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SNRNP200 (NM_014014) Human Tagged ORF Clone
CAT＃: RG223819
- TrueORF®
SNRNP200 (tGFP-tagged) - Human small nuclear ribonucleoprotein 200kDa (U5) (SNRNP200)
ORF Plasmid: DDK
"NM_014014" in other vectors (2)
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CNY 20,900.00
货期*
12周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASCC3L1; BRR2; HELIC2; RP33; U5-200KD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223819 representing NM_014014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGTAACCGCCCGTAGTCTGCAATACGAGTACAAGGCGAACTCGAATCTTGTGCTCCAAGCTG ACCGTTCTCTCATTGACCGGACCCGCCGGGATGAACCCACAGGAGAGGTGCTGTCCCTTGTTGGGAAGCT GGAGGGCACCCGTATGGGAGACAAGGCTCAACGGACCAAACCGCAGATGCAGGAGGAAAGAAGAGCCAAG CGAAGAAAGCGTGATGAGGACCGGCATGACATCAACAAGATGAAGGGTTATACTCTGCTGTCGGAGGGCA TTGATGAGATGGTGGGCATCATCTACAAGCCCAAAACTAAAGAGACTCGGGAGACCTATGAGGTGCTACT CAGCTTCATCCAGGCTGCTCTTGGGGACCAGCCACGTGATATCCTTTGTGGGGCAGCTGATGAAGTTCTA GCTGTTCTAAAGAATGAAAAGCTGCGGGACAAGGAAAGGCGAAAGGAGATTGACCTGCTGCTGGGTCAAA CAGATGATACCAGATACCATGTGCTAGTGAACCTGGGCAAAAAGATCACAGACTATGGTGGAGATAAGGA AATCCAAAATATGGATGACAACATTGATGAGACATACGGTGTGAATGTGCAGTTTGAGTCTGATGAGGAG GAAGGTGATGAAGACGTATACGGGGAGGTTCGAGAAGAGGCATCTGATGATGACATGGAAGGGGACGAGG CTGTCGTGCGCTGCACCCTCTCGGCTAATCTCGTAGCCTCAGGTGAACTGATGAGTTCCAAGAAGAAGGA TTTGCACCCTCGGGATATTGATGCATTTTGGCTGCAGCGGCAGCTCAGTCGTTTCTATGATGATGCCATC GTGTCGCAGAAGAAGGCAGATGAAGTATTGGAGATTTTGAAGACGGCCAGTGATGATCGGGAATGTGAAA ATCAGCTGGTTCTGCTGCTTGGTTTCAACACCTTTGATTTCATTAAAGTGTTGCGGCAGCACAGGATGAT GATTTTATACTGTACCTTGCTGGCCAGTGCACAAAGTGAAGCTGAAAAGGAAAGGATTATGGGAAAGATG GAAGCTGACCCAGAGCTATCCAAGTTCCTCTACCAGCTTCATGAAACCGAGAAGGAGGATCTGATCCGAG AGGAAAGGTCCCGGAGAGAGCGAGTGCGTCAGTCTCGAATGGACACAGATCTGGAAACCATGGATCTCGA CCAGGGTGGAGAGGCACTGGCTCCACGGCAGGTTCTGGACTTGGAGGACCTGGTTTTTACCCAAGGGAGC CACTTTATGGCCAATAAACGCTGTCAGCTTCCTGATGGATCCTTCCGTCGCCAGCGTAAGGGCTATGAAG AGGTGCATGTGCCTGCTCTGAAGCCCAAGCCCTTTGGCTCAGAAGAACAACTGCTTCCAGTGGAAAAGCT GCCAAAGTATGCCCAGGCTGGGTTTGAGGGCTTCAAAACACTGAATCGGATCCAGAGTAAGCTCTACCGT GCTGCCCTTGAGACGGATGAGAATCTGCTGCTGTGTGCTCCTACTGGTGCTGGGAAGACCAACGTGGCCC TGATGTGCATGCTCCGAGAGATTGGGAAACACATAAACATGGACGGCACCATCAATGTGGATGACTTCAA GATTATCTACATTGCCCCCATGCGCTCCTTGGTGCAGGAGATGGTGGGCAGCTTTGGAAAGCGCCTGGCC