PTGR1 (NM_001146108) Human Tagged ORF Clone
CAT#: RG228825
- TrueORF®
PTGR1 (tGFP-tagged) - Human prostaglandin reductase 1 (PTGR1), transcript variant 1
ORF Plasmid: DDK
"NM_001146108" in other vectors (4)
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CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DIG-1; LTB4DH; PGR1; ZADH3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228825 representing NM_001146108
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCGTACTAAGACATGGACCCTGAAGAAGCACTTTGTTGGCTATCCTACTAATAGTGACTTTGAGT TGAAGACATCTGAGCTCCCACCCTTAAAAAATGGAGAGGTCCTGCTTGAAGCTTTGTTCCTCACCGTGGA TCCCTACATGAGAGTGGCAGCCAAAAGATTGAAGGAAGGTGATACAATGATGGGGCAGCAAGTGGCCAAA GTTGTGGAAAGTAAAAATGTAGCCCTACCAAAAGGAACTATTGTACTGGCTTCTCCAGGCTGGACAACGC ACTCCATTTCTGATGGGAAAGATCTGGAAAAGCTGCTGACAGAGTGGCCAGACACAATACCACTGTCTTT GGCTCTGGGGACAGTTGGCATGCCAGGCCTGACTGCCTACTTTGGCCTACTTGAAATCTGTGGTGTGAAG GGTGGAGAAACAGTGATGGTTAATGCAGCAGCTGGAGCTGTGGGCTCAGTCGTGGGGCAGATTGCAAAGC TCAAGGGCTGCAAAGTTGTTGGAGCAGTAGGGTCTGATGAAAAGGTTGCCTACCTTCAAAAGCTTGGATT TGATGTCGTCTTTAACTACAAGACGGTAGAGTCTTTGGAAGAAACCTTGAAGAAAGCGTCTCCTGATGGT TATGATTGTTATTTTGATAATGTAGGTGGAGAGTTTTCAAACACTGTTATCGGCCAGATGAAGAAATTTG GAAGGATTGCCATATGTGGAGCCATCTCTACATATAACAGAACCGGCCCACTTCCCCCAGGCCCACCCCC AGAGATTGTTATCTATCAGGAGCTTCGCATGGAAGCTTTTGTCGTCTACCGCTGGCAAGGAGATGCCCGC CAAAAAGCTCTGAAGGACTTGCTGAAATGGGTCTTAGAGGGTAAAATCCAGTACAAGGAATATATCATTG AAGGATTTGAAAACATGCCAGCCGCATTTATGGGAATGCTGAAAGGAGATAATTTGGGGAAGACAATAGT GAAAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228825 representing NM_001146108
Red=Cloning site Green=Tags(s) MVRTKTWTLKKHFVGYPTNSDFELKTSELPPLKNGEVLLEALFLTVDPYMRVAAKRLKEGDTMMGQQVAK VVESKNVALPKGTIVLASPGWTTHSISDGKDLEKLLTEWPDTIPLSLALGTVGMPGLTAYFGLLEICGVK GGETVMVNAAAGAVGSVVGQIAKLKGCKVVGAVGSDEKVAYLQKLGFDVVFNYKTVESLEETLKKASPDG YDCYFDNVGGEFSNTVIGQMKKFGRIAICGAISTYNRTGPLPPGPPPEIVIYQELRMEAFVVYRWQGDAR QKALKDLLKWVLEGKIQYKEYIIEGFENMPAAFMGMLKGDNLGKTIVKA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146108 |
ORF Size | 987 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146108.1, NP_001139580.1 |
RefSeq Size | 1409 bp |
RefSeq ORF | 990 bp |
Locus ID | 22949 |
UniProt ID | Q14914 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Novel involvement of miR-522-3p in high-mobility group box 1-induced prostaglandin reductase 1 expression and reduction of phagocytosis.
,null,
Biochimica et biophysica acta. Molecular cell research
,PubMed ID 28088550
[PTGR1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228825 | PTGR1 (Myc-DDK-tagged)-Human prostaglandin reductase 1 (PTGR1), transcript variant 1 |
CNY 3,600.00 |
|
RC228825L3 | Lenti ORF clone of Human prostaglandin reductase 1 (PTGR1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC228825L4 | Lenti ORF clone of Human prostaglandin reductase 1 (PTGR1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC327460 | PTGR1 (untagged)-Human prostaglandin reductase 1 (PTGR1) transcript variant 1 |
CNY 6,270.00 |