RNF156 (MGRN1) (NM_001142289) Human Tagged ORF Clone
CAT#: RG227266
- TrueORF®
MGRN1 (tGFP-tagged) - Human mahogunin, ring finger 1 (MGRN1), transcript variant 2
ORF Plasmid: DDK
"NM_001142289" in other vectors (4)
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CNY 5,230.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | RNF156 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227266 representing NM_001142289
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTATC GCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACCCC CCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAGTTT CCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAAGACT CCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGGGTGCT CTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCATCGGAG GAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTCCACTACA AGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAGGATGACGA GCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGAGATGCA GTGGTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGCAGCTTCT CTGTGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATCTATGGCAT TGAGAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAACGAGTGTGTG GTGTGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGTACCTCCTGCG CCGACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCCCTCCTGCAGAT CCGGGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTGGCCCAGAGCCTG GAGCATGATGAGCACTCTAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGC TCAACGGCCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGG CATCTCGGACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATCCTGGACAGCAGCCGC CAGAAGGGCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCTTCCCCCATCCACGAAG AGGATGAGGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGCGCAGAGCTGGCCCTGCG GGAAAGCAGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCGTCACCACAGCAAGGGACC CGAGCAGCTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGTGGCCGAGGCCCACCTGCTG ACATCTACCTGCCAGGACGGCCCACCTCCATGGAGACGGCCCACGGCCTCGCCACCACCAGCCCCACCTG GCCTCCACTTGGTGGCCCCAGCCCCGATCCCAGCGCCGCCGAGCTGACCCCACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227266 representing NM_001142289
Red=Cloning site Green=Tags(s) MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASE EFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDA VVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECV VCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSL EHDEHSNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSR QKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGT RAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAHGLATTSPTWPPLGGPSPDPSAAELTPL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142289 |
ORF Size | 1665 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142289.1, NP_001135761.1 |
RefSeq Size | 3870 bp |
RefSeq ORF | 1665 bp |
Locus ID | 23295 |
UniProt ID | O60291 |
Protein Pathways | Ubiquitin mediated proteolysis |
Gene Summary | Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227266 | MGRN1 (Myc-DDK-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 2 |
CNY 3,656.00 |
|
RC227266L3 | Lenti-ORF clone of MGRN1 (Myc-DDK-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 2 |
CNY 6,650.00 |
|
RC227266L4 | Lenti-ORF clone of MGRN1 (mGFP-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 2 |
CNY 6,650.00 |
|
SC326066 | MGRN1 (untagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 2 |
CNY 8,840.00 |