HS2ST1 (NM_012262) Human Tagged ORF Clone
CAT#: RG223657
- TrueORF®
HS2ST1 (tGFP-tagged) - Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1
ORF Plasmid: DDK
"NM_012262" in other vectors (6)
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CNY 5,256.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | dJ604K5.2; NFSRA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223657 representing NM_012262
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTCCTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTCGCGGTGGCGA TGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAGCTAGAAAGGGCTATTGCAAG ACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGATGGCCCTCGGCAAGATGCCACTTTAGATGAG GAAGAGGACATGGTGATCATTTATAACAGAGTTCCCAAAACGGCAAGCACTTCATTTACCAATATCGCCT ATGACCTGTGTGCAAAGAATAAATACCATGTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTC ATTGCAAGATCAGGTGCGCTTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCAT GGACACGTTTCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATTAATGTCATAA GGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGAGATGATTATAGACCAGGGTT ACGGAGACGAAAACAAGGAGACAAAAAGACCTTTGATGAATGTGTAGCAGAAGGTGGCTCAGACTGTGCT CCAGAGAAGCTCTGGCTTCAAATCCCGTTCTTCTGTGGCCATAGCTCCGAATGCTGGAATGTGGGAAGCA GGTGGGCTATGGATCAAGCCAAGTATAACCTAATTAATGAATATTTTCTGGTGGGAGTTACTGAAGAACT TGAAGATTTTATCATGTTATTGGAGGCAGCATTGCCCCGGTTTTTCAGGGGTGCTACTGAACTCTATCGC ACAGGAAAGAAATCTCATCTTAGGAAAACCACAGAGAAGAAACTCCCCACTAAACAAACCATTGCAAAAC TACAGCAATCTGATATTTGGAAAATGGAGAATGAGTTCTATGAATTTGCACTAGAGCAGTTCCAATTCAT CAGAGCCCATGCCGTTCGAGAAAAAGATGGAGACCTCTACATCCTCGCACAAAACTTTTTCTATGAAAAG ATTTACCCTAAGTCGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223657 representing NM_012262
Red=Cloning site Green=Tags(s) MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRSKLERAIARHEVREIEQRHTMDGPRQDATLDE EEDMVIIYNRVPKTASTSFTNIAYDLCAKNKYHVLHINTTKNNPVMSLQDQVRFVKNITSWKEMKPGFYH GHVSYLDFAKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCA PEKLWLQIPFFCGHSSECWNVGSRWAMDQAKYNLINEYFLVGVTEELEDFIMLLEAALPRFFRGATELYR TGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVREKDGDLYILAQNFFYEK IYPKSN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012262 |
ORF Size | 1068 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012262.4 |
RefSeq Size | 6708 bp |
RefSeq ORF | 1071 bp |
Locus ID | 9653 |
UniProt ID | Q7LGA3 |
Domains | HS2ST |
Protein Pathways | Heparan sulfate biosynthesis |
Gene Summary | Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223657 | HS2ST1 (Myc-DDK-tagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1 |
CNY 3,656.00 |
|
RC223657L1 | Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC223657L2 | Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC223657L3 | Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223657L4 | Lenti ORF clone of Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC115469 | HS2ST1 (untagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1 |
CNY 3,656.00 |