TSSK4 (NM_001184739) Human Tagged ORF Clone

CAT#: RG229905

  • TrueORF®

TSSK4 (tGFP-tagged) - Human testis-specific serine kinase 4 (TSSK4), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001184739" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


TSSK4 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms C14orf20; STK22E; TSK-4; TSK4; TSSK5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG229905 representing NM_001184739
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGGGAGATGTCTTAGAGGCAGCACCAACCACCACAGCCTACCATTCCCTCATGGATGAATATG
GTTATGAGGTGGGCAAGGCCATTGGCCATGGCTCCTATGGGTCGGTATATGAGGCTTTCTACACAAAGCA
GAAGGTTATGGTGGCAGTCAAGATCATCTCAAAGAAGAAGGCCTCTGATGACTATCTTAACAAGTTCCTG
CCCCGTGAAATACAGGTAATGAAAGTCTTGCGGCACAAGTACCTCATCAACTTCTATCGGGCCATTGAGA
GCACATCTCGAGTATACATCATTCTGGAACTGGCTCAGGGTGGTGATGTCCTTGAATGGATCCAGCGCTA
CGGGGCCTGCTCTGAGCCCCTTGCTGGCAAGTGGTTCTCCCAGCTGACCCTGGGCATTGCCTACCTGCAC
AGCAAGAGCATCGTGCACCGCCTGATGCCCAGCCTTTCTGCTGCTGGTAGGGACTTAAAGTTGGAGAACC
TGTTGCTGGACAAGTGGGAGAATGTGAAGATATCAGACTTTGGCTTTGCCAAGATGGTGCCTTCTAACCA
GCCTGTGGGTTGTAGCCCTTCTTACCGCCAAGTGAACTGCTTTTCCCACCTCAGCCAGACTTACTGTGGC
AGCTTTGCTTACGCTTGCCCAGAGATCTTACGAGGCTTGCCCTACAACCCTTTCCTGTCTGACACCTGGA
GCATGGGCGTCATCCTTTACACTCTAGTGGTCGCCCATCTGCCCTTTGATGACACCAATCTCAAAAAGCT
GCTAAGAGAGACTCAGAAGGAGGTCACTTTCCCAGCTAACCATACCATCTCCCAGGAGTGCAAGAACCTG
ATCCTCCAGATGCTACGCCAAGCCACTAAGCGTGCCACCATTCTGGACATCATCAAGGATTCCTGGGTGC
TCAAGTTCCAGCCTGAGCAACCCACCCATGAGATCAGGCTGCTTGAGGCCATGTGCCAGCTCCACAACAC
CACTAAACAGCACCAATCCTTGCAAATTACGACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG229905 representing NM_001184739
Red=Cloning site Green=Tags(s)

MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFYTKQKVMVAVKIISKKKASDDYLNKFL
PREIQVMKVLRHKYLINFYRAIESTSRVYIILELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLH
SKSIVHRLMPSLSAAGRDLKLENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCG
SFAYACPEILRGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQECKNL
ILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001184739
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001184739.2
RefSeq Size 1330 bp
RefSeq ORF 1017 bp
Locus ID 283629
UniProt ID Q6SA08
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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