SLC5A7 (NM_021815) Human Tagged ORF Clone
CAT#: RG224138
- TrueORF®
SLC5A7 (tGFP-tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7)
ORF Plasmid: DDK
"NM_021815" in other vectors (6)
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CNY 8,080.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CHT; CHT1; CMS20; HMN7A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG224138 representing NM_021815
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTGGTTGGAATAT GGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAAGCCATCATAGTTGGTGGCCG AGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCTACCTGGGTCGGAGGAGGGTATATCAATGGC ACAGCTGAAGCAGTTTATGTACCAGGTTATGGCCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTA GTCTGATTTTAGGTGGCCTGTTCTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCC GTTTCAGCAAATCTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTC TGGGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGATATGCACATTT CTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGGGAGGGCTCTATTCTGTGGCCTACAC TGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGGATCAGCGTCCCCTTTGCATTGTCACATCCT GCAGTCGCAGACATCGGGTTCACTGCTGTGCATGCCAAATACCAAAAGCCGTGGCTGGGAACTGTTGACT CATCTGAAGTCTACTCTTGGCTTGATAGTTTTCTGTTGTTGATGCTGGGTGGAATCCCATGGCAAGCATA CTTTCAGAGGGTTCTCTCTTCTTCCTCAGCCACCTATGCTCAAGTGCTGTCCTTCCTGGCAGCTTTCGGG TGCCTGGTGATGGCCATCCCAGCCATACTCATTGGGGCCATTGGAGCATCAACAGACTGGAACCAGACTG CATATGGGCTTCCAGATCCCAAGACTACAGAAGAGGCAGACATGATTTTACCAATTGTTCTGCAGTATCT CTGCCCTGTGTATATTTCTTTCTTTGGTCTTGGTGCAGTTTCTGCTGCTGTTATGTCATCAGCAGATTCT TCCATCTTGTCAGCAAGTTCCATGTTTGCACGGAACATCTACCAGCTTTCCTTCAGACAAAATGCTTCGG ACAAAGAAATCGTTTGGGTTATGCGAATCACAGTGTTTGTGTTTGGAGCATCTGCAACAGCCATGGCCTT GCTGACGAAAACTGTGTATGGGCTCTGGTACCTCAGTTCTGACCTTGTTTACATCGTTATCTTCCCCCAG CTGCTTTGTGTACTCTTTGTTAAGGGAACCAACACCTATGGGGCCGTGGCAGGTTATGTTTCTGGCCTCT TCCTGAGAATAACTGGAGGGGAGCCATATCTGTATCTTCAGCCCTTGATCTTCTACCCTGGCTATTACCC TGATGATAATGGTATATATAATCAGAAATTTCCATTTAAAACACTTGCCATGGTTACATCATTCTTAACC AACATTTGCATCTCCTATCTAGCCAAGTATCTATTTGAAAGTGGAACCTTGCCACCTAAATTAGATGTAT TTGATGCTGTTGTTGCAAGACACAGTGAAGAAAACATGGATAAGACAATTCTTGTCAAAAATGAAAATAT TAAATTAGATGAACTTGCACTTGTGAAGCCACGACAGAGCATGACCCTCAGCTCAACTTTCACCAATAAA GAGGCCTTCCTTGATGTTGATTCCAGTCCAGAAGGGTCTGGGACTGAAGATAATTTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224138 representing NM_021815
Red=Cloning site Green=Tags(s) MAFHVEGLIAIIVFYLLILLVGIWAAWRTKNSGSAEERSEAIIVGGRDIGLLVGGFTMTATWVGGGYING TAEAVYVPGYGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGEMF WAAAIFSALGATISVIIDVDMHISVIISALIATLYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFALSHP AVADIGFTAVHAKYQKPWLGTVDSSEVYSWLDSFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFG CLVMAIPAILIGAIGASTDWNQTAYGLPDPKTTEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADS SILSASSMFARNIYQLSFRQNASDKEIVWVMRITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQ LLCVLFVKGTNTYGAVAGYVSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFKTLAMVTSFLT NICISYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLDELALVKPRQSMTLSSTFTNK EAFLDVDSSPEGSGTEDNLQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021815 |
ORF Size | 1740 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021815.5 |
RefSeq Size | 5158 bp |
RefSeq ORF | 1743 bp |
Locus ID | 60482 |
UniProt ID | Q9GZV3 |
Domains | SSF |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224138 | SLC5A7 (Myc-DDK-tagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
CNY 6,480.00 |
|
RC224138L1 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), Myc-DDK-tagged |
CNY 8,880.00 |
|
RC224138L2 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
CNY 8,880.00 |
|
RC224138L3 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC224138L4 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
CNY 6,460.00 |
|
SC112868 | SLC5A7 (untagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
CNY 6,488.00 |