ACTTATGGCATCACTGTTGCTGAACTGACTGGGGACCACCAGCTGTGCAAAGAAGAGATCAGTGCCACTC AGATCATCGTCTGCACCCCCGAGAAGTGGGACATCATCACCCGCAAGGGTGGTGAGCGCACCTACACCCA GCTGGTGCGGCTCATCATTCTGGATGAGATTCATCTTCTCCACGATGACAGAGGTCCTGTCTTAGAAGCT TTAGTGGCCAGGGCCATCCGAAACATTGAGATGACCCAAGAGGATGTCCGACTCATTGGTCTCAGTGCCA CCCTACCCAACTATGAAGATGTAGCCACCTTTCTACGTGTTGACCCTGCCAAGGGTCTCTTTTACTTTGA CAACAGCTTCCGTCCAGTGCCTCTGGAACAGACATATGTGGGTATCACAGAGAAAAAAGCTATCAAGCGT TTCCAGATCATGAATGAAATCGTCTATGAAAAAATCATGGAACATGCTGGAAAAAATCAGGTGCTGGTGT TTGTCCACTCCCGGAAGGAGACTGGAAAGACAGCCAGGGCCATCCGGGACATGTGCCTAGAAAAGGACAC TCTGGGTCTGTTTCTGAGGGAGGGCTCAGCCTCCACAGAAGTCCTGCGAACAGAAGCTGAGCAGTGCAAG AACCTAGAGCTGAAGGATCTTCTGCCTTATGGCTTTGCTATTCATCACGCAGGCATGACCAGGGTTGACC GAACACTCGTGGAGGATCTTTTTGCTGATAAACATATTCAGGTTTTAGTTTCCACAGCAACTCTAGCTTG GGGTGTGAATCTCCCTGCACATACAGTCATCATCAAAGGCACCCAGGTGTACAGTCCAGAGAAGGGGCGT TGGACAGAACTGGGAGCACTGGACATTCTGCAGATGCTGGGACGTGCCGGAAGACCCCAGTATGACACCA AGGGTGAAGGCATACTCATCACATCTCATGGGGAGCTACAGTACTACCTGTCCCTCCTCAATCAACAACT TCCTATTGAAAGCCAGATGGTTTCAAAGCTTCCTGACATGCTCAATGCAGAAATCGTGCTAGGAAATGTC CAGAATGCCAAGGATGCGGTGAACTGGCTGGGCTATGCCTACCTCTATATCCGAATGCTGCGATCCCCAA CCCTCTATGGCATCTCTCATGATGACCTCAAGGGAGATCCCCTGCTGGACCAGCGCCGACTAGATCTGGT TCATACAGCTGCCCTGATGCTGGACAAGAACAATCTGGTCAAGTACGACAAGAAGACGGGCAACTTCCAG GTGACAGAACTGGGCCGTATAGCCAGCCACTACTACATCACCAATGATACAGTGCAGACTTACAACCAGC TGCTGAAGCCCACCCTGAGTGAGATTGAGCTTTTCAGGGTCTTCTCATTGTCCTCTGAGTTCAAGAACAT CACAGTGAGAGAGGAGGAGAAGCTGGAGCTGCAGAAGTTGCTGGAGAGGGTGCCTATCCCTGTAAAGGAG AGCATTGAGGAACCCAGTGCTAAGATCAACGTTCTTCTGCAAGCCTTCATCTCACAGCTGAAATTGGAGG GCTTTGCACTGATGGCTGACATGGTGTATGTCACACAGTCGGCTGGCCGGTTGATGCGAGCGATATTTGA AATTGTCCTGAACCGAGGTTGGGCACAGCTTACAGACAAGACCCTGAACCTCTGCAAGATGATCGACAAA CGCATGTGGCAGTCCATGTGTCCTCTGCGCCAGTTCCGGAAACTCCCTGAGGAAGTAGTGAAGAAGATTG AGAAGAAGAATTTCCCCTTTGAGCGTCTGTACGACCTGAATCATAATGAGATTGGGGAGCTTATCCGCAT GCCAAAGATGGGGAAGACCATCCACAAATATGTCCATCTGTTTCCCAAGTTGGAGTTGTCAGTGCACCTG CAGCCTATCACACGCTCCACCCTGAAGGTGGAGCTGACCATCACGCCAGACTTCCAGTGGGATGAAAAGG TGCATGGTTCATCCGAGGCTTTTTGGATTCTGGTGGAGGATGTGGACAGCGAGGTGATTCTGCACCATGA GTATTTTCTCCTCAAGGCCAAGTACGCCCAGGACGAGCACCTCATTACATTCTTCGTGCCTGTCTTTGAA CCGCTGCCCCCTCAGTACTTCATCCGAGTGGTGTCTGACCGCTGGCTCTCTTGTGAGACCCAGCTGCCTG TCTCCTTCCGGCACCTGATCTTGCCGGAGAAGTACCCCCCTCCAACCGAACTTTTGGACCTGCAGCCCTT GCCCGTGTCTGCTCTGAGAAACAGTGCCTTTGAGAGTCTTTACCAAGATAAATTTCCTTTCTTCAATCCC ATCCAGACCCAGGTGTTTAACACTGTATACAACAGTGACGACAACGTGTTTGTGGGGGCCCCCACGGGCA GCGGGAAGACTATTTGTGCAGAGTTTGCCATCCTGCGAATGCTGCTGCAGAGCTCGGAGGGGCGCTGTGT GTACATCACCCCCATGGAGGCCCTGGCAGAGCAGGTATACATGGACTGGTACGAGAAGTTCCAGGACAGG CTCAACAAGAAGGTGGTACTCCTGACAGGCGAGACCAGCACAGACCTGAAGCTGCTGGGCAAAGGGAACA TTATCATCAGCACCCCTGAGAAGTGGGACATACTTTCCCGGCGATGGAAGCAGCGCAAGAACGTGCAGAA CATCAACCTCTTCGTGGTGGATGAGGTCCACCTTATCGGGGGCGAGAATGGGCCTGTCTTAGAAGTGATC TGCTCCCGAATGCGCTACATCTCCTCCCAGATTGAGCGGCCCATTCGCATTGTGGCACTCAGCTCTTCGC TCTCCAATGCCAAGGATGTGGCCCACTGGCTGGGCTGCAGTGCCACCTCCACCTTCAACTTCCATCCCAA TGTGCGTCCCGTCCCCTTGGAGCTGCACATCCAGGGCTTCAACATCAGCCATACACAAACCCGCCTGCTC TCCATGGCCAAGCCTGTGTACCATGCTATCACCAAGCACTCGCCCAAGAAGCCTGTCATTGTCTTTGTGC CGTCTCGCAAGCAGACCCGCCTCACTGCCATTGACATCCTCACCACCTGTGCAGCAGACATCCAACGGCA GAGGTTCTTGCACTGCACCGAGAAGGATCTGATTCCGTACCTGGAGAAGCTAAGTGACAGCACGCTCAAG GAAACGCTGCTAAATGGGGTGGGCTACCTGCATGAGGGGCTCAGCCCCATGGAGCGACGCCTGGTGGAGC AGCTCTTCAGCTCAGGGGCTATCCAGGTGGTGGTGGCTTCTCGGAGTCTCTGCTGGGGCATGAACGTGGC TGCCCACCTGGTAATCATCATGGATACCCAGTACTACAATGGCAAGATCCACGCCTATGTGGATTACCCC ATCTATGACGTGCTTCAGATGGTGGGCCACGCCAACCGCCCTTTGCAGGACGATGAGGGGCGCTGTGTCA TCATGTGTCAGGGCTCCAAGAAGGATTTCTTCAAGAAGTTCTTATATGAGCCATTGCCAGTAGAATCTCA CCTGGACCACTGTATGCATGACCACTTCAATGCTGAGATCGTCACCAAGACCATTGAGAACAAGCAGGAT GCTGTGGACTACCTCACCTGGACCTTTCTGTACCGCCGCATGACACAGAACCCCAATTACTACAACCTGC AGGGCATCTCCCATCGTCACTTGTCGGACCACTTGTCAGAGCTGGTGGAGCAGACCCTGAGTGACCTGGA GCAGTCCAAGTGCATCAGCATCGAGGACGAGATGGACGTGGCGCCTCTGAACCTAGGCATGATCGCCGCC TACTATTACATCAACTACACCACCATTGAGCTCTTCAGCATGTCCCTCAATGCCAAGACCAAGGTGCGAG GGCTTATCGAGATCATCTCCAATGCAGCAGAGTATGAGAACATTCCCATCCGGCACCATGAAGACAATCT CCTGAGGCAGTTGGCTCAGAAGGTCCCCCACAAGCTGAATAACCCTAAGTTCAATGATCCGCACGTCAAG ACCAACCTGCTCCTGCAGGCTCACTTGTCTCGCATGCAGCTGAGTGCTGAGTTGCAGTCAGATACGGAGG AAATCCTTAGTAAGGCAATCCGGCTCATCCAGGCCTGCGTGGATGTCCTTTCCAGCAATGGGTGGCTCAG CCCTGCTCTGGCAGCTATGGAACTGGCCCAGATGGTCACCCAAGCCATGTGGTCCAAGGACTCATACCTG AAGCAGCTGCCACACTTCACCTCTGAGCATATCAAACGTTGCACAGACAAGGGAGTGGAGAGTGTTTTCG ACATCATGGAGATGGAGGATGAAGAACGGAACGCGTTGCTTCAGCTGACTGACAGCCAGATTGCAGATGT GGCTCGCTTTTGTAACCGCTACCCTAATATCGAACTATCTTATGAGGTGGTAGATAAGGACAGCATCCGC AGTGGCGGGCCAGTTGTGGTGCTGGTGCAGCTGGAGCGAGAGGAGGAAGTCACAGGCCCTGTCATTGCGC CTCTCTTCCCGCAGAAACGTGAAGAGGGCTGGTGGGTGGTGATTGGAGATGCCAAGTCCAATAGCCTCAT CTCCATCAAGAGGCTGACCTTGCAGCAGAAGGCCAAGGTGAAGTTGGACTTTGTGGCCCCAGCCACTGGT GCCCACAACTACACTCTGTACTTCATGAGTGACGCTTACATGGGATGTGACCAGGAGTACAAATTCAGCG TGGATGTGAAAGAAGCTGAGACAGACAGTGATTCAGAT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223819 representing NM_014014
Red=Cloning site Green=Tags(s) MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAK RRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVL AVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEE EGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAI VSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKM EADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYR AALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLA TYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEA LVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKR FQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCK NLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNV QNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQ VTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKE SIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK RMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHL QPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNP IQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDR LNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVI CSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLL SMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLK ETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQD AVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAA YYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVK TNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYL KQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIR SGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014014 |
| ORF Size | 6408 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014014.5 |
| RefSeq Size | 7184 bp |
| RefSeq ORF | 6411 bp |
| Locus ID | 23020 |
| UniProt ID | O75643 |
| Domains | DEAD, helicase_C, AAA, Sec63 |
| Protein Pathways | Spliceosome |
| Gene Summary | Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. [provided by RefSeq, Mar 2010] |
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