United States
Germany
Japan
United Kingdom
China
TLE2 (NM_001144761) Human Tagged ORF Clone
CAT＃: RG227876
- TrueORF®
TLE2 (tGFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2
ORF Plasmid: DDK
"NM_001144761" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,170.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ESG; ESG2; GRG2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227876 representing NM_001144761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAATCACGGCTCACTGCAACCTCCGCCTCCCAAGATTCTCCTGCTTCAGGATTACAGACCCCGC TCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGATCTGCGACCGCATCAAAGAAGAATTCCAGTT TCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGAGAAGCTGGCCAGCGAGAAGACGGAAATGCAG CGACATTATGTCATGTATTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGGAGATTG TGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCAGGTGCT CCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGGGCAGCAGCAGCTC CAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCCCGCCCAGCCGGGCTGGTGGGCGGCAGTG CTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCCAGGCTCAGCTGGCGGCGGCTGTCAAGGAGGA CCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAGAGCCCCGAGCAGGAGTGCATCTCCCTCGCCC CCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGTGGTGGCGGGAAGCAGAGAGCAGATGAGA AGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTGATTACAATCTGGTGGTGGACGAGGACCA ACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAAGGTACCCATCTGCATTCCTGCCCGTCGG GACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGCTCACCGCTGCCTAGAGCCAAGGAGCTCA TCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCTGTGACTCCTCCCCGCCCCAGGACGCTTC CACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGCTGCCAAGCCAGCACCTTCCACGGACAGC GTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACCACGTCCTTCAGCCTGGGCTCCCACAGCA CTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCCTCCACCTGTCCCCCCAGGTCAGCAGCTC TGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCATCCCCATCTCCGAGGGTCATCCGTCTCT TCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCCTTCCACGTGTCTGCGGACGGGCAGATGC AGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCATCCCGCGGCACGCCCGGCAGCTGCACAC GCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGGCTCCACACAGCATGTGTACACGGGCGGC AAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCCAAGACGCCCGTGGCCCAGCTCGACTGCC TGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGGATGGCCGGAGTCTGATCGTGGGCGGTGA GGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCCCCGTATCAAGGCCGAGCTGACTTCCTCA GCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAGGTTTGCTTCTCCTGCTGCAGCGATGGCA ACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGCAGTTCCAGGGCCACACGGACGGCGCCAG CTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGGGGGCCTGGACAACACGGTGCGCTGCTGG GACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGCTCCCAGATTTTCTCCCTGGGCCACTGCC CTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACGTGGAGATCCTGCACGTCCGCAAGCCGGA GAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCTGAAGTTTGCCTCCTGCGTCCAAGGAGTC GTCCTCAGTCCTGAGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227876 representing NM_001144761
Red=Cloning site Green=Tags(s) MVQSRLTATSASQDSPASGLQTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQ RHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQL QPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSP PESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARR DLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDS VALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVS SSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGG KGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSS APACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCW DLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCVQGV VLSPEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144761 |
| ORF Size | 2118 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144761.1, NP_001138233.1 |
| RefSeq Size | 2508 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 7089 |
| UniProt ID | Q04725 |
| Protein Families | Transcription Factors |
| Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227876 | TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2 |
CNY 7,410.00 |
|
| RC227876L3 | Lenti-ORF clone of TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2 |
CNY 9,310.00 |
|
| RC227876L4 | Lenti-ORF clone of TLE2 (mGFP-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2 |
CNY 9,310.00 |
|
| SC326190 | TLE2 (untagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2 |
CNY 13,590.00 |
