GPR44 (PTGDR2) (NM_004778) Human Tagged ORF Clone

CAT#: RG223378

  • TrueORF®

PTGDR2 (tGFP-tagged) - Human G protein-coupled receptor 44 (GPR44)

ORF Plasmid: DDK tGFP



  "NM_004778" in other vectors (4)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal CRTH2 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CD294; CRTH2; DL1R; DP2; GPR44
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG223378 representing NM_004778
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCCAACGCCACACTGAAGCCACTCTGCCCCATCCTGGAGCAGATGAGCCGTCTCCAGAGCCACA
GCAACACCAGCATCCGCTACATCGACCACGCGGCCGTGCTGCTGCACGGGCTGGCCTCGCTGCTGGGCCT
GGTGGAGAATGGAGTCATCCTCTTCGTGGTGGGCTGCCGCATGCGCCAGACCGTGGTCACCACCTGGGTG
CTGCACCTGGCGCTGTCCGACCTGTTGGCCTCTGCTTCCCTGCCCTTCTTCACCTACTTCTTGGCCGTGG
GCCACTCGTGGGAGCTGGGCACCACCTTCTGCAAACTGCACTCCTCCATCTTCTTTCTCAACATGTTCGC
CAGCGGCTTCCTGCTCAGCGCCATCAGCCTGGACCGCTGCCTGCAGGTGGTGCGGCCGGTGTGGGCGCAG
AACCACCGCACCGTGGCCGCGGCGCACAAAGTCTGCCTGGTGCTTTGGGCACTAGCGGTGCTCAACACGG
TGCCCTATTTCGTGTTCCGGGACACCATCTCGCGGCTGGACGGGCGCATTATGTGCTACTACAATGTGCT
GCTCCTGAACCCGGGGCCTGACCGCGATGCCACGTGCAACTCGCGCCAGGCGGCCCTGGCCGTCAGCAAG
TTCCTGCTGGCCTTCCTGGTGCCGCTGGCGATCATCGCCTCGAGCCACGCGGCCGTGAGCCTGCGGTTGC
AGCACCGCGGCCGCCGGCGGCCAGGCCGCTTCGTGCGCCTGGTGGCAGCCGTCGTGGCCGCCTTCGCGCT
CTGCTGGGGGCCCTACCACGTGTTCAGCCTGCTGGAGGCGCGGGCGCACGCAAACCCGGGGCTGCGGCCG
CTCGTGTGGCGCGGGCTGCCCTTCGTCACCAGCCTGGCCTTCTTCAACAGCGTGGCCAACCCGGTGCTCT
ACGTGCTCACCTGCCCCGACATGCTGCGCAAGCTGCGGCGCTCGCTGCGCACGGTGCTGGAGAGCGTGCT
GGTGGACGACAGCGAGCTGGGTGGCGCGGGAAGCAGCCGCCGCCGCCGCACCTCCTCCACCGCCCGCTCG
GCCTCCCCTTTAGCTCTCTGCAGCCGCCCGGAGGAACCGCGGGGCCCCGCGCGTCTCCTCGGCTGGCTGC
TGGGCAGCTGCGCAGCGTCCCCGCAGACGGGCCCCCTGAACCGGGCGCTGAGCAGCACCTCGAGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG223378 representing NM_004778
Red=Cloning site Green=Tags(s)

MSANATLKPLCPILEQMSRLQSHSNTSIRYIDHAAVLLHGLASLLGLVENGVILFVVGCRMRQTVVTTWV
LHLALSDLLASASLPFFTYFLAVGHSWELGTTFCKLHSSIFFLNMFASGFLLSAISLDRCLQVVRPVWAQ
NHRTVAAAHKVCLVLWALAVLNTVPYFVFRDTISRLDGRIMCYYNVLLLNPGPDRDATCNSRQAALAVSK
FLLAFLVPLAIIASSHAAVSLRLQHRGRRRPGRFVRLVAAVVAAFALCWGPYHVFSLLEARAHANPGLRP
LVWRGLPFVTSLAFFNSVANPVLYVLTCPDMLRKLRRSLRTVLESVLVDDSELGGAGSSRRRRTSSTARS
ASPLALCSRPEEPRGPARLLGWLLGSCAASPQTGPLNRALSSTSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004778
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004778.1, NP_004769.1
RefSeq Size 2901 bp
RefSeq ORF 1188 bp
Locus ID 11251
UniProt ID Q9Y5Y4
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Gene Summary This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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