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ZNF135 (NM_007134) Human Tagged ORF Clone
CAT＃: RG228534
- TrueORF®
ZNF135 (tGFP-tagged) - Human zinc finger protein 135 (ZNF135), transcript variant 1. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_007134" in other vectors (2)
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CNY 6,370.00
货期*
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | pHZ-17; pT3; ZNF61; ZNF78L1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228534 ORF sequence, codon optimized.
Due to the complexity of NM_007134, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGGGAGTAGAAGACGCTCTGTGGGTTGCAGATGTCGGGGACTTTGCCTGGCGGTTCGCAGAG AACAGGTGACTTTTGAAGACGTCGTCGTGGGGTTTAGCCAAGAGGAGTGGGGTCAGCTTAAGCCCGCACA ACGCACACTGTACAGGGACGTAATGTTGGATACGTTCAGACTGCTCGTCTCCGTCGGTCACTGGCTGCCC AAGCCTAACGTGATCAGCCTCCTGGAGCAGGAAGCCGAGTTGTGGGCCGTGGAGTCACGACTCCCGCAGG GAGTATACCCCGAGATCAAGGGACATTTCCAGTTCCTGCTTCTGAGTGATCTGGAGACACGGCCAAAGGT AAAACTGAGCGTACTCAAGCAGGGCATCAGTGAAGAGATAAGCAACAGCGTTATCCTCGTGGAACGCTTT CTGTGGGACGGACTTTGGTATTGTCGGGGTGAAGATACGGAGGGGCATTGGGAATGGTCATGTGAGAGCC TCGAAAGTCTGGCCGTGCCGGTGGCATTTACCCCTGTGAAAACACCTGTGCTCGAACAGTGGCAGAGAAA TGGCTTCGGGGAAAATATCAGCCTCAACCCCGATCTGCCGCATCAGCCCATGACGCCAGAACGCCAGAGC CCACACACCTGGGGGACACGCGGGAAAAGAGAAAAACCAGACCTCAACGTACTGCAAAAGACATGTGTGA AAGAAAAACCCTATAAGTGCCAGGAGTGTGGAAAGGCATTCTCACACAGCTCCGCGTTGATCGAGCATCA CAGAACGCACACCGGGGAACGCCCGTACGAATGCCACGAATGTTTGAAAGGCTTTCGCAATAGCAGCGCC CTGACGAAGCACCAAAGAATCCATACAGGAGAGAAACCTTACAAATGCACACAGTGTGGCCGGACTTTTA ACCAGATAGCGCCTTTGATACAGCATCAGCGAACACATACTGGCGAGAAACCTTATGAGTGCTCTGAGTG CGGAAAGTCATTTTCATTCAGGTCATCCTTCTCACAACACGAGAGAACTCACACTGGCGAAAAGCCCTAC GAATGTAGTGAGTGCGGGAAAGCTTTTAGGCAATCTATACACCTGACCCAACACCTTAGGATCCACACTG GCGAGAAGCCTTACCAGTGCGGGGAATGTGGAAAAGCTTTCTCACACTCCTCTTCACTTACCAAGCATCA GAGGATTCACACTGGGGAAAAACCTTACGAATGTCATGAGTGCGGGAAAGCGTTCACCCAGATCACCCCT CTCATCCAGCATCAGCGGACACATACAGGCGAAAAGCCGTACGAGTGTGGAGAGTGCGGCAAAGCATTCA GCCAGTCTACACTGCTTACCGAGCACAGAAGGATTCACACCGGAGAGAAGCCATATGGGTGTAACGAATG TGGGAAAACATTCAGTCATAGTAGCTCCCTCAGCCAGCACGAACGAACTCATACCGGCGAGAAGCCCTAC GAATGCTCACAGTGCGGCAAAGCTTTCCGACAGAGCACTCATCTGACACAGCACCAGCGAATCCACACAG GCGAAAAACCATATGAATGCAACGACTGTGGCAAAGCATTTAGTCACTCCAGCAGCCTGACTAAACATCA ACGCATCCACACTGGGGAAAAACCGTATGAATGCAACCAGTGTGGTAGAGCCTTTAGTCAACTTGCACCA CTCATTCAGCACCAAAGAATTCACACAGGAGAAAAACCTTACGAATGCAATCAGTGTGGACGAGCATTCA GTCAGAGCTCTCTGCTCATAGAACATCAGCGAATACACACAAAGGAAAAACCTTACGGCTGCAATGAGTG TGGAAAAAGCTTTAGTCACTCCAGCTCACTCTCTCAGCATGAGCGGACCCACACCGGAGAGAAACCATAC GAGTGTCACGATTGTGGCAAAAGCTTCCGGCAGAGCACCCACCTGACCCAGCACCGCAGAATCCACACCG GTGAGAAACCATATGCATGTAGGGATTGCGGTAAAGCATTCACCCACTCATCCAGCCTTACTAAGCATCA AAGAACTCACACTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228534 representing NM_007134
Red=Cloning site Green=Tags(s) MELGSRRRSVGCRCRGLCLAVRREQVTFEDVVVGFSQEEWGQLKPAQRTLYRDVMLDTFRLLVSVGHWLP KPNVISLLEQEAELWAVESRLPQGVYPEIKGHFQFLLLSDLETRPKVKLSVLKQGISEEISNSVILVERF LWDGLWYCRGEDTEGHWEWSCESLESLAVPVAFTPVKTPVLEQWQRNGFGENISLNPDLPHQPMTPERQS PHTWGTRGKREKPDLNVLQKTCVKEKPYKCQECGKAFSHSSALIEHHRTHTGERPYECHECLKGFRNSSA LTKHQRIHTGEKPYKCTQCGRTFNQIAPLIQHQRTHTGEKPYECSECGKSFSFRSSFSQHERTHTGEKPY ECSECGKAFRQSIHLTQHLRIHTGEKPYQCGECGKAFSHSSSLTKHQRIHTGEKPYECHECGKAFTQITP LIQHQRTHTGEKPYECGECGKAFSQSTLLTEHRRIHTGEKPYGCNECGKTFSHSSSLSQHERTHTGEKPY ECSQCGKAFRQSTHLTQHQRIHTGEKPYECNDCGKAFSHSSSLTKHQRIHTGEKPYECNQCGRAFSQLAP LIQHQRIHTGEKPYECNQCGRAFSQSSLLIEHQRIHTKEKPYGCNECGKSFSHSSSLSQHERTHTGEKPY ECHDCGKSFRQSTHLTQHRRIHTGEKPYACRDCGKAFTHSSSLTKHQRTHTG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007134 |
| ORF Size | 2046 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007134.1, NP_009065.1 |
| RefSeq Size | 3346 bp |
| RefSeq ORF | 2049 bp |
| Locus ID | 7694 |
| UniProt ID | P52742 |
| Protein Families | Transcription Factors |
| Gene Summary | Plays a role in the regulation of cell morphology and cytoskeletal organization. May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] |
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