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DDX31 (NM_022779) Human Tagged ORF Clone
CAT＃: RG223495
- TrueORF®
DDX31 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1
ORF Plasmid: DDK
"NM_022779" in other vectors (6)
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CNY 7,856.00
CNY 9,690.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PPP1R25 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223495 representing NM_022779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGGATCTCGCTTCTCAGAGGCATTCAGAGAGCTTCCCCAGCGTGAACTCTCGGCCGAATGTAA TTTTGCCAGGCCGCGAGGGACGCAGAGAGGGGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCC TACCCGCCCGGTGCCGCCGTCCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCGTTCCGGACCC GGAAGGGGCGGTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGGCCCAGCAGTCTGCGCACCGG GCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGCAGCCGCCGACGGTTCGCTCTTCGACAACCC CAGGACGTTCTCCAGACGTCCCCCAGCCCAGGCGAGTCGGCAAGCAAAGGCTACGAAAAGAAAATACCAA GCGTCCAGTGAGGCTCCCCCAGCGAAACGGAGGAACGAAACTTCATTTCTCCCAGCCAAGAAAACTAGTG TTAAAGAAACTCAGAGGACTTTTAAGGGGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGTTAG CACAAGTGATAGAAACCAGGAGGAGAGACAGTGCATTAAGACTTCATCACTGTTTAAAAACAACCCTGAC ATTCCAGAACTCCACAGACCTGTGGTAAAGCAGGTGCAAGAAAAAGTGTTTACTTCAGCTGCTTTTCATG AGCTGGGCCTCCACCCACATTTAATTTCCACAATAAATACGGTCTTAAAAATGTCTAGTATGACCAGTGT TCAGAAGCAAAGTATTCCTGTGTTGCTGGAAGGCAGAGATGCTCTCGTGAGATCCCAGACGGGCTCAGGT AAAACTCTTGCCTATTGCATCCCTGTGGTCCAGTCCCTTCAAGCAATGGAGTCAAAAATACAGCGCAGTG ATGGCCCCTATGCCCTGGTGCTCGTGCCAACGAGAGAGCTAGCTCTACAAAGCTTTGACACTGTCCAGAA ACTGCTTAAGCCTTTCACCTGGATTGTGCCTGGAGTGTTAATGGGAGGAGAGAAGAGAAAATCAGAAAAG GCCAGACTCCGCAAAGGAATAAATATCCTTATCTCAACTCCTGGACGCCTGGTGGATCATATAAAATCCA CAAAGAACATTCATTTTAGTCGGCTGCGGTGGTTGGTGTTTGATGAAGCAGACAGAATCTTGGATTTGGG TTTTGAAAAGGACATCACAGTGATACTTAATGCTGTAAATGCTGAATGCCAAAAACGACAGAATGTCTTG CTATCAGCGACACTCACAGAAGGTGTAACGCGGCTAGCTGATATCAGTTTGCATGATCCAGTCAGTATTT CTGTCCTGGACAAGAGCCATGACCAGTTGAACCCAAAGGACAAAGCGGTCCAGGAGGTCTGTCCTCCACC AGCTGGCGACAAGCTGGACAGCTTTGCAATACCAGAGAGTCTCAAGCAGCATGTGACTGTGGTTCCCAGC AAACTGAGGCTTGTCTGCCTAGCGGCCTTCATCCTTCAGAAATGCAAGTTTGAGGAAGACCAGAAGATGG TTGTCTTTTTCTCAAGTTGCGAGCTGGTGGAGTTCCACTACAGCCTCTTCCTACAGACCCTGCTGAGCAG CTCAGGGGCGCCGGCATCAGGGCAGTTGCCATCTGCCTCCATGCGATTAAAATTCCTACGGCTGCATGGC GGCATGGAGCAGGAGGAAAGAACAGCAGTGTTTCAGGAATTTTCACATTCCAGAAGAGGCGTCCTTCTTT GCACGGATGTTGCAGCTCGGGGCTTAGATCTCCCTCAAGTCACGTGGATTGTTCAGTACAACGCTCCATC TTCACCTGCAGAATACATCCACCGGATTGGAAGAACCGCCCGGATTGGCTGCCATGGGAGCAGCCTGCTC