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PGC1 beta (PPARGC1B) (NM_133263) Human Tagged ORF Clone
CAT＃: RG224480
- TrueORF®
PPARGC1B (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1
ORF Plasmid: DDK
"NM_133263" in other vectors (6)
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CNY 11,210.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ERRL1; PERC; PGC-1(beta); PGC1B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224480 representing NM_133263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGAACGACTGCGGCGCGCTGCTGGACGAAGAGCTCTCCTCCTTCTTCCTCAACTATCTCGCTG ACACGCAGGGTGGAGGGTCCGGGGAGGAGCAACTCTATGCTGACTTTCCAGAACTTGACCTCTCCCAGCT GGATGCCAGCGACTTTGACTCGGCCACCTGCTTTGGGGAGCTGCAGTGGTGCCCAGAGAACTCAGAGACT GAACCCAACCAGTACAGCCCCGATGACTCCGAGCTCTTCCAGATTGACAGTGAGAATGAGGCCCTCCTGG CAGAGCTCACCAAGACCCTGGATGACATCCCTGAAGATGACGTGGGTCTGGCTGCCTTCCCAGCCCTGGA TGGTGGAGACGCTCTATCATGCACCTCAGCTTCGCCTGCCCCCTCATCTGCACCCCCCAGCCCTGCCCCG GAGAAGCCCTCGGCCCCAGCCCCTGAGGTGGACGAGCTCTCACTGCTGCAGAAGCTCCTCCTGGCCACAT CCTACCCAACATCAAGCTCTGACACCCAGAAGGAAGGGACCGCCTGGCGCCAGGCAGGCCTCAGATCTAA AAGTCAACGGCCTTGTGTTAAGGCGGACAGCACCCAAGACAAGAAGGCTCCCATGATGCAGTCTCAGAGC CGAAGTTGTACAGAACTACATAAGCACCTCACCTCGGCACAGTGCTGCCTGCAGGATCGGGGTCTGCAGC CACCATGCCTCCAGAGTCCCCGGCTCCCTGCCAAGGAGGACAAGGAGCCGGGTGAGGACTGCCCGAGCCC CCAGCCAGCTCCAGCCTCTCCCCAGGACTCCCTAGCTCTGGGCAGGGCAGACCCCGGTGCCCCGGTTTCC CAGGAAGACATGCAGGCGATGGTGCAACTCATACGCTACATGCACACCTACTGCCTCCCCCAGAGGAAGC TGCCCCCACAGACCCCTGAGCCACTCCCCAAGGCCTGCAGCAACCCCTCCCAGCAGGTCAGATCCCGGCC CTGGTCCCGGCACCACTCCAAAGCCTCCTGGGCTGAGTTCTCCATTCTGAGGGAACTTCTGGCTCAAGAC GTGCTCTGTGATGTCAGCAAACCCTACCGTCTGGCCACGCCTGTTTATGCCTCCCTCACACCTCGGTCAA GGCCCAGGCCCCCCAAAGACAGTCAGGCCTCCCCTGGTCGCCCGTCCTCGGTGGAGGAGGTAAGGATCGC AGCTTCACCCAAGAGCACCGGGCCCAGACCAAGCCTGCGCCCACTGCGGCTGGAGGTGAAAAGGGAGGTC CGCCGGCCTGCCAGACTGCAGCAGCAGGAGGAGGAAGACGAGGAAGAAGAGGAGGAGGAAGAGGAAGAAG AAAAAGAGGAGGAGGAGGAGTGGGGCAGGAAAAGGCCAGGCCGAGGCCTGCCATGGACGAAGCTGGGGAG GAAGCTGGAGAGCTCTGTGTGCCCCGTGCGGCGTTCTCGGAGACTGAACCCTGAGCTGGGCCCCTGGCTG ACATTTGCAGATGAGCCGCTGGTCCCCTCGGAGCCCCAAGGTGCTCTGCCCTCACTGTGCCTGGCTCCCA AGGCCTACGACGTAGAGCGGGAGCTGGGCAGCCCCACGGACGAGGACAGTGGCCAAGACCAGCAGCTCCT ACGGGGACCCCAGATCCCTGCCCTGGAGAGCCCCTGTGAGAGTGGGTGTGGGGACATGGATGAGGACCCC AGCTGCCCGCAGCTCCCTCCCAGAGACTCTCCCAGGTGCCTCATGCTGGCCTTGTCACAAAGCGACCCAA CTTTTGGCAAGAAGAGCTTTGAGCAGACCTTGACAGTGGAGCTCTGTGGCACAGCAGGACTCACCCCACC CACCACACCACCGTACAAGCCCACAGAGGAGGATCCCTTCAAACCAGACATCAAGCATAGTCTAGGCAAA GAAATAGCTCTCAGCCTCCCCTCCCCTGAGGGCCTCTCACTCAAGGCCACCCCAGGGGCTGCCCACAAGC TGCCAAAGAAGCACCCAGAGCGAAGTGAGCTCCTGTCCCACCTGCGACATGCCACAGCCCAGCCAGCCTC CCAGGCTGGCCAGAAGCGTCCCTTCTCCTGTTCCTTTGGAGACCATGACTACTGCCAGGTGCTCCGACCA GAAGGCGTCCTGCAAAGGAAGGTGCTGAGGTCCTGGGAGCCGTCTGGGGTTCACCTTGAGGACTGGCCCC AGCAGGGTGCCCCTTGGGCTGAGGCACAGGCCCCTGGCAGGGAGGAAGACAGAAGCTGTGATGCTGGTGC