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SORBS2 (NM_001145674) Human Tagged ORF Clone
CAT＃: RG227308
- TrueORF®
SORBS2 (tGFP-tagged) - Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 7
ORF Plasmid: DDK
"NM_001145674" in other vectors (4)
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CNY 11,020.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARGBP2; PRO0618 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227308 representing NM_001145674
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCAACAACACCTTTGCAGACAGTCGACCGGCCCAAGGACTGGTACAAGACGATGTTTAAGCAAA TTCACATGGTGCACAAGCCGGGTCTGTACAACCCACCCTACAGTGCTCAGTCACACCCTGCTGCAAAGAC CCAAACCTACAGACCTCTTTCCAAAAGCCACTCCGACAACAGCCCCAATGCCTTTAAGGATGCGTCCTCC CCAGTGCCTCCCCCACATGTTCCACCTCCAGTCCCGCCGCTTCGACCAAGAGATCGGTCTTCAACAGAAA AGCATGACTGGGATCCTCCAGACAGAAAAGTGGACACAAGAAAATTTCGGTCTGAGCCAAGGAGTATTTT TGAATATGAACCTGGCAAGTCATCAATTCTTCAGCATGAAAGACCAGCCTCCTTGTATCAGTCCTCTATA GACAGAAGCCTGGAAAGACCCATGAGTTCTGCAAGCATGGCCAGTGACTTCAGGAAGCGGAGGAAGAGCG AGCCTGCAGTGGGTCCACCACGGGGCTTGGGAGATCAAAGTGCGAGCAGGACTAGCCCAGGCCGAGTGGA CCTCCCAGGATCAAGCACCACTCTTACAAAGTCTTTCACTAGCTCTTCTCCTTCTTCCCCATCAAGAGCA AAAGGTGGGGATGATAGCAAAATATGTCCATCCCTTTGCAGTTACTCAGGGCTCAACGGCAACCCCTCCA GTGAGTTAGATTACTGTAGCACTTACAGACAGCACTTGGATGTCCCTCGGGACTCACCAAGAGCCATTAG TTTCAAGAACGGCTGGCAAATGGCCCGGCAAAACGCAGAAATCTGGAGCAGCACGGAAGAAACCGTCTCT CCCAAAATCAAATCCCGGAGCTGTGACGATCTCCTAAACGACGACTGCGATAGCTTCCCGGACCCGAAAG TCAAGTCGGAAAGCATGGGCTCCCTGTTATGTGAGGAGGACTCCAAGGAGAGCTGCCCCATGGCGTGGGG GTCCCCCTACGTCCCGGAGGTTCGCAGTAACGGCAGATCAAGGATCAGACACAGGTCAGCCCGCAACGCC CCGGGGTTCCTGAAGATGTACAAGAAAATGCACCGCATCAACCGCAAGGACCTGATGAACTCCGAGGTGA TCTGCTCCGTGAAGTCCAGGATTCTGCAGTACGAGAGCGAGCAGCAGCACAAGGACCTGCTGCGCGCCTG GAGCCAGTGCTCCACGGAGGAGGTGCCCCGGGACATGGTGCCCACACGCATCTCCGAATTCGAAAAGCTG ATCCAGAAGTCCAAATCCATGCCAAATTTAGGGGATGACATGCTGTCTCCCGTAACCCTAGAACCACCGC AAAATGGCCTATGTCCCAAGAGGCGGTTTTCCATTGAGTATTTGCTGGAGGAAGAAAATCAAAGCGGCCC CCCCGCTCGGGGCCGGCGAGGCTGCCAGTCTAACGCCCTGGTGCCCATTCACATTGAAGTCACCAGCGAT GAGCAGCCCCGAGCACACGTGGAGTTTTCCGACAGCGACCAGGACGGGGTTGTGTCCGACCACAGTGACT ACATTCACCTAGAGGGGTCATCCTTCTGCAGTGAAAGTGACTTTGATCACTTTTCCTTCACATCCTCCGA AAGCTTTTACGGATCCAGCCACCACCACCACCATCACCACCACCACCACCACCGCCACCTCATCAGCTCC TGCAAAGGCAGGTGCCCGGCCTCCTACACTCGATTTACCACAATGTTAAAACACGAAAGAGCCAGACACG AAAACACCGAGGAGCCCAGAAGGCAAGAAATGGACCCTGGCCTTTCTAAACTTGCTTTTCTAGTCAGTCC TGTGCCTTTCCGGAGGAAAAAAAATTCGGCTCCTAAGAAACAGACTGAAAAGGCAAAATGTAAAGCATCT GTGTTTGAGGCTCTGGACTCTGCCCTTAAAGACATCTGTGACCAAATTAAAGCTGAAAAGAAAAGGGGGA GCTTGCCGGACAACAGCATCCTGCACCGCCTCATCAGTGAGCTGCTGCCAGATGTTCCCGAGAGGAACTC ATCCCTGAGAGCGCTGAGGAGGAGCCCCCTGCACCAGCCTCTCCACCCACTGCCTCCCGATGGTGCTATT CATTGTCCACCCTACCAGAATGACTGCGGGAGAATGCCCCGCAGTGCCTCTTTCCAAGACGTGGACACAG CCAACAGCAGCTGCCACCACCAAGACCGTGGCGGTGCACTCCAAGACCGTGAGTCCCCTAGAAGTTACTC