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SMOC2 (NM_001166412) Human Tagged ORF Clone
CAT＃: RG230096
- TrueORF®
SMOC2 (tGFP-tagged) - Human SPARC related modular calcium binding 2 (SMOC2), transcript variant 2
ORF Plasmid: DDK
"NM_001166412" in other vectors (2)
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CNY 4,940.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA37D8.1; bA270C4A.1; dJ421D16.1; DTDP1; MST117; MSTP117; MSTP140; SMAP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230096 representing NM_001166412
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCCCCCAGCTCTGCTGGCTGCCGCTGCTCGCTGGGCTGCTCCCGCCGGTGCCCGCTCAGAAGT TCTCGGCGCTCACGTTTTTGAGAGTGGATCAAGATAAAGACAAGGATTGTAGCTTGGACTGTGCGGGTTC GCCCCAGAAACCTCTCTGCGCATCTGACGGAAGGACCTTCCTTTCCCGTTGTGAATTTCAACGTGCCAAG TGCAAAGATCCCCAGCTAGAGATTGCATATCGAGGAAACTGCAAAGACGTGTCCAGGTGTGTGGCCGAAA GGAAGTATACCCAGGAGCAAGCCCGGAAGGAGTTTCAGCAAGTGTTCATTCCTGAGTGCAATGACGACGG CACCTACAGTCAGGTCCAGTGTCACAGCTACACGGGATACTGCTGGTGCGTCACGCCCAACGGGAGGCCC ATCAGCGGCACTGCCGTGGCCCACAAGACGCCCCGGTGCCCGGGTTCCGTAAATGAAAAGTTACCCCAAC GCGAAGGCACAGGAAAAACAGATGATGCCGCAGCTCCAGCGTTGGAGACTCAGCCTCAAGGAGATGAAGA AGATATTGCATCACGTTACCCTACCCTTTGGACTGAACAGGTTAAAAGTCGGCAGAACAAAACCAATAAG AATTCAGTGTCATCCTGTGACCAAGAGCACCAGTCTGCCCTGGAGGAAGCCAAGCAGCCCAAGAACGACA ATGTGGTGATCCCTGAGTGTGCGCACGGCGGCCTCTACAAGCCAGTGCAGTGCCACCCCTCCACGGGGTA CTGCTGGTGCGTCCTGGTGGACACGGGGCGCCCCATTCCCGGCACATCCACAAGGTACGAGCAGCCGAAA TGTGACAACACGGCCAGGGCCCACCCAGCCAAAGCCCGGGACCTGTACAAGGGCCGCCAGCTACAAGGTT GTCCGGGTGCCAAAAAGCATGAGTTTCTGACCAGCGTTCTGGACGCGCTGTCCACGGACATGGTCCACGC CGCCTCCGACCCCTCCTCCTCGTCAGGCAGGCTCTCAGAACCCGACCCCAGCCATACCCTAGAGGAGCGG GTGGTGCACTGGTACTTCAAACTACTGGATAAAAACTCCAGTGGAGACATCGGCAAAAAGGAAATCAAAC CCTTCAAGAGGTTCCTTCGCAAAAAATCAAAGCCCAAAAAATGTGTGAAGAAGTTTGTTGAATACTGTGA CGTGAATAATGACAAATCCATCTCCGTACAAGAACTGATGGGCTGCCTGGGCGTGGCGAAAGAGGACGGC AAAGCGGACACCAAGAAACGCCACACCCCCAGAGGTCATGCTGAAAGTACGTCTAATAGACAGCCAAGGA AACAAGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230096 representing NM_001166412
Red=Cloning site Green=Tags(s) MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGRTFLSRCEFQRAK CKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDDGTYSQVQCHSYTGYCWCVTPNGRP ISGTAVAHKTPRCPGSVNEKLPQREGTGKTDDAAAPALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNK NSVSSCDQEHQSALEEAKQPKNDNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPK CDNTARAHPAKARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLSEPDPSHTLEER VVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQELMGCLGVAKEDG KADTKKRHTPRGHAESTSNRQPRKQG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166412 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001166412.2 |
| RefSeq Size | 3117 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 64094 |
| UniProt ID | Q9H3U7 |
| Protein Families | Secreted Protein |
| Gene Summary | This gene encodes a member of the SPARC family (secreted protein acidic and rich in cysteine/osteonectin/BM-40), which are highly expressed during embryogenesis and wound healing. The gene product is a matricellular protein which promotes matrix assembly and can stimulate endothelial cell proliferation and migration, as well as angiogenic activity. Associated with pulmonary function, this secretory gene product contains a Kazal domain, two thymoglobulin type-1 domains, and two EF-hand calcium-binding domains. The encoded protein may serve as a target for controlling angiogenesis in tumor growth and myocardial ischemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Documents
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