United States
Germany
Japan
United Kingdom
China
PDE4A (NM_001111309) Human Tagged ORF Clone
CAT＃: RG226195
- TrueORF®
PDE4A (tGFP-tagged) - Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 3
ORF Plasmid: DDK
"NM_001111309" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DPDE2; PDE4; PDE46 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226195 representing NM_001111309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCCGGTGCAGCGCCCCGGGCCCGGCCCCGGCCCCCTGCCCTGGCACTGCCCCCCACGGGCCCCG AGTCCCTGACCCACTTCCCCTTCAGCGATGAGGACACCCGTCGGCACCCTCCGGGCAGATCTGTCAGCTT CGAGGCAGAGAATGGGCCGACACCATCTCCTGGCCGCAGCCCCCTGGACTCGCAGGCGAGCCCAGGACTC GTGCTGCACGCCGGGGCGGCCACCAGCCAGCGCCGGGAGTCCTTCCTGTACCGCTCAGACAGCGACTATG ACATGTCACCCAAGACCATGTCCCGGAACTCATCGGTCACCAGCGAGGCGCACGCTGAAGACCTCATCGT AACACCATTTGCTCAGGTGCTGGCCAGCCTCCGGAGCGTCCGTAGCAACTTCTCACTCCTGACCAATGTG CCCGTTCCCAGTAACAAGCGGTCCCCGCTGGGCGGCCCCACCCCTGTCTGCAAGGCCACGCTGTCAGAAG AAACGTGTCAGCAGTTGGCCCGGGAGACTCTGGAGGAGCTGGACTGGTGTCTGGAGCAGCTGGAGACCAT GCAGACCTATCGCTCTGTCAGCGAGATGGCCTCGCACAAGTTCAAAAGGATGTTGAACCGTGAGCTCACA CACCTGTCAGAAATGAGCAGGTCCGGAAACCAGGTCTCAGAGTACATTTCCACAACATTCCTGGACAAAC AGAATGAAGTGGAGATCCCATCACCCACGATGAAGGAACGAGAAAAACAGCAAGCGCCGCGACCAAGACC CTCCCAGCCGCCCCCGCCCCCTGTACCACACTTACAGCCCATGTCCCAAATCACAGGGTTGAAAAAGTTG ATGCATAGTAACAGCCTGAACAACTCTAACATTCCCCGATTTGGGGTGAAGACCGATCAAGAAGAGCTCC TGGCCCAAGAACTGGAGAACCTGAACAAGTGGGGCCTGAACATCTTTTGCGTGTCGGATTACGCTGGAGG CCGCTCACTCACCTGCATCATGTACATGATATTCCAGGAGCGGGACCTGCTGAAGAAATTCCGCATCCCT GTGGACACGATGGTGACATACATGCTGACGCTGGAGGATCACTACCACGCTGACGTGGCCTACCATAACA GCCTGCACGCAGCTGACGTGCTGCAGTCCACCCACGTACTGCTGGCCACGCCTGCACTAGATGCAGTGTT CACGGACCTGGAGATTCTCGCCGCCCTCTTCGCGGCTGCCATCCACGATGTGGATCACCCTGGGGTCTCC AACCAGTTCCTCATCAACACCAATTCGGAGCTGGCGCTCATGTACAACGATGAGTCGGTGCTCGAGAATC ACCACCTGGCCGTGGGCTTCAAGCTGCTGCAGGAGGACAACTGCGACATCTTCCAGAACCTCAGCAAGCG CCAGCGGCAGAGCCTACGCAAGATGGTCATCGACATGGTGCTGGCCACGGACATGTCCAAGCACATGACC CTCCTGGCTGACCTGAAGACCATGGTGGAGACCAAGAAAGTGACCAGCTCAGGGGTCCTCCTGCTAGATA ACTACTCCGACCGCATCCAGGTCCTCCGGAACATGGTGCACTGTGCCGACCTCAGCAACCCCACCAAGCC GCTGGAGCTGTACCGCCAGTGGACAGACCGCATCATGGCCGAGTTCTTCCAGCAGGGTGACCGAGAGCGC GAGCGTGGCATGGAAATCAGCCCCATGTGTGACAAGCACACTGCCTCCGTGGAGAAGTCTCAGGTGGGTT TTATTGACTACATTGTGCACCCATTGTGGGAGACCTGGGCGGACCTTGTCCACCCAGATGCCCAGGAGAT CTTGGACACTTTGGAGGACAACCGGGACTGGTACTACAGCGCCATCCGGCAGAGCCCATCTCCGCCACCC GAGGAGGAGTCAAGGGGGCCAGGCCACCCACCCCTGCCTGACAAGTTCCAGTTTGAGCTGACGCTGGAGG AGGAAGAGGAGGAAGAAATATCAATGGCCCAGATACCGTGCACAGCCCAAGAGGCATTGACTGCGCAGGG ATTGTCAGGAGTCGAGGAAGCTCTGGATGCAACCATAGCCTGGGAGGCATCCCCGGCCCAGGAGTCGTTG GAAGTTATGGCACAGGAAGCATCCCTGGAGGCCGAGCTGGAGGCAGTGTATTTGACACAGCAGGCACAGT CCACAGGCAGTGCACCTGTGGCTCCGGATGAGTTCTCGTCCCGGGAGGAATTCGTGGTTGCTGTAAGCCA CAGCAGCCCCTCTGCCCTGGCTCTTCAAAGCCCCCTTCTCCCTGCTTGGAGGACCCTGTCTGTTTCAGAG CATGCCCCGGGCCTCCCGGGCCTCCCCTCCACGGCGGCCGAGGTGGAGGCCCAACGAGAGCACCAGGCTG CCAAGAGGGCTTGCAGTGCCTGCGCAGGGACATTTGGGGAGGACACATCCGCACTCCCAGCTCCTGGTGG CGGGGGGTCAGGTGGAGACCCTACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226195 representing NM_001111309
Red=Cloning site Green=Tags(s) MRSGAAPRARPRPPALALPPTGPESLTHFPFSDEDTRRHPPGRSVSFEAENGPTPSPGRSPLDSQASPGL VLHAGAATSQRRESFLYRSDSDYDMSPKTMSRNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNV PVPSNKRSPLGGPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELT HLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKL MHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIP VDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVS NQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMT LLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRER ERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPP EEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESL EVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSE HAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111309 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111309.1, NP_001104779.1 |
| RefSeq Size | 4503 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 5141 |
| UniProt ID | P27815 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226195 | PDE4A (Myc-DDK-tagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 3 |
CNY 5,800.00 |
|
| RC226195L3 | Lenti-ORF clone of PDE4A (Myc-DDK-tagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 3 |
CNY 7,700.00 |
|
| RC226195L4 | Lenti-ORF clone of PDE4A (mGFP-tagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 3 |
CNY 7,700.00 |
|
| SC318989 | PDE4A (untagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 3 |
CNY 15,870.00 |