ATTTTGGCTCCTTCGGAGGCAGAATATGTCAACTCGTTGGCTTCTCACAAAATCAACGTTTCTGAGATTA AGATGGAAGATATTTTGTGTGTTCTGACAAGAGATGATTGTTTTAAAGGGAAACGATGGGGAGCCCAGAA ATCCCATGCTGTTGGCCCCCAGGAAATCCGAGAGCGAGCCACAGTCTTGCAGACGGTATTTGAAGATTAC GTGCACTCCAGTGAGAGGAGGGTCTCCTGGGCAAAGAAAGCTCTGCAGTCCTTCATCCAAGCCTACGCCA CCTACCCCAGGGAGCTGAAGCACATCTTCCACGTCCGATCCCTCCACCTTGGGCATGTGGCGAAGAGCTT CGGACTAAGAGATGCCCCCAGGAATCTTAGTGCCTTGACTAGAAAGAAGAGGAAAGCACACGTGAAAAGG CCTGACCTTCATAAGAAGACCCAGAGTAAACACAGCCTCGCTGAAATCCTACGTTCGGAATACTCAAGCG GCATGGAGGCCGACATCGCCAAGGTCAAAAAGCAAAACGCACCTGGAGAGCCTGGTGGCCGGCCCCTGCA GCACAGTCTGCAGCCGACACCCTGCTTTGGCCGTGGGAAAACATTAAAATGGAGAAAAACCCAAAAAGGT GTACAGCGGGACAGCAAGACTTCCCAGAAAGTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223495 representing NM_022779
Red=Cloning site Green=Tags(s) MAPDLASQRHSESFPSVNSRPNVILPGREGRREGLPPGGGTRGSLVPTRPVPPSPAPLGTSPYSWSRSGP GRGGGAGSSRVPRGVPGPAVCAPGSLLHHASPTQTMAAADGSLFDNPRTFSRRPPAQASRQAKATKRKYQ ASSEAPPAKRRNETSFLPAKKTSVKETQRTFKGNAQKMFSPKKHSVSTSDRNQEERQCIKTSSLFKNNPD IPELHRPVVKQVQEKVFTSAAFHELGLHPHLISTINTVLKMSSMTSVQKQSIPVLLEGRDALVRSQTGSG KTLAYCIPVVQSLQAMESKIQRSDGPYALVLVPTRELALQSFDTVQKLLKPFTWIVPGVLMGGEKRKSEK ARLRKGINILISTPGRLVDHIKSTKNIHFSRLRWLVFDEADRILDLGFEKDITVILNAVNAECQKRQNVL LSATLTEGVTRLADISLHDPVSISVLDKSHDQLNPKDKAVQEVCPPPAGDKLDSFAIPESLKQHVTVVPS KLRLVCLAAFILQKCKFEEDQKMVVFFSSCELVEFHYSLFLQTLLSSSGAPASGQLPSASMRLKFLRLHG GMEQEERTAVFQEFSHSRRGVLLCTDVAARGLDLPQVTWIVQYNAPSSPAEYIHRIGRTARIGCHGSSLL ILAPSEAEYVNSLASHKINVSEIKMEDILCVLTRDDCFKGKRWGAQKSHAVGPQEIRERATVLQTVFEDY VHSSERRVSWAKKALQSFIQAYATYPRELKHIFHVRSLHLGHVAKSFGLRDAPRNLSALTRKKRKAHVKR PDLHKKTQSKHSLAEILRSEYSSGMEADIAKVKKQNAPGEPGGRPLQHSLQPTPCFGRGKTLKWRKTQKG VQRDSKTSQKV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022779 |
| ORF Size | 2553 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022779.8 |
| RefSeq Size | 3285 bp |
| RefSeq ORF | 2241 bp |
| Locus ID | 64794 |
| UniProt ID | Q9H8H2 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223495 | DDX31 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 |
CNY 6,256.00 |
|
| RC223495L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged |
CNY 8,656.00 |
|
| RC223495L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged |
CNY 10,740.00 |
|
| RC223495L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged |
CNY 10,740.00 |
|
| RC223495L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged |
CNY 10,740.00 |
|
| SC314815 | DDX31 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 |
CNY 16,440.00 |
