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DSN1 (NM_001145317) Human Tagged ORF Clone
CAT＃: RG226634
- TrueORF®
DSN1 (tGFP-tagged) - Human DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) (DSN1), transcript variant 4
ORF Plasmid: DDK
"NM_001145317" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226634 representing NM_001145317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCAGTGACTAGATCAGAGATCATAGATGAGCTCAGCCGGTCTATCAGTGTCGATTTAGCAGAAA GCAAACGGCTTGGCTGTCTCCTGCTTTCCAGTTTCCAGTTCTCTATTCAGAAACTTGAACCTTTCCTAAG GGACACTAAGGGCTTCAGTCTTGAAAGTTTTAGAGCCAAAGCATCTTCTCTTTCTGAAGAATTGAAACAT TTTGCAGACGGACTGGAAACTGATGGAACTCTACAAAAATGTTTTGAAGATTCAAATGGAAAAGCATCAG ATTTTTCTTTGGAAGCATCTGTGGCTGAGATGAAGGAATACATAACAAAGTTTTCTTTAGAACGTCAGAC TTGGGATCAGCTCTTGCTTCACTACCAGCAGGAGGCTAAAGAGATATTGTCCAGAGGATCAACTGAGGCC AAAATTACTGAGGTCAAAGTGGAACCTATGACATATCTTGGGTCTTCTCAGAATGAAGTTCTTAATACAA AACCTGACTACCAGAAAATATTACAGAACCAGAGCAAAGTCTTTGACTGTATGGAGTTGGTGATGGATGA ACTGCAAGGATCAGTGAAACAGCTGCAGGCCTTTATGGATGAAAGTACCCAGTGCTTCCAGAAGGTGTCA GTACAGCTCGGAAAGAGAAGCATGCAACAATTAGATCCCTCACCAGCTCGAAAACTGTTGAAGCTTCAGC TACAGAACCCACCTGCCATACATGGATCTGGATCTGGATCTTGTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226634 representing NM_001145317
Red=Cloning site Green=Tags(s) MTSVTRSEIIDELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFSLESFRAKASSLSEELKH FADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKFSLERQTWDQLLLHYQQEAKEILSRGSTEA KITEVKVEPMTYLGSSQNEVLNTKPDYQKILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVS VQLGKRSMQQLDPSPARKLLKLQLQNPPAIHGSGSGSCQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145317 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145317.2 |
| RefSeq Size | 1928 bp |
| RefSeq ORF | 750 bp |
| Locus ID | 79980 |
| UniProt ID | Q9H410 |
| Gene Summary | This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226634 | DSN1 (Myc-DDK-tagged)-Human DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) (DSN1), transcript variant 4 |
CNY 3,990.00 |
|
| RC226634L3 | Lenti-ORF clone of DSN1 (Myc-DDK-tagged)-Human DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) (DSN1), transcript variant 4 |
CNY 5,890.00 |
|
| RC226634L4 | Lenti-ORF clone of DSN1 (mGFP-tagged)-Human DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) (DSN1), transcript variant 4 |
CNY 5,890.00 |
|
| SC325663 | DSN1 (untagged)-Human DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) (DSN1), transcript variant 4 |
CNY 3,990.00 |