CCCACCCAAGGACAGCACGCTGCTGAGAGACCATGAGATCCGTGCTAGCCTCACCAAACACTTTGGGCTG CTGGAGACCGCCCTGGAGGAGGAAGACCTGGCCTCCTGCAAGAGCCCTGAGTATGACACTGTCTTTGAAG ACAGCAGCAGCAGCAGCGGCGAGAGCAGCTTCCTCCCAGAGGAGGAAGAGGAAGAAGGGGAGGAGGAGGA GGAGGACGATGAAGAAGAGGACTCAGGGGTCAGCCCCACTTGCTCTGACCACTGCCCCTACCAGAGCCCA CCAAGCAAGGCCAACCGGCAGCTCTGTTCCCGCAGCCGCTCAAGCTCTGGCTCTTCACCCTGCCACTCCT GGTCACCAGCCACTCGAAGGAACTTCAGATGTGAGAGCAGAGGGCCGTGTTCAGACAGAACGCCAAGCAT CCGGCACGCCAGGAAGCGGCGGGAAAAGGCCATTGGGGAAGGCCGCGTGGTGTACATTCAAAATCTCTCC AGCGACATGAGCTCCCGAGAGCTGAAGAGGCGCTTTGAAGTGTTTGGTGAGATTGAGGAGTGCGAGGTGC TGACAAGAAATAGGAGAGGCGAGAAGTACGGCTTCATCACCTACCGGTGTTCTGAGCACGCGGCCCTCTC TTTGACAAAGGGCGCTGCCCTGAGGAAGCGCAACGAGCCCTCCTTCCAGCTGAGCTACGGAGGGCTCCGG CACTTCTGCTGGCCCAGATACACTGACTACGATTCCAATTCAGAAGAGGCCCTTCCTGCGTCAGGGAAAA GCAAGTATGAAGCCATGGATTTTGACAGCTTACTGAAAGAGGCCCAGCAGAGCCTGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224480 representing NM_133263
Red=Cloning site Green=Tags(s) MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSET EPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAP EKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQS RSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPQDSLALGRADPGAPVS QEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQD VLCDVSKPYRLATPVYASLTPRSRPRPPKDSQASPGRPSSVEEVRIAASPKSTGPRPSLRPLRLEVKREV RRPARLQQQEEEDEEEEEEEEEEEKEEEEEWGRKRPGRGLPWTKLGRKLESSVCPVRRSRRLNPELGPWL TFADEPLVPSEPQGALPSLCLAPKAYDVERELGSPTDEDSGQDQQLLRGPQIPALESPCESGCGDMDEDP SCPQLPPRDSPRCLMLALSQSDPTFGKKSFEQTLTVELCGTAGLTPPTTPPYKPTEEDPFKPDIKHSLGK EIALSLPSPEGLSLKATPGAAHKLPKKHPERSELLSHLRHATAQPASQAGQKRPFSCSFGDHDYCQVLRP EGVLQRKVLRSWEPSGVHLEDWPQQGAPWAEAQAPGREEDRSCDAGAPPKDSTLLRDHEIRASLTKHFGL LETALEEEDLASCKSPEYDTVFEDSSSSSGESSFLPEEEEEEGEEEEEDDEEEDSGVSPTCSDHCPYQSP PSKANRQLCSRSRSSSGSSPCHSWSPATRRNFRCESRGPCSDRTPSIRHARKRREKAIGEGRVVYIQNLS SDMSSRELKRRFEVFGEIEECEVLTRNRRGEKYGFITYRCSEHAALSLTKGAALRKRNEPSFQLSYGGLR HFCWPRYTDYDSNSEEALPASGKSKYEAMDFDSLLKEAQQSLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133263 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133263.2, NP_573570.2 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 133522 |
| UniProt ID | Q86YN6 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224480 | PPARGC1B (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1 |
CNY 7,128.00 |
|
| RC224480L1 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, Myc-DDK-tagged |
CNY 9,528.00 |
|
| RC224480L2 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, mGFP tagged |
CNY 12,070.00 |
|
| RC224480L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, Myc-DDK-tagged |
CNY 12,070.00 |
|
| RC224480L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, mGFP tagged |
CNY 9,528.00 |
|
| SC305937 | PPARGC1B (untagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1 |
CNY 7,128.00 |
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