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