COL8A1 (NM_001850) Human Tagged ORF Clone

CAT＃: RG223322

TrueORF^®

COL8A1 (tGFP-tagged) - Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001850" in other vectors (4)

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CNY 4,370.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	C3orf7
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG223322 representing NM_001850 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCCTGAGTTCCATCAGGC TCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAAATTCCTCCTCAGATGCCACCACA AATTCCACAATACCAGCCCCTGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATG GGCAAGGAGATGCCCCACTTGCAGTATGGCAAAGAGTATCCACACCTACCCCAATATATGAAGGAAATTC AACCGGCGCCAAGAATGGGCAAGGAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTAGCCAGTTTACG AGGGGAACAAGGTCCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGTCAT GGGATACCTGGAATTAAAGGAAAACCAGGGCCACAGGGATATCCAGGAGTTGGAAAGCCAGGTATGCCTG GAATGCCAGGGAAGCCAGGAGCCATGGGCATGCCTGGGGCAAAAGGAGAAATTGGACAGAAAGGGGAAAT TGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCTCATGGACTTCCTGGCATTGGGAAGCCA GGTGGGCCAGGGTTACCAGGGCAACCAGGACCAAAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTC AAGGCCTTCGGGGTCCTAAAGGAGACAAGGGCTTCGGGATGCCAGGTGCGCCAGGTGTAAAGGGGCCTCC AGGGATGCACGGCCCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCAAACCAGGAGTGACAGGCTTCCCT GGGCCCCAGGGCCCCCTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATTGGGGTAC CGGGGGTTCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAGGCCAGGATGGGATCCCAGGCCAGCC AGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCAGGGCTACCAGGACCCCCAGGCCTTCCAGGGATT GGGAAACCAGGCTTCCCAGGACCCAAAGGTGACCGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGACCAA GAGGGGAGAAAGGACCAATAGGTGCCCCAGGAATAGGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAAT CCCAGGTCCTATGGGCCCTCCAGGTGCTATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTGTAGGG CCACAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAGCCAGGTTTCCTTG GTGAAGTAGGGCCTCCTGGCATGAGGGGTTTGCCAGGTCCCATAGGGCCCAAGGGGGAAGCTGGGCAAAA AGGTGTACCAGGACTCCCTGGTGTTCCAGGGCTTCTCGGACCTAAGGGAGAGCCAGGAATCCCAGGGGAT CAGGGTTTACAGGGCCCCCCAGGTATCCCAGGGATTGGGGGCCCTAGTGGCCCCATTGGACCACCTGGGA TTCCAGGCCCCAAAGGGGAGCCGGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGT GGCAGGACTTCATGGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCCTCAAGGCCAGCCTGGCCTTCCAGGA CCCCCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTGTGATGCCCCCTACACCACCACCCCAGGGAGAGT ATCTGCCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTACGGGGCTAAGAAAGGCAA GAATGGAGGGCCAGCCTATGAGATGCCTGCATTTACCGCCGAGCTAACCGCACCTTTCCCACCGGTGGGG GCCCCAGTGAAGTTTAACAAACTGCTGTATAACGGCAGACAGAACTACAACCCGCAGACAGGCATCTTCA CCTGTGAGGCCCCTGGTGTCTACTACTTTGCATACCACGTTCACTGCAAGGGGGGCAACGTGTGGGTTGC TCTATTCAAGAACAACGAGCCCGTGATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCA TCTGGGAGTGCAGTGCTGCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGCCCTCAGAACAGGCTG CAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATATTTATTGTATCCCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG223322 representing NM_001850 Red=Cloning site Green=Tags(s) MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAM GKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGH GIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKP GGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFP GPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGI GKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVG PQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGD QGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPG PPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVG APVKFNKLLYNGRQNYNPQTGIFTCEAPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQA SGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001850
ORF Size	2232 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001850.3, NP_001841.2
RefSeq Size	2676 bp
RefSeq ORF	2235 bp
Locus ID	1295
UniProt ID	P27658
Domains	C1Q, Collagen
Protein Families	Druggable Genome
Gene Summary	This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC223322	COL8A1 (Myc-DDK-tagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,990.00
RC223322L3	Lenti ORF clone of Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC223322L4	Lenti ORF clone of Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
SC109089	COL8A1 (untagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,950.00

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