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TLK1 (NM_001136555) Human Tagged ORF Clone
CAT＃: RG226651
- TrueORF®
TLK1 (tGFP-tagged) - Human tousled-like kinase 1 (TLK1), transcript variant 3
ORF Plasmid: DDK
"NM_001136555" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PKU-beta |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226651 representing NM_001136555
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTTTTATTCTTGTATGATTTGAAAACTACTGGCAAGACACCGGAGAAGAAACAATCGGAATCAT CCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGTGG CCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCT GCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGCCCTT CTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATTATTCA GACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAGAAGGAG GGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACAAAAATTAC TTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAGAGTAC ACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTCGGGCACTTTACAACAGTT AGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAAGCAACAAG AATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGCAAACCTCCCAC AGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAAGCAGTCAATGGA GCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAATATCATGAACAGG AAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAACTTGAACG TTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATTCACAGTTC AAAGATCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTTTAGTGAAG TGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAGCTTAATAAAAGCTG GAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGAATACACAAAGAACTGGAT CACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACGTTTTGTACAGTGTTAGAAT ACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAAGCTCGGTC TATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATACATTATGAT CTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGATTTTGGTC TGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCCCAGGGGGCAGGCAC TTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCACCAAAGATTTCCAACAAGGTTGAT GTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGAAAGCCATTTGGTCACAATCAATCTC AACAAGACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTTGT AAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTGATGTGCAC CAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCTACACATGG CTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226651 representing NM_001136555
Red=Cloning site Green=Tags(s) MAVLFLYDLKTTGKTPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPP AIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKE GRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTV RHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNG AENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQF KDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELD HPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYD LKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVD VWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVH QLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136555 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136555.2 |
| RefSeq Size | 5081 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 9874 |
| UniProt ID | Q9UKI8 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226651 | TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 4,912.00 |
|
| RC226651L3 | Lenti-ORF clone of TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 9,030.00 |
|
| RC226651L4 | Lenti-ORF clone of TLK1 (mGFP-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 9,030.00 |
|
| SC326160 | TLK1 (untagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 13,020.00 |
