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Tensin 1 (TNS1) (NM_022648) Human Tagged ORF Clone
CAT＃: RG224050
- TrueORF®
TNS1 (tGFP-tagged) - Human tensin 1 (TNS1)
ORF Plasmid: DDK
"NM_022648" in other vectors (2)
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CNY 19,096.00
货期*
现货
规格
| Cited in 1 publication. |
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MST091; MST122; MST127; MSTP091; MSTP122; MSTP127; MXRA6; PPP1R155; TNS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224050 representing NM_022648
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGAGCCGGACCATGGAGGACAGCTGTGAGCTGGACCTGGTGTACGTCACAGAGAGGATCATCG CTGTCTCCTTCCCCAGCACAGCCAATGAGGAGAACTTCCGGAGCAACCTCCGTGAGGTGGCGCAGATGCT CAAGTCCAAACATGGAGGCAACTACCTGCTGTTCAACCTCTCTGAGCGGAGACCTGACATCACGAAGCTC CATGCCAAGGTACTGGAATTTGGCTGGCCCGACCTCCACACCCCAGCCCTGGAGAAGATCTGCAGCATCT GTAAGGCCATGGACACATGGCTCAATGCAGACCCTCACAATGTCGTTGTTCTACACAACAAGGGAAACCG AGGCAGGATAGGAGTTGTCATCGCGGCTTACATGCACTACAGCAACATTTCTGCCAGTGCGGACCAGGCT CTGGACCGGTTTGCAATGAAGCGGTTCTATGAGGATAAGATTGTGCCCATTGGCCAGCCATCCCAAAGAA GGTACGTGCATTACTTCAGTGGCCTGCTCTCCGGCTCCATCAAAATGAACAACAAGCCCTTGTTTCTGCA CCACGTGATCATGCACGGCATCCCCAACTTTGAGTCTAAAGGAGGATGTCGGCCATTTCTCCGCATCTAC CAGGCCATGCAACCTGTGTACACATCTGGCATCTACAACATCCCAGGAGACAGCCAGACTAGCGTCTGCA TCACCATCGAGCCAGGACTGCTCTTGAAGGGAGACATCTTGCTGAAGTGCTACCACAAGAAGTTCCGAAG CCCAGCCCGAGACGTCATCTTCCGTGTGCAGTTCCACACCTGTGCCATCCATGACCTGGGGGTTGTCTTT GGGAAGGAGGACCTTGATGATGCTTTCAAAGATGATCGATTTCCAGAGTATGGCAAAGTGGAGTTTGTAT TTTCTTATGGGCCAGAGAAAATTCAAGGCATGGAGCACCTGGAGAACGGGCCGAGCGTGTCTGTGGACTA TAACACCTCCGACCCCCTCATCCGCTGGGACTCCTACGACAACTTCAGTGGGCATCGAGATGACGGCATG GAGGAGGTGGTGGGACACACGCAGGGGCCACTAGATGGGAGCCTGTATGCTAAGGTGAAGAAGAAAGACT CCCTGCACGGCAGCACCGGGGCTGTTAATGCCACACGTCCTACACTGTCGGCCACCCCCAACCACGTGGA ACACACGCTTTCTGTGAGCAGCGACTCGGGCAACTCCACAGCCTCCACCAAGACCGACAAGACCGACGAG CCTGTCCCCGGGGCCTCCAGTGCCACTGCTGCCTTGAGTCCCCAGGAGAAGCGGGAGCTGGACCGCCTGC TGAGTGGCTTTGGCTTAGAGCGAGAGAAGCAAGGCGCCATGTACCACACCCAGCACCTCAGGTCCCGCCC AGCAGGGGGCTCGGCTGTGCCCTCCTCTGGACGCCACGTTGTCCCAGCCCAGGTTCATGTCAATGGTGGG GCGTTAGCATCTGAGCGGGAGACAGACATCCTGGACGATGAATTGCCAAACCAGGATGGTCACAGTGCGG GCAGCATGGGCACACTCTCTTCTCTGGACGGGGTCACCAACACCAGTGAGGGGGGCTACCCAGAGGCCCT GTCCCCACTGACCAACGGTCTGGACAAGTCCTACCCCATGGAGCCTATGGTCAATGGAGGAGGCTACCCC TACGAGTCTGCCAGCCGGGCGGGGCCTGCCCATGCTGGCCACACGGCCCCCATGCGGCCCTCCTACTCTG CACAGGAGGGTTTAGCTGGCTACCAGAGGGAGGGGCCCCACCCAGCCTGGCCACAGCCAGTGACCACCTC CCACTATGCCCATGACCCCAGCGGTATGTTCCGCTCTCAATCCTTTTCGGAAGCTGAACCCCAGCTGCCC CCAGCTCCGGTCCGAGGGGGAAGCAGCCGGGAGGCTGTGCAAAGGGGACTGAATTCGTGGCAGCAGCAGC AGCAGCAGCAGCAGCAGCCTCGCCCACCTCCACGCCAGCAGGAAAGAGCCCACTTGGAGAGTCTTGTAGC