NBL1 (NM_182744) Human Tagged ORF Clone
CAT#: RG223990
- TrueORF®
NBL1 (tGFP-tagged) - Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 1
ORF Plasmid: DDK
"NM_182744" in other vectors (4)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D1S1733E; DAN; DAND1; NB; NO3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223990 representing NM_182744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGCAACCTCATGAGTCAGACAAGCAGGGCTGTGTCTATCTGGAAGTTTCCAGCCAAGCTAGGTA AAACACATGGACACAGGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTCCTGGTGGGGGCTGTCCTCCC TGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTGGCACTGTTCCCAGATAAGAGTGCCTGGTGC GAAGCCAAGAACATCACCCAGATCGTGGGCCACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGT GCCTAGGACAGTGCTTCAGCTACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTG TGACTCCTGCATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGTC ACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAGCCCGGCACCCACCCTCACCC CCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCCGAGGACCCCCCTGGGGCCCCCCACACAGAG GAAGAGGGGGCTGAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223990 representing NM_182744
Red=Cloning site Green=Tags(s) MPGNLMSQTSRAVSIWKFPAKLGKTHGHRALEATGMMLRVLVGAVLPAMLLAAPPPINKLALFPDKSAWC EAKNITQIVGHSGCEAKSIQNRACLGQCFSYSVPNTFPQSTESLVHCDSCMPAQSMWEIVTLECPGHEEV PRVDKLVEKILHCSCQACGKEPSHEGLSVYVQGEDGPGSQPGTHPHPHPHPHPGGQTPEPEDPPGAPHTE EEGAED TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_182744 |
ORF Size | 648 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_182744.3, NP_877421.2 |
RefSeq Size | 2079 bp |
RefSeq ORF | 651 bp |
Locus ID | 4681 |
UniProt ID | P41271 |
Protein Families | Secreted Protein |
Gene Summary | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223990 | NBL1 (Myc-DDK-tagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 1 |
CNY 3,990.00 |
|
RC223990L3 | Lenti-ORF clone of NBL1 (Myc-DDK-tagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 1 |
CNY 5,890.00 |
|
RC223990L4 | Lenti-ORF clone of NBL1 (mGFP-tagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 1 |
CNY 5,890.00 |
|
SC317320 | NBL1 (untagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 1 |
CNY 3,990.00 |