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DIS (CCAR1) (NM_018237) Human Tagged ORF Clone
CAT＃: RG224293
- TrueORF®
CCAR1 (tGFP-tagged) - Human cell division cycle and apoptosis regulator 1 (CCAR1)
ORF Plasmid: DDK
"NM_018237" in other vectors (6)
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CNY 9,608.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224293 representing NM_018237
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAATTTGGAGGACAGAAGAATCCGCCATGGGCTACTCAGTTTACAGCCACTGCAGTATCACAGC CAGCTGCACTGGGTGTTCAACAGCCATCACTCCTTGGAGCATCTCCTACCATTTATACACAGCAAACTGC ATTGGCAGCAGCAGGCCTTACCACACAAACTCCAGCAAACTATCAGTTAACACAAACTGCTGCATTGCAG CAACAAGCCGCAGCTGCAGCAGCTGCATTACAACAGCAATATTCACAACCTCAGCAGGCCCTGTATAGTG TGCAACAACAGTTACAGCAACCCCAGCAAACCCTCTTAACACAGCCAGCTGTTGCACTGCCTACAAGCCT TAGCCTGTCTACTCCTCAGCCAACAGCACAAATAACTGTATCATATCCAACACCAAGGTCCAGTCAACAG CAAACCCAGCCTCAGAAGCAGCGTGTTTTCACAGGGGTGGTTACAAAACTACATGATACGTTTGGATTTG TGGATGAAGATGTATTCTTTCAGCTTAGTGCTGTCAAAGGGAAAACCCCCCAAGTAGGTGACAGAGTATT GGTTGAAGCTACTTATAATCCTAATATGCCTTTTAAATGGAATGCACAGAGAATTCAAACACTACCAAAT CAGAATCAGTCGCAAACCCAGCCATTACTGAAGACTCCTCCTGCTGTACTTCAGCCAATTGCACCACAGA CAACATTTGGTGTTCAGACTCAGCCCCAGCCCCAGTCACTGCTGCAGGCACAGATTTCAGCAGCTTCTAT TACACCACTATTGCAGACTCAACCACAGCCCTTATTACAGCAGCCTCAGCAAAAAGCTGGTTTATTGCAG CCTCCTGTTCGTATAGTTTCACAGCCACAACCGGCACGACGATTAGATCCCCCATCCCGATTTTCAGGAA GAAATGACAGAGGGGATCAAGTGCCTAACAGAAAAGATGATCGAAGTCGTGAGAGAGAGAGAGAAAGACG TAGATCGAGAGAAAGATCACCTCAGAGGAAACGTTCCCGGGAAAGATCTCCACGAAGAGAGCGAGAGCGA TCACCTCGGAGAGTTCGACGTGTTGTTCCACGTTACACAGTTCAGTTTTCAAAGTTTTCTTTAGATTGTC CCAGTTGTGACATGATGGAACTAAGGCGCCGTTATCAAAATTTGTATATACCTAGTGACTTTTTTGATGC TCAATTTACATGGGTGGATGCTTTCCCTTTGTCAAGACCATTTCAGCTGGGAAATTACTGCAATTTTTAT GTAATGCACAGAGAAGTAGAGTCCTTAGAAAAAAATATGGCCATTCTTGATCCACCAGATGCTGACCACT TATACAGTGCAAAGGTAATGCTGATGGCTAGCCCTAGTATGGAAGATTTATATCATAAGTCATGTGCTCT TGCTGAGGACCCACAAGAACTTCGAGATGGATTCCAACATCCTGCTAGACTTGTTAAGTTTTTAGTGGGC ATGAAAGGCAAGGATGAAGCTATGGCCATTGGAGGCCACTGGTCTCCTTCGTTGGATGGACCAGACCCAG AAAAAGATCCCTCTGTGTTGATTAAGACTGCTATTCGTTGTTGTAAGGCTCTGACAGGCATTGATCTAAG TGTGTGCACACAATGGTACCGTTTTGCAGAGATTCGCTACCATCGCCCTGAGGAGACCCACAAGGGGCGT ACAGTTCCAGCTCATGTGGAGACAGTGGTTTTATTTTTCCCGGATGTTTGGCATTGCCTTCCCACCCGCT CAGAGTGGGAAACCCTCTCCCGAGGATACAAGCAGCAGCTGGTCGAGAAGCTTCAGGGTGAACGCAAGGA GGCTGATGGAGAACAGGATGAAGAAGAGAAGGATGATGGTGAAGCTAAAGAAATTTCTACACCTACCCAT TGGTCTAAACTTGATCCAAAGACAATGAAGGTAAATGACCTCCGAAAAGAATTAGAAAGTCGAGCTCTTA GTTCCAAAGGATTAAAATCCCAGTTAATAGCCCGATTGACAAAACAGCTTAAAGTAGAGGAACAAAAAGA AGAACAGAAGGAGTTAGAGAAATCTGAAAAAGAAGAGGATGAGGATGATGATAGGAAATCTGAAGACGAT AAAGAGGAAGAAGAAAGGAAACGTCAAGAGGAAATAGAACGCCAGCGTCGAGAAAGAAGATATATTTTGC CTGATGAACCGGCCATCATTGTACATCCAAATTGGGCTGCAAAAAGTGGCAAGTTTGATTGTAGCATCAT GTCTTTGAGTGTCCTATTGGACTACAGATTAGAGGATAATAAAGAACATTCATTTGAGGTTTCATTGTTT GCGGAACTTTTCAACGAAATGCTTCAAAGAGATTTTGGTGTCCGTATATACAAATCATTACTGTCTCTTC CTGAGAAAGAGGACAAAAAAGAAAAGGATAAAAAAAGCAAAAAAGATGAGAGAAAAGATAAAAAAGAAGA AAGAGATGATGAAACTGATGAACCAAAACCCAAACGGAGAAAATCAGGCGATGATAAAGATAAAAAAGAA GATAGAGATGAAAGGAAGAAAGAAGATAAAAGAAAAGATGATTCTAAAGATGATGATGAAACTGAAGAAG ATAACAATCAAGATGAATATGACCCTATGGAAGCAGAAGAAGCTGAGGATGAAGAAGATGATAGGGATGA GGAAGAAATGACCAAACGAGATGACAAAAGAGATATCAACAGATACTGCAAGGAGAGGCCCTCTAAAGAT AAGGAAAAAGAAAAGACTCAAATGATCACAATTAACAGAGATCTGTTAATGGCTTTTGTTTATTTTGATC AAAGTCATTGTGGTTACCTTCTTGAAAAGGATTTGGAAGAAATACTTTATACTCTTGGACTACATCTTTC TCGGGCTCAGGTAAAGAAGCTTCTTAATAAAGTAGTGCTCCGTGAATCTTGCTTTTACCGGAAATTAACA GACACCTCAAAAGATGAAGAGAACCATGAAGAGTCTGAGTCATTGCAGGAAGATATGCTAGGAAACAGAT TATTACTTCCAACACCAACAGTAAAGCAGGAATCAAAGGATGTGGAAGAAAATGTTGGCCTCATTGTGTA CAATGGTGCAATGGTAGATGTAGGAAGCCTCTTGCAAAAATTGGAAAAGAGCGAAAAAGTAAGAGCTGAG GTAGAACAGAAGCTGCAGTTACTAGAAGAAAAAACAGATGAAGATGAAAAAACCATATTAAATTTGGAGA ATTCCAACAAAAGCCTCTCTGGTGAACTCAGAGAAGTTAAAAAGGACCTTAGTCAGTTACAAGAAAACTT AAAGATTTCGGAAAACATGAATTTACAATTTGAAAACCAAATGAATAAGACAATCAGAAACTTATCTACG GTAATGGATGAAATCCACACTGTTCTCAAGAAGGATAATGTAAAGAATGAAGACAAAGATCAAAAATCCA AGGAGAATGGTGCCAGTGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224293 representing NM_018237
Red=Cloning site Green=Tags(s) MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQ QQAAAAAAALQQQYSQPQQALYSVQQQLQQPQQTLLTQPAVALPTSLSLSTPQPTAQITVSYPTPRSSQQ QTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPN QNQSQTQPLLKTPPAVLQPIAPQTTFGVQTQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQ PPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERERERRRSRERSPQRKRSRERSPRRERER SPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFY VMHREVESLEKNMAILDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQELRDGFQHPARLVKFLVG MKGKDEAMAIGGHWSPSLDGPDPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGR TVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKEADGEQDEEEKDDGEAKEISTPTH WSKLDPKTMKVNDLRKELESRALSSKGLKSQLIARLTKQLKVEEQKEEQKELEKSEKEEDEDDDRKSEDD KEEEERKRQEEIERQRRERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLF AELFNEMLQRDFGVRIYKSLLSLPEKEDKKEKDKKSKKDERKDKKEERDDETDEPKPKRRKSGDDKDKKE DRDERKKEDKRKDDSKDDDETEEDNNQDEYDPMEAEEAEDEEDDRDEEEMTKRDDKRDINRYCKERPSKD KEKEKTQMITINRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLT DTSKDEENHEESESLQEDMLGNRLLLPTPTVKQESKDVEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAE VEQKLQLLEEKTDEDEKTILNLENSNKSLSGELREVKKDLSQLQENLKISENMNLQFENQMNKTIRNLST VMDEIHTVLKKDNVKNEDKDQKSKENGASV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018237 |
| ORF Size | 3450 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018237.4 |
| RefSeq Size | 3858 bp |
| RefSeq ORF | 3453 bp |
| Locus ID | 55749 |
| UniProt ID | Q8IX12 |
| Gene Summary | Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224293 | CCAR1 (Myc-DDK-tagged)-Human cell division cycle and apoptosis regulator 1 (CCAR1) |
CNY 8,008.00 |
|
| RC224293L1 | Lenti ORF clone of Human cell division cycle and apoptosis regulator 1 (CCAR1), Myc-DDK-tagged |
CNY 10,408.00 |
|
| RC224293L2 | Lenti ORF clone of Human cell division cycle and apoptosis regulator 1 (CCAR1), mGFP tagged |
CNY 10,260.00 |
|
| RC224293L3 | Lenti ORF clone of Human cell division cycle and apoptosis regulator 1 (CCAR1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC224293L4 | Lenti ORF clone of Human cell division cycle and apoptosis regulator 1 (CCAR1), mGFP tagged |
CNY 10,260.00 |
|
| SC311324 | CCAR1 (untagged)-Human cell division cycle and apoptosis regulator 1 (CCAR1) |
CNY 10,450.00 |
