCTGCGTGTAGCAGTCGCTGGTCAGCGGTGGACCTTCAGCCCCTCCTGCCT GGTGGCCTACCGGCCCGAGGAGGATGCCAACCTGGACGTGGCCGAGCGCGCCCGGGAGAACAAAAGCTCA CTGAGCGTGGCCCTGGACAAGCTTCGGGCCCAGAAGAGTGACCCAGAGCACCCGGGAAGGCTGGTGGTGG AGGTGGCGCTGGGTAACGCAGCCCGGGCTCTGGACCTGCTGCGGAGGCGCCCAGAGCAGGTGGACACCAA GAACCAAGGCAGGACCGCTCTGCAAGTGGCTGCCTACCTGGGCCAGGTGGAGTTGATACGGCTGCTGCTA CAAGCCAGGGCGGGCGTGGACCTGCCGGACGACGAGGGCAACACGGCACTGCACTACGCGGCCCTGGGGA ACCAGCCCGAGGCCACCAGGGTGCTCCTGAGTGCTGGGTGCCGGGCGGACGCCATCAACAGCACCCAGAG CACAGCACTGCACGTGGCCGTGCAGAGGGGCTTCCTGGAGGTGGTGCGGGCCCTGTGTGAGCGCGGCTGT GACGTCAACCTGCCCGACGCCCACTCGGACACGCCCCTGCACTCCGCCATCTCGGCGGGCACTGGAGCCA GCGGCATTGTCGAGGTCCTCACGGAGGTGCCAAACATCGATGTTACCGCCACCAACAGCCAGGGTTTCAC CCTGCTGCACCATGCCTCCCTCAAGGGTCACGCGCTAGCTGTGAGAAAGATTCTGGCTCGGGCGCGGCAG CTGGTGGACGCCAAGAAGGAGGACGGCTTCACGGCGCTGCATCTGGCTGCCCTCAACAACCACCGCGAGG TGGCCCAGATCCTCATCCGGGAGGGCCGCTGTGACGTGAACGTGCGCAACCGGAAGCTGCAGTCCCCGCT GCATCTCGCCGTGCAACAGGCCCACGTGGGGCTGGTGCCGCTACTGGTGGACGCTGGGTGCAGTGTCAAC GCCGAGGACGAGGAGGGGGACACAGCCCTGCACGTGGCGCTGCAGCGTCATCAGCTGCTGCCCCTGGTGG CTGATGGGGCCGGGGGGGACCCAGGGCCCTTGCAGCTGCTGTCCAGGCTACAGGCCTCGGGCCTCCCCGG CAGCGCGGAGCTGACGGTGGGCGCGGCGGTCGCCTGCTTCCTGGCGCTGGAGGGCGCCGACGTGAGCTAC ACCAACCACCGCGGTCGGAGCCCGCTGGACCTGGCCGCCGAGGGTCGCGTGCTCAAGGCCCTTCAGGGCT GCGCCCAGCGCTTCCGAGTGCGCGCGCAGGATGAAGAAGTGCATCAGGTGCCAGGTGGTCGTCAGCAAGA AACTGCGCCCAGACGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230478 representing NM_001170689
Red=Cloning site Green=Tags(s) MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQG AHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRP VTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREM QEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKR LQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSS LSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLL QARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGC DVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQ LVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVN AEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSY TNHRGRSPLDLAAEGRVLKALQGCAQRFRVRAQDEEVHQVPGGRQQETAPRRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001170689 |
ORF Size | 2259 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001170689.2 |
RefSeq Size | 2948 bp |
RefSeq ORF | 2262 bp |
Locus ID | 142678 |
UniProt ID | Q96AX9 |
Gene Summary | The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230478 | MIB2 (Myc-DDK-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 5 |
CNY 7,890.00 |
|
RC230478L3 | Lenti-ORF clone of MIB2 (Myc-DDK-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 5 |
CNY 9,790.00 |
|
RC230478L4 | Lenti-ORF clone of MIB2 (mGFP-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 5 |
CNY 9,790.00 |
|
SC329116 | MIB2 (untagged)-Human mindbomb homolog 2 (Drosophila) (MIB2) transcript variant 5 |
CNY 14,540.00 |