Retinol dehydrogenase 16 (RDH16) (NM_003708) Human Tagged ORF Clone
CAT#: RG223973
- TrueORF®
RDH16 (tGFP-tagged) - Human retinol dehydrogenase 16 (all-trans) (RDH16)
ORF Plasmid: DDK
"NM_003708" in other vectors (6)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | hRDH-E; RODH-4; SDR9C8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223973 representing NM_003708
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTCTACCTGGCGGTTTTCGTGGGCCTGTACTACCTTCTGCACTGGTACCGGGAGAGGCAGGTGC TGAGCCACCTGAGAGATAAGTATGTGTTCATCACGGGCTGTGACTCTGGCTTCGGGAAACTGCTGGCCAG ACAGCTGGATGCACGAGGCTTGCGGGTGCTGGCTGCATGTCTGACGGAGAAAGGAGCCGAGCAGCTGAGG GGCCAGACTTCAGACAGGCTGGAGACGGTGACCCTGGATGTTACCAAGACAGAGAGCGTTGCTGCAGCCG CCCAGTGGGTGAAGGAGTGCGTGAGAGACAAAGGACTCTGGGGCCTGGTGAATAATGCTGGCATCTCCTT GCCCACGGCTCCCAATGAGTTGCTCACCAAGCAGGACTTGCTGACCATACTGGACGTGAACTTGTTGGGG GTGATTGATGTGACTCTGAACCTGCTGCCCTTAGTGAGGAGGGCCAGGGGCCGTGTGGTCAACGTCTTCA GTGTCATGGGCCGGGTGTCACTTTTTGGTGGAGGCTACTGCATCTCCAAGTATGGCGTGGAAGCCTTCTC TGACTCCCTCAGGAGGGAACTCTCCTACTTTGGGGTGAAGGTGGCTATGATTGAACCTGGCTATTTCAAG ACTGCTGTGACCAGTAAGGAGAGATTCTTAAAGAGCTTCCTGGAGATTTGGGACCGGTCCAGTCCAGAGG TCAAGGAGGCCTATGGCGAGAAGTTTGTTGCAGACTATAAGAAATCAGCTGAACAAATGGAGCAGAAGTG CACACAGGATCTGTCGTTGGTGACCAACTGCATGGAGCATGCGCTGATTGCCTGCCACCCCCGTACTCGC TACTCAGCTGGCTGGGATGCCAAGCTTCTCTACCTCCCCATGAGCTACATGCCCACCTTCCTGGTGGATG CCATTATGTACTGGGTCTCTCCAAGCCCGGCCAAGGCTCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223973 representing NM_003708
Red=Cloning site Green=Tags(s) MWLYLAVFVGLYYLLHWYRERQVLSHLRDKYVFITGCDSGFGKLLARQLDARGLRVLAACLTEKGAEQLR GQTSDRLETVTLDVTKTESVAAAAQWVKECVRDKGLWGLVNNAGISLPTAPNELLTKQDLLTILDVNLLG VIDVTLNLLPLVRRARGRVVNVFSVMGRVSLFGGGYCISKYGVEAFSDSLRRELSYFGVKVAMIEPGYFK TAVTSKERFLKSFLEIWDRSSPEVKEAYGEKFVADYKKSAEQMEQKCTQDLSLVTNCMEHALIACHPRTR YSAGWDAKLLYLPMSYMPTFLVDAIMYWVSPSPAKAL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003708 |
ORF Size | 951 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003708.2, NP_003699.2 |
RefSeq Size | 2425 bp |
RefSeq ORF | 954 bp |
Locus ID | 8608 |
UniProt ID | O75452 |
Domains | adh_short |
Protein Pathways | Metabolic pathways, Retinol metabolism |
Gene Summary | Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol, 9-cis-retinol, 11-cis-retinol and 13-cis-retinol to the corresponding aldehydes (PubMed:10329026, PubMed:12534290, PubMed:9677409). Has higher activity towards CRBP-bound retinol than with free retinol (PubMed:12534290). Oxidizes also 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction (PubMed:10329026, PubMed:9677409, PubMed:29541409).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223973 | RDH16 (Myc-DDK-tagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 3,600.00 |
|
RC223973L1 | Lenti-ORF clone of RDH16 (Myc-DDK-tagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 6,000.00 |
|
RC223973L2 | Lenti-ORF clone of RDH16 (mGFP-tagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 5,890.00 |
|
RC223973L3 | Lenti-ORF clone of RDH16 (Myc-DDK-tagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 5,890.00 |
|
RC223973L4 | Lenti-ORF clone of RDH16 (mGFP-tagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 5,890.00 |
|
SC312933 | RDH16 (untagged)-Human retinol dehydrogenase 16 (all-trans) (RDH16) |
CNY 6,270.00 |
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