CAGCAGGCCCAGCCCTCAGCCATTGGCAGAGACCCCCATCCCCAGTCTCCCTGAGTTCCCGCGAGCAGCC TCCCAGCAGGAGATTGAACAGTCCATCGAAACACTCAATATGCTGATGCTGGACCTGGAGCCAGCCTCCG CTGCTGCCCCACTACACAAGTCCCAGAGTGTCCCCGGGGCCTGGCCAGGGGCTTCTCCACTCTCCTCCCA GCCCCTCTCTGGATCCTCCCGTCAGTCCCATCCACTGACCCAGTCCAGATCTGGCTATATCCCCAGTGGG CATTCGTTGGGAACCCCTGAGCCAGCCCCACGGGCCTCTCTGGAGTCTGTCCCTCCTGGCAGGTCTTACT CACCTTATGACTATCAGCCATGTTTGGCTGGGCCTAACCAGGATTTCCATTCAAAGAGCCCAGCCTCTTC CTCCTTGCCTGCCTTCCTTCCGACCACCCACAGCCCTCCAGGGCCTCAGCAACCCCCAGCCTCTCTCCCT GGCCTCACTGCTCAGCCTCTGCTCTCACCAAAGGAAGCGACTTCAGACCCCTCCCGGACTCCAGAGGAGG AGCCATTGAATTTAGAAGGGCTGGTGGCCCACAGGGTAGCAGGGGTACAGGCTCGGGAGAAGCAGCCTGC AGAGCCCCCAGCCCCTCTGCGGAGGCGGGCGGCCAGTGATGGACAGTATGAGAACCAGTCTCCAGAAGCC ACATCCCCTCGTAGCCCTGGGGTTCGCTCCCCTGTCCAGTGTGTCTCCCCGGAGCTGGCTCTTACCATCG CTCTCAATCCTGGAGGGCGGCCCAAAGAGCCCCATTTGCACAGCTACAAGGAGGCCTTCGAGGAGATGGA GGGAACCTCCCCGAGCAGCCCACCACCCAGTGGGGTGCGGTCCCCCCCGGGTCTGGCCAAGACACCCCTG TCTGCTCTGGGCCTGAAACCTCACAACCCAGCGGACATCCTGTTGCACCCCACAGGAGTTACCAGAAGAC GGATCCAGCCAGAGGAAGATGAGGGGAAGGTGGTGGTCAGGCTGTCAGAAGAGCCCCGGAGCTATGTGGA GTCTGTGGCACGGACAGCGGTGGCTGGACCCCGAGCTCAGGACTCTGAGCCCAAGAGCTTTAGTGCTCCA GCCACCCAGGCCTATGGCCATGAGATACCCCTGAGGAACGGGACCCTGGGTGGCTCCTTTGTCTCCCCCA GCCCCCTCTCCACCAGCAGCCCCATCCTCAGTGCTGACAGCACTTCAGTGGGGAGTTTCCCGTCGGGAGA GAGCAGTGACCAGGGTCCCCGGACGCCCACCCAGCCTCTGTTGGAGTCTGGCTTCCGCTCAGGCAGCCTG GGACAGCCCAGCCCGTCTGCCCAGAGAAACTACCAGAGCTCTTCTCCTCTCCCGACTGTGGGCAGTAGCT ACAGCAGCCCCGACTACTCACTTCAGCATTTCAGCTCCTCTCCGGAAAGCCAGGCTCGAGCTCAGTTCAG TGTGGCTGGCGTCCACACGGTGCCTGGGAGCCCTCAGGCGCGCCACAGAACAGTGGGCACCAACACTCCC CCTAGTCCTGGCTTCGGCTGGCGGGCCATCAATCCCAGCATGGCTGCCCCCAGCAGTCCCAGTTTGAGCC ATCACCAGATGATGGGTCCACCAGGCACTGGCTTCCATGGTAGCACTGTCTCCAGCCCCCAGAGCAGTGC AGCGACCACCCCGGGGAGCCCCAGCCTGTGTCGGCACCCAGCAGGGGTCTACCAGGTTTCTGGCCTCCAC AACAAAGTGGCCACCACCCCGGGGAGTCCCAGCCTGGGCCGGCACCCTGGGGCTCACCAAGGCAACCTGG CCTCCGGTCTTCATAGCAATGCAATAGCCAGCCCTGGAAGCCCCAGCCTGGGCCGTCACCTCGGAGGGTC TGGATCTGTGGTTCCCGGCAGCCCCTGCTTGGACCGGCATGTGGCCTATGGTGGCTATTCTACCCCGGAG GATCGGAGACCCACACTGTCCCGGCAGAGCAGTGCCTCTGGCTACCAGGCTCCTTCCACGCCCTCCTTCC CTGTCTCCCCTGCCTACTACCCTGGCCTGAGCAGCCCTGCCACCTCCCCGTCACCAGACTCCGCAGCCTT CCGGCAAGGGAGCCCAACACCAGCCTTGCCAGAGAAGCGAAGGATGTCAGTGGGAGACCGGGCAGGCAGC CTCCCCAACTATGCCACCATCAATGGGAAGGTGTCTTCGCCTGTCGCCAGCGGCATGTCCAGTCCCAGTG GGGGCAGCACCGTCTCCTTCTCCCACACTCTGCCCGACTTCTCCAAGTACTCCATGCCAGACAACAGCCC GGAGACGCGGGCTAAAGTGAAGTTTGTCCAGGACACTTCTAAGTATTGGTACAAGCCTGAGATCTCCAGG GAGCAGGCCATCGCGCTCCTCAAGGACCAGGAGCCGGGGGCCTTCATCATCCGCGACAGTCACTCCTTCC GAGGCGCGTACGGGCTGGCCATGAAGGTGTCTTCGCCACCTCCAACCATCATGCAGCAGAATAAAAAAGG AGACATGACCCATGAGCTGGTCAGGCATTTTCTGATAGAGACTGGCCCCAGAGGAGTCAAGCTCAAGGGC TGCCCCAATGAGCCAAACTTCGGATCGCTGTCTGCCCTGGTCTACCAGCACTCCATCATCCCATTGGCCC TGCCTTGCAAGCTGGTCATTCCAAACCGAGACCCCACAGATGAATCGAAAGATAGCTCCGGCCCTGCCAA CTCAACTGCAGACCTGCTGAAACAAGGGGCAGCCTGCAATGTGCTCTTCGTCAACTCTGTGGACATGGAG TCACTCACTGGGCCACAGGCCATCTCTAAAGCCACATCTGAGACGTTGGCTGCAGACCCCACGCCAGCTG CCACCATCGTTCACTTCAAAGTCTCTGCCCAGGGAATCACTCTGACTGACAACCAGAGAAAGCTCTTTTT CAGACGCCACTACCCTCTCAACACTGTCACCTTCTGTGACCTGGATCCACAGGAAAGAAAGTGGATGAAA ACAGAGGGTGGTGCCCCTGCTAAGCTCTTCGGCTTCGTGGCCCGGAAGCAGGGCAGCACCACGGACAACG CCTGCCACCTCTTTGCTGAGCTTGACCCCAACCAGCCGGCCTCTGCCATCGTCAACTTCGTCTCCAAGGT CATGCTGAATGCCGGCCAAAAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224050 representing NM_022648
Red=Cloning site Green=Tags(s) MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKL HAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQA LDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIY QAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVF GKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGM EEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDE PVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGG ALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYP YESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLP PAPXXRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRA ASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPS GHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASL PGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPE ATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTP LSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSA PATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGS LGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNT PPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGL HNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTP EDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAG SLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEIS REQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLK GCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDM ESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWM KTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022648 |
| ORF Size | 5205 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022648.6 |
| RefSeq Size | 10276 bp |
| RefSeq ORF | 5208 bp |
| Locus ID | 7145 |
| UniProt ID | Q9HBL0 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MAP4K4 regulates integrin-FERM binding to control endothelial cell motility
,Vitorino, P;Yeung, S;Crow, A;Bakke, J;Smyczek, T;West, K;McNamara, E;Eastham-Anderson, J;Gould, S;Harris, SF;Ndubaku, C;Ye, W;,
Nature
,PubMed ID 25799996
[TNS1]
|
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