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CAPN8 (NM_001143962) Human Tagged ORF Clone
CAT＃: RG227531
- TrueORF®
CAPN8 (tGFP-tagged) - Human calpain 8 (CAPN8)
ORF Plasmid: DDK
"NM_001143962" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | nCL-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227531 representing NM_001143962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCAGGCAGCTGGTGTATCTAGGCAGCGGGCAGCCACTCAAGGTCTTGGCTCCAACCAAAACG CTTTGAAGTACTTGGGCCAGGATTTCAAGACCCTGAGGCAACAGTGCTTGGACTCAGGGGTCCTATTTAA GGACCCTGAGTTCCCAGCATGTCCATCAGCTTTGGGCTACAAGGATCTTGGACCAGGCTCTCCGCAAACT CAAGGCATCATCTGGAAGCGGCCCACGGAGTTGTGTCCCAGCCCTCAGTTTATCGTTGGTGGAGCCACGC GCACAGACATTTGTCAGGGTGGTCTAGGTGACTGCTGGCTTCTGGCTGCCATTGCCTCCCTGACCCTGAA TGAAGAGCTGCTTTACCGGGTGGTCCCCAGGGACCAGGACTTCCAGGAGAACTATGCGGGAATCTTTCAC TTTCAGTTCTGGCAGTACGGAGAGTGGGTGGAGGTGGTCATTGACGACAGGCTGCCCACCAAGAATGGAC AGCTGCTCTTCCTACACTCGGAACAAGGCAATGAATTCTGGAGTGCCCTGCTGGAGAAAGCCTATGCCAA GCTTAATGGTTGTTATGAGGCTCTCGCTGGAGGTTCCACAGTGGAGGGGTTTGAGGATTTCACAGGTGGC ATCTCTGAGTTTTATGACCTGAAGAAACCACCAGCCAATCTATATCAGATCATCCGGAAGGCCCTCTGTG CGGGGTCTCTGCTGGGCTGCTCCATTGATGTCTCCAGTGCAGCCGAAGCCGAAGCCATCACCAGCCAGAA GCTGGTTAAGAGTCATGCGTACTCTGTCACTGGAGTCGAAGAGGTGAATTTCCAGGGCCATCCAGAGAAG CTGATCAGACTCAGGAATCCATGGGGTGAAGTGGAGTGGTCGGGAGCCTGGAGCGATGATGCACCAGAGT GGAATCACATAGACCCCCGGCGGAAGGAAGAACTGGACAAGAAAGTTGAGGATGGAGAATTCTGGATGTC ACTTTCAGATTTCGTGAGGCAGTTCTCTCGGTTGGAGATCTGCAACCTGTCCCCGGACTCTCTGAGTAGC GAGGAGGTGCACAAATGGAACCTGGTCCTGTTCAACGGCCACTGGACCCGGGGCTCCACAGCTGGGGGCT GCCAGAACTACCCAGCCACGTACTGGACCAATCCCCAGTTCAAAATCCGTTTGGATGAAGTGGATGAGGA CCAGGAGGAGAGCATCGGTGAACCCTGCTGTACAGTGCTGCTGGGCCTGATGCAGAAAAATCGCAGGTGG CGGAAGCGGATAGGACAAGGCATGCTTAGCATCGGCTATGCCGTCTACCAGGTTCCCAAGGAGCTGGAGA GTCACACGGACGCACACTTGGGCCGGGATTTCTTCCTGGCCTACCAGCCCTCAGCCCGCACCAGCACCTA CGTCAACCTGCGGGAGGTCTCTGGCCGGGCCCGGCTGCCCCCTGGGGAGTACCTGGTGGTGCCATCCACA TTTGAACCCTTCAAAGACGGCGAGTTCTGCTTGAGAGTGTTCTCAGAGAAGAAGGCCCAGGCCCTAGAAA TTGGGGATGTGGTAGCTGGAAACCCATATGAGCCACATCCCAGTGAGGTGGATCAGGAAGATGACCAGTT CAGGAGGCTGTTTGAGAAGTTGGCAGGGAAGGATTCTGAGATTACTGCCAATGCACTCAAGATACTTTTG AATGAGGCGTTTTCCAAGAGAACAGACATAAAATTCGATGGATTCAACATCAACACTTGCAGGGAAATGA TCAGTCTGTTGGATAGCAATGGAACGGGCACTTTGGGGGCGGTGGAATTCAAGACGCTCTGGCTGAAGAT TCAGAAGTATCTGGAGATCTATTGGGAAACTGATTATAACCACTCGGGCACCATCGATGCCCACGAGATG AGGACAGCCCTCAGGAAGGCAGGTTTCACCCTCAACAGCCAGGTGCAGCAGACCATTGCCCTGCGGTATG CGTGCAGCAAGCTCGGCATCAACTTTGACAGCTTCGTGGCTTGTATGATCCGCCTGGAGACCCTCTTCAA ACTATTCAGCCTTCTGGACGAAGACAAGGATGGCATGGTTCAGCTCTCTCTGGCCGAGTGGCTGTGCTGC GTGTTGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227531 representing NM_001143962
Red=Cloning site Green=Tags(s) MAAQAAGVSRQRAATQGLGSNQNALKYLGQDFKTLRQQCLDSGVLFKDPEFPACPSALGYKDLGPGSPQT QGIIWKRPTELCPSPQFIVGGATRTDICQGGLGDCWLLAAIASLTLNEELLYRVVPRDQDFQENYAGIFH FQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEQGNEFWSALLEKAYAKLNGCYEALAGGSTVEGFEDFTGG ISEFYDLKKPPANLYQIIRKALCAGSLLGCSIDVSSAAEAEAITSQKLVKSHAYSVTGVEEVNFQGHPEK LIRLRNPWGEVEWSGAWSDDAPEWNHIDPRRKEELDKKVEDGEFWMSLSDFVRQFSRLEICNLSPDSLSS EEVHKWNLVLFNGHWTRGSTAGGCQNYPATYWTNPQFKIRLDEVDEDQEESIGEPCCTVLLGLMQKNRRW RKRIGQGMLSIGYAVYQVPKELESHTDAHLGRDFFLAYQPSARTSTYVNLREVSGRARLPPGEYLVVPST FEPFKDGEFCLRVFSEKKAQALEIGDVVAGNPYEPHPSEVDQEDDQFRRLFEKLAGKDSEITANALKILL NEAFSKRTDIKFDGFNINTCREMISLLDSNGTGTLGAVEFKTLWLKIQKYLEIYWETDYNHSGTIDAHEM RTALRKAGFTLNSQVQQTIALRYACSKLGINFDSFVACMIRLETLFKLFSLLDEDKDGMVQLSLAEWLCC VLV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143962 |
| ORF Size | 2109 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143962.1, NP_001137434.1 |
| RefSeq Size | 2427 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 388743 |
| UniProt ID | A6NHC0 |
| Protein Families | Druggable Genome |
| Gene Summary | Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227531 | CAPN8 (Myc-DDK-tagged)-Human calpain 8 (CAPN8) |
CNY 5,152.00 |
|
| RC227531L3 | Lenti-ORF clone of CAPN8 (Myc-DDK-tagged)-Human calpain 8 (CAPN8) |
CNY 5,890.00 |
|
| RC227531L4 | Lenti-ORF clone of CAPN8 (mGFP-tagged)-Human calpain 8 (CAPN8) |
CNY 5,890.00 |
|
| SC325288 | CAPN8 (untagged)-Human calpain 8 (CAPN8) |
CNY 7,220.00 |