ATCCACTTTGACTGACATGGGGAGAAGTGCACCAAGGGAAAGAAGAGGAACTCCAGAAAAAGAGAAATTG CCTGCAAAAGCTGTTTATGATTTTAAGGCTCAGACATCTAAGGAGTTGTCATTTAAGAAAGGAGATACTG TCTACATCCTCAGGAAAATTGATCAAAATTGGTATGAGGGAGAACACCACGGGAGAGTGGGCATCTTCCC GATCTCATACGTAGAGAAACTCACACCTCCTGAGAAAGCACAGCCTGCAAGACCACCTCCGCCAGCCCAG CCCGGAGAAATCGGAGAAGCTATAGCCAAATACAACTTCAACGCAGACACAAATGTGGAGCTGTCACTGA GAAAGGGAGATAGAGTTATTCTTCTTAAAAGAGTTGATCAAAACTGGTATGAAGGTAAAATCCCAGGAAC CAACAGACAAGGCATCTTCCCTGTTTCCTATGTGGAGGTCGTCAAGAAGAACACAAAAGGTGCTGAGGAC TACCCTGACCCTCCAATACCCCACAGCTATTCTAGTGATAGGATTCACAGCTTGAGCTCAAATAAGCCAC AGCGTCCTGTGTTTACTCATGAAAATATTCAAGGTGGGGGGGAACCGTTTCAGGCTCTGTATAACTATAC TCCCAGGAATGAAGATGAGCTGGAGCTCAGAGAAAGTGATGTCATTGATGTCATGGAAAAGTGTGATGAC GGCTGGTTTGTGGGGACCTCAAGAAGAACCAAATTCTTTGGTACTTTCCCCGGAAACTACGTCAAGAGGC TG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227308 representing NM_001145674
Red=Cloning site Green=Tags(s) MKATTPLQTVDRPKDWYKTMFKQIHMVHKPGLYNPPYSAQSHPAAKTQTYRPLSKSHSDNSPNAFKDASS PVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPASLYQSSI DRSLERPMSSASMASDFRKRRKSEPAVGPPRGLGDQSASRTSPGRVDLPGSSTTLTKSFTSSSPSSPSRA KGGDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPRAISFKNGWQMARQNAEIWSSTEETVS PKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPMAWGSPYVPEVRSNGRSRIRHRSARNA PGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYESEQQHKDLLRAWSQCSTEEVPRDMVPTRISEFEKL IQKSKSMPNLGDDMLSPVTLEPPQNGLCPKRRFSIEYLLEEENQSGPPARGRRGCQSNALVPIHIEVTSD EQPRAHVEFSDSDQDGVVSDHSDYIHLEGSSFCSESDFDHFSFTSSESFYGSSHHHHHHHHHHHRHLISS CKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFLVSPVPFRRKKNSAPKKQTEKAKCKAS VFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPERNSSLRALRRSPLHQPLHPLPPDGAI HCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRGGALQDRESPRSYSSTLTDMGRSAPRERRGTPEKEKL PAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPAQ PGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKKNTKGAED YPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELELRESDVIDVMEKCDD GWFVGTSRRTKFFGTFPGNYVKRL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145674 |
| ORF Size | 3012 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145674.1, NP_001139146.1 |
| RefSeq Size | 5529 bp |
| RefSeq ORF | 3015 bp |
| Locus ID | 8470 |
| UniProt ID | O94875 |
| Gene Summary | Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227308 | SORBS2 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 7 |
CNY 9,980.00 |
|
| RC227308L3 | Lenti-ORF clone of SORBS2 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 7 |
CNY 11,880.00 |
|
| RC227308L4 | Lenti-ORF clone of SORBS2 (mGFP-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 7 |
CNY 11,880.00 |
|
| SC326636 | SORBS2 (untagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 7, mRNA |
CNY 19,290.00 |
