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ZNF135 (NM_003436) Human Tagged ORF Clone
CAT＃: RG229331
- TrueORF®
ZNF135 (tGFP-tagged) - Human zinc finger protein 135 (ZNF135), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_003436" in other vectors (3)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | pHZ-17; pT3; ZNF61; ZNF78L1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229331 ORF sequence, codon optimized.
Due to the complexity of NM_003436, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACCCGGGGTGCGCGTGAGCACAGATCCCGAGCAGGTAACTTTTGAGGACGTGGTGGTCGGGTTCA GCCAGGAAGAATGGGGACAGTTGAAGCCTGCTCAGCGAACACTGTACCGGGATGTGATGCTCGACACATT CCGACTGTTGGTCAGCGTGGGCCACTGGCTTCCTAAACCCAACGTGATCAGCCTGCTGGAGCAGGAGGCA GAACTTTGGGCCGTGGAATCACGCCTCCCCCAGGGAGTGTATCCCGAGATCAAGGGCCATTTCCAGTTTT TGCTCCTGTCAGACCTTGAGACACGACCTAAGGTTAAGCTGAGCGTTCTTAAGCAGGGAATCAGCGAGGA AATAAGTAATAGCGTTATCCTCGTCGAGCGCTTCTTGTGGGATGGCCTGTGGTACTGCCGGGGGGAGGAT ACCGAAGGCCATTGGGAGTGGAGTTGTGAATCACTCGAGAGCCTCGCCGTGCCAGTTGCTTTCACTCCCG TAAAAACCCCTGTGCTCGAACAGTGGCAAAGAAATGGCTTCGGAGAGAACATCTCACTGAATCCAGACCT CCCCCACCAACCTATGACCCCTGAGAGGCAATCTCCCCACACATGGGGAACCCGGGGCAAGAGGGAGAAG CCTGACTTGAATGTCCTTCAGAAAACTTGCGTGAAGGAGAAACCCTATAAATGTCAAGAGTGTGGCAAAG CATTCTCACACAGCAGCGCACTGATAGAACACCACCGGACTCATACTGGCGAGAGGCCGTACGAGTGCCA TGAATGCCTCAAAGGATTTCGCAATTCTAGCGCTCTGACAAAGCACCAGCGAATTCATACTGGCGAGAAG CCCTATAAGTGCACTCAGTGTGGAAGGACATTCAATCAGATAGCCCCCCTGATTCAACACCAGCGCACAC ACACAGGCGAAAAACCGTACGAGTGTAGCGAATGCGGCAAAAGTTTCTCCTTTAGAAGCTCTTTCTCTCA ACACGAAAGAACTCATACCGGGGAGAAGCCATATGAGTGCTCCGAATGCGGCAAAGCCTTCAGGCAGTCT ATTCATCTGACTCAGCACTTGCGAATACATACAGGCGAGAAGCCTTACCAGTGTGGGGAGTGTGGCAAGG CCTTTAGTCACAGTAGCAGTCTTACCAAACACCAAAGGATTCATACTGGAGAGAAACCATATGAGTGTCA CGAGTGTGGCAAAGCCTTCACTCAGATCACCCCACTCATACAGCACCAGAGAACACATACTGGCGAGAAA CCATATGAGTGCGGTGAATGCGGCAAAGCATTCTCCCAGTCCACACTTCTGACTGAACACAGAAGGATCC ACACCGGGGAAAAACCCTATGGGTGTAATGAATGCGGCAAGACTTTCAGCCACTCTAGCTCTCTGTCTCA ACATGAACGAACCCACACCGGCGAAAAGCCATATGAGTGCTCCCAATGCGGAAAGGCATTCCGACAGTCA ACGCATCTTACCCAGCACCAGAGAATCCACACGGGTGAGAAACCCTATGAGTGCAACGACTGCGGCAAGG CTTTTTCTCATTCCAGTTCATTGACAAAGCATCAAAGGATCCACACCGGAGAGAAACCTTATGAGTGTAA CCAGTGCGGGAGAGCCTTCAGTCAGCTTGCTCCCTTGATTCAACACCAAAGAATCCACACCGGGGAGAAA CCATACGAGTGCAACCAATGCGGGAGGGCTTTCTCTCAAAGCTCCCTCCTTATTGAGCATCAGAGAATAC ATACAAAGGAGAAGCCATACGGATGCAATGAGTGCGGCAAGTCTTTTAGCCACAGTAGCAGTCTGTCACA ACACGAGCGCACCCACACAGGTGAAAAGCCTTATGAGTGTCATGACTGCGGTAAGTCCTTTAGACAGTCT ACCCATCTTACCCAGCACAGGCGGATCCACACCGGCGAAAAGCCGTACGCATGCCGGGATTGCGGAAAAG CTTTCACCCACTCATCATCCCTGACAAAACATCAGCGAACCCATACCGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229331 representing NM_003436
Red=Cloning site Green=Tags(s) MTPGVRVSTDPEQVTFEDVVVGFSQEEWGQLKPAQRTLYRDVMLDTFRLLVSVGHWLPKPNVISLLEQEA ELWAVESRLPQGVYPEIKGHFQFLLLSDLETRPKVKLSVLKQGISEEISNSVILVERFLWDGLWYCRGED TEGHWEWSCESLESLAVPVAFTPVKTPVLEQWQRNGFGENISLNPDLPHQPMTPERQSPHTWGTRGKREK PDLNVLQKTCVKEKPYKCQECGKAFSHSSALIEHHRTHTGERPYECHECLKGFRNSSALTKHQRIHTGEK PYKCTQCGRTFNQIAPLIQHQRTHTGEKPYECSECGKSFSFRSSFSQHERTHTGEKPYECSECGKAFRQS IHLTQHLRIHTGEKPYQCGECGKAFSHSSSLTKHQRIHTGEKPYECHECGKAFTQITPLIQHQRTHTGEK PYECGECGKAFSQSTLLTEHRRIHTGEKPYGCNECGKTFSHSSSLSQHERTHTGEKPYECSQCGKAFRQS THLTQHQRIHTGEKPYECNDCGKAFSHSSSLTKHQRIHTGEKPYECNQCGRAFSQLAPLIQHQRIHTGEK PYECNQCGRAFSQSSLLIEHQRIHTKEKPYGCNECGKSFSHSSSLSQHERTHTGEKPYECHDCGKSFRQS THLTQHRRIHTGEKPYACRDCGKAFTHSSSLTKHQRTHTG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003436 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003436.3, NP_003427.3 |
| RefSeq Size | 3408 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 7694 |
| UniProt ID | P52742 |
| Domains | zf-C2H2 |
| Protein Families | Transcription Factors |
| Gene Summary | Plays a role in the regulation of cell morphology and cytoskeletal organization. May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229331 | ZNF135 (Myc-DDK-tagged)-Human zinc finger protein 135 (ZNF135), transcript variant 2. Note: ORF is codon optimized |
CNY 4,912.00 |
|
| SC117973 | ZNF135 (untagged)-Human zinc finger protein 135 (ZNF135), transcript variant 2 |
CNY 7,912.00 |
|
| SC327966 | ZNF135 (untagged)-Human zinc finger protein 135 (ZNF135) transcript variant 2 |
CNY 13,020.00 |
