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MYT1 (NM_004535) Human Tagged ORF Clone
CAT＃: RG224321
- TrueORF®
MYT1 (tGFP-tagged) - Human myelin transcription factor 1 (MYT1). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_004535" in other vectors (2)
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CNY 9,220.00
货期*
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规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C20orf36; MTF1; MYTI; NZF2; PLPB1; ZC2H2C1; ZC2HC4A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224321 ORF sequence, codon optimized.
Due to the complexity of NM_004535, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACTTGAGAACGAGGATAAACGCGCCCGCACACGGTCCAAAGCACTGAGAGGGCCACCTGAAACGA CTGCTGCAGACCTTTCATGCCCCACCCCTGGCTGCACAGGTAGCGGACATGTGCGCGGAAAGTACTCTAG GCACCGCTCACTGCAGAGTTGCCCCCTGGCCAAGAAACGCAAGCTTGAGGGCGCCGAGGCTGAACACCTT GTTTCAAAGCGGAAGTCCCACCCACTCAAACTGGCACTGGATGAAGGTTATGGAGTGGATAGCGACGGGT CTGAGGATACAGAGGTGAAAGATGCCAGCGTGAGCGACGAATCCGAGGGAACGCTGGAGGGTGCTGAAGC CGAAACCTCTGGACAAGACGAAATCCACCGGCCAGAGACGGCCGAGGGCAGATCACCGGTTAAGTCTCAC TTCGGCTCAAATCCTATAGGATCTGCAACGGCGTCTAGTAAAGGTTCCTATTCTTCTTACCAGGGCATAA TTGCTACCTCCCTGTTGAACTTGGGACAGATCGCAGAAGAGACTCTGGTAGAAGAGGACCTCGGTCAGGC AGCGAAACCCGGACCTGGTATTGTGCACCTCCTGCAGGAGGCCGCCGAGGGTGCTGCAAGTGAGGAGGGG GAAAAAGGACTGTTTATCCAGCCCGAGGACGCTGAGGAGGTCGTGGAGGTGACAACAGAACGGAGTCAGG ACCTCTGCCCTCAGAGTCTCGAGGATGCAGCGTCCGAGGAATCCTCCAAGCAGAAGGGCATCTTGTCTCA TGAGGAAGAGGATGAGGAGGAAGAGGAAGAAGAGGAAGAAGAAGAGGAGGACGAAGAGGAAGAGGAGGAG GAGGAGGAAGAAGAAGAGGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAGAGGAGGAGGAGGAGGAGGAGG AGGAGGAGGCTGCCCCTGATGTGATTTTTCAGGAAGATACCAGCCACACCTCTGCTCAAAAGGCCCCAGA ACTCCGGGGTCCCGAATCACCCTCTCCTAAGCCCGAGTACTCAGTAATCGTGGAAGTGCGCTCAGATGAC GACAAGGACGAAGATACTCATAGTCGGAAAAGCACAGTGACCGACGAGAGCGAAATGCAGGATATGATGA CCAGGGGAAACCTTGGCTTGCTGGAGCAGGCCATAGCCCTTAAGGCCGAACAGGTGAGGACTGTCTGCGA GCCGGGCTGTCCTCCTGCCGAACAGTCACAACTGGGCCTGGGGGAACCCGGCAAAGCGGCAAAGCCTCTC GACACGGTTAGGAAGAGCTATTACTCAAAGGATCCATCCCGCGCCGAGAAGCGAGAAATTAAATGTCCCA CACCCGGGTGCGATGGTACGGGGCATGTTACAGGCCTGTACCCACACCATCGATCCCTGTCTGGGTGCCC CCACAAGGATAGAATCCCCCCAGAGATTCTGGCAATGCATGAGAACGTTCTGAAGTGTCCTACCCCAGGT TGCACCGGACAGGGGCATGTAAACAGCAACCGGAACACACATCGGTCACTCTCAGGATGCCCCATTGCAG CGGCTGAGAAACTTGCGAAAAGCCACGAAAAGCAGCAACCTCAGACGGGGGACCCGTCAAAAAGCAGCTC TAATAGTGACAGGATCCTGCGGCCCATGTGCTTCGTCAAGCAGCTGGAGGTGCCCCCATACGGGAGTTAC CGGCCAAACGTGGCACCAGCCACGCCCAGGGCCAACCTTGCCAAAGAGCTGGAAAAGTTCAGCAAAGTCA CCTTTGATTATGCTTCATTCGATGCACAGGTGTTCGGTAAGCGGATGCTCGCTCCTAAGATCCAGACATC TGAGACATCACCCAAAGCTTTTCAGTGTTTCGATTATTCCCAGGATGCAGAAGCAGCTCATATGGCTGCA ACAGCTATACTGAATCTGAGTACGAGGTGCTGGGAGATGCCAGAAAACTTGTCAACAAAGCCTCAGGACT TGCCCAGTAAATCCGTAGACATCGAAGTGGATGAGAATGGTACGCTGGATTTGTCAATGCACAAGCATAG AAAAAGAGAGAATGCGTTCCCAAGCTCAAGCAGTTGCTCTAGCTCACCAGGGGTTAAGTCCCCAGACGCA AGTCAGCGCCATAGTTCCACCTCCGCCCCTAGCTCATCAATGACAAGTCCTCAGTCTAGCCAGGCGTCCA GACAGGACGAGTGGGACAGGCCACTGGACTATACGAAGCCCAGCCGGCTCCGGGAAGAAGAACCAGAGGA