PRRG1 (NM_001142395) Human Tagged ORF Clone
CAT#: RG226935
- TrueORF®
PRRG1 (tGFP-tagged) - Human proline rich Gla (G-carboxyglutamic acid) 1 (PRRG1), transcript variant 2
ORF Plasmid: DDK
"NM_001142395" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PRGP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226935 representing NM_001142395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGGGTTTTCCTCACGGGAGAAAAAGCCAATTCCATATTAAAACGCTACCCAAGAGCTAATGGGT TTTTTGAAGAAATAAGACAGGGCAACATTGAGCGTGAGTGCAAAGAAGAATTCTGTACATTTGAAGAAGC AAGAGAAGCTTTTGAAAATAATGAAAAAACTAAGGAGTTTTGGAGCACCTACACAAAAGCGCAACAAGGG GAGAGTAACCGAGGAAGTGACTGGTTTCAGTTTTACCTTACCTTTCCGTTAATCTTTGGCCTCTTCATTA TCCTCCTTGTCATTTTCCTAATCTGGAGATGCTTCCTAAGAAACAAAACTCGTAGACAGACAGTGACTGA AGGCCACATTCCTTTCCCTCAGCACCTTAATATTATCACCCCACCCCCCCCACCAGATGAAGTGTTTGAC AGCAGTGGATTGTCTCCAGGCTTTCTGGGATATGTAGTTGGGCGCTCAGATTCCGTCTCTACTCGCCTGT CCAATTGTGATCCCCCGCCAACCTATGAGGAAGCCACTGGCCAAGTGAACCTGCAGAGGAGTGAAACAGA ACCTCATTTAGACCCACCCCCAGAGTATGAGGACATAGTCAACTCCAACTCAGCCAGTGCCATTCCTATG GTGCCTGTGGTCACCACCATCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226935 representing NM_001142395
Red=Cloning site Green=Tags(s) MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQG ESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFD SSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPM VPVVTTIK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001142395 |
ORF Size | 654 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142395.2 |
RefSeq Size | 4508 bp |
RefSeq ORF | 657 bp |
Locus ID | 5638 |
UniProt ID | O14668 |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a vitamin K-dependent, gamma-carboxyglutamic acid (Gla)-containing, single-pass transmembrane protein. This protein contains a Gla domain at the N-terminus, preceded by a propeptide sequence required for post-translational gamma-carboxylation of specific glutamic acid residues by a vitamin K-dependent gamma-carboxylase. The C-terminus is proline-rich containing PPXY and PXXP motifs found in a variety of signaling and cytoskeletal proteins. This gene is highly expressed in the spinal cord. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226935 | PRRG1 (Myc-DDK-tagged)-Human proline rich Gla (G-carboxyglutamic acid) 1 (PRRG1), transcript variant 2 |
CNY 2,400.00 |
|
RC226935L3 | Lenti ORF clone of Human proline rich Gla (G-carboxyglutamic acid) 1 (PRRG1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC226935L4 | Lenti ORF clone of Human proline rich Gla (G-carboxyglutamic acid) 1 (PRRG1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC324796 | PRRG1 (untagged)-Human proline rich Gla (G-carboxyglutamic acid) 1 (PRRG1), transcript variant 2 |
CNY 3,990.00 |