LANPL (ANP32E) (NM_001136478) Human Tagged ORF Clone

CAT#: RG227678

  • TrueORF®

ANP32E (tGFP-tagged) - Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member E (ANP32E), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001136478" in other vectors (2)

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CNY 4,370.00


货期*
详询

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-ANP32E Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms LANP-L; LANPL
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG227678 representing NM_001136478
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATGAAGAAGAAGATTAACCTGGAGTTAAGGAACAGATCCCCGGAGGAGGTGACAGAGTTAGTCC
TTGATAATTGCCTGTGTGTCAATGGGGAAATTGAAGGCCTGAATGATACTTTCAAAGAACTAGAATTTCT
GAGTATGGCTAATGTGGAACTAAGTTCGCTGGCCCGGCTTCCCAGCTTAAATAAACTTCGAAAACAAAAT
CTTAAAAATTTGAAAAGTCTTGACCTGTTTAACTGTGAGATCACAAACCTGGAAGATTATAGAGAAAGTA
TTTTTGAACTACTGCAGCAAATCACATACTTAGATGGATTTGATCAGGAGGATAATGAAGCGCCGGACTC
TGAAGAGGAGGATGATGAGGATGGCGATGAAGATGATGAAGAGGAAGAGGAAAATGAAGCTGGTCCACCG
GAAGGATATGAGGAAGAGGAGGAGGAAGAGGAAGAGGAGGATGAGGATGAGGATGAAGATGAAGATGAAG
CAGGTTCAGAGTTGGGAGAGGGAGAAGAGGAAGTGGGCCTCTCATACTTAATGAAAGAAGAAATTCAGGA
TGAAGAAGATGATGATGACTATGTTGAAGAAGGGGAAGAAGAGGAAGAAGAGGAAGAAGGAGGTCTTCGA
GGGGAGAAGAGGAAACGAGATGCTGAAGACGATGGAGAGGAAGAAGATGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG227678 representing NM_001136478
Red=Cloning site Green=Tags(s)

MEMKKKINLELRNRSPEEVTELVLDNCLCVNGEIEGLNDTFKELEFLSMANVELSSLARLPSLNKLRKQN
LKNLKSLDLFNCEITNLEDYRESIFELLQQITYLDGFDQEDNEAPDSEEEDDEDGDEDDEEEEENEAGPP
EGYEEEEEEEEEEDEDEDEDEDEAGSELGEGEEEVGLSYLMKEEIQDEEDDDDYVEEGEEEEEEEEGGLR
GEKRKRDAEDDGEEEDD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001136478
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001136478.4
RefSeq Size 3347 bp
RefSeq ORF 684 bp
Locus ID 81611
UniProt ID Q9BTT0
Protein Families Druggable Genome
Gene Summary Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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