GTCTGAGCCCGCGGCCCATAGTTTTGCCTCATCCGAGGCAGATGACCAGGAAGTGTCCGAAGAAAACTTT GAGGAGCGAAAATATCCGGGTGAGGTTACCCTCACCAATTTCAAGCTCAAGTTCCTGTCTAAGGACATCA AAAAAGAGCTGCTCACATGCCCCACGCCTGGCTGCGATGGAAGCGGGCATATAACCGGGAACTATGCAAG CCATAGATCACTCAGTGGCTGTCCCCTGGCCGACAAAAGTCTCAGAAATCTGATGGCCGCCCACAGCGCG GATCTCAAGTGCCCAACCCCAGGATGCGACGGAAGTGGCCACATCACTGGGAATTACGCCTCCCACCGGA GCCTCAGCGGGTGCCCAAGGGCTAAAAAAAGCGGAGTAAAGGTGGCTCCCACTAAGGATGACAAGGAGGA TCCCGAGCTGATGAAATGCCCTGTTCCAGGCTGTGTTGGGCTTGGCCATATCTCCGGGAAGTACGCTAGT CACCGCTCTGCCAGCGGATGCCCCCTGGCTGCTAGGCGCCAGAAAGAGGGGTCCCTGAACGGAAGTTCCT TCTCCTGGAAGAGCCTGAAAAATGAGGGCCCTACGTGTCCTACGCCTGGATGTGATGGATCAGGCCACGC AAATGGCAGCTTTTTGACACACCGCTCTCTTTCCGGGTGCCCCAGGGCAACCTTTGCCGGGAAAAAGGGA AAACTTAGCGGGGATGAAGTTCTCTCTCCTAAATTCAAGACATCCGACGTGCTGGAGAATGATGAAGAAA TCAAGCAGCTGAATCAGGAGATTCGAGATTTGAACGAAAGCAACTCAGAAATGGAGGCTGCCATGGTGCA GCTCCAGAGCCAGATTAGCAGCATGGAGAAGAACCTCAAGAATATAGAAGAAGAAAACAAGCTTATAGAG GAGCAAAACGAGGCCCTGTTTCTGGAGTTGTCAGGCCTCAGCCAGGCACTGATTCAGTCCCTGGCTAATA TTAGGCTTCCGCACATGGAACCAATTTGTGAGCAAAACTTTGATGCATACGTCTCCACGCTTACAGATAT GTATTCTAACCAGGACCCCGAGAACAAAGACCTCCTGGAATCAATCAAACAGGCCGTGAGGGGAATTCAG GTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224321 representing NM_004535
Red=Cloning site Green=Tags(s) MSLENEDKRARTRSKALRGPPETTAADLSCPTPGCTGSGHVRGKYSRHRSLQSCPLAKKRKLEGAEAEHL VSKRKSHPLKLALDEGYGVDSDGSEDTEVKDASVSDESEGTLEGAEAETSGQDEIHRPETAEGRSPVKSH FGSNPIGSATASSKGSYSSYQGIIATSLLNLGQIAEETLVEEDLGQAAKPGPGIVHLLQEAAEGAASEEG EKGLFIQPEDAEEVVEVTTERSQDLCPQSLEDAASEESSKQKGILSHEEEDEEEEEEEEEEEEDEEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEAAPDVIFQEDTSHTSAQKAPELRGPESPSPKPEYSVIVEVRSDD DKDEDTHSRKSTVTDESEMQDMMTRGNLGLLEQAIALKAEQVRTVCEPGCPPAEQSQLGLGEPGKAAKPL DTVRKSYYSKDPSRAEKREIKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRIPPEILAMHENVLKCPTPG CTGQGHVNSNRNTHRSLSGCPIAAAEKLAKSHEKQQPQTGDPSKSSSNSDRILRPMCFVKQLEVPPYGSY RPNVAPATPRANLAKELEKFSKVTFDYASFDAQVFGKRMLAPKIQTSETSPKAFQCFDYSQDAEAAHMAA TAILNLSTRCWEMPENLSTKPQDLPSKSVDIEVDENGTLDLSMHKHRKRENAFPSSSSCSSSPGVKSPDA SQRHSSTSAPSSSMTSPQSSQASRQDEWDRPLDYTKPSRLREEEPEESEPAAHSFASSEADDQEVSEENF EERKYPGEVTLTNFKLKFLSKDIKKELLTCPTPGCDGSGHITGNYASHRSLSGCPLADKSLRNLMAAHSA DLKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGVKVAPTKDDKEDPELMKCPVPGCVGLGHISGKYAS HRSASGCPLAARRQKEGSLNGSSFSWKSLKNEGPTCPTPGCDGSGHANGSFLTHRSLSGCPRATFAGKKG KLSGDEVLSPKFKTSDVLENDEEIKQLNQEIRDLNESNSEMEAAMVQLQSQISSMEKNLKNIEEENKLIE EQNEALFLELSGLSQALIQSLANIRLPHMEPICEQNFDAYVSTLTDMYSNQDPENKDLLESIKQAVRGIQ V TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004535 |
| ORF Size | 3363 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004535.2, NP_004526.1 |
| RefSeq Size | 5557 bp |
| RefSeq ORF | 3366 bp |
| Locus ID | 4661 |
| UniProt ID | Q01538 |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene is a member of a family of neural specific, zinc finger-containing DNA-binding proteins. The protein binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. [provided by RefSeq, Jul 2008] |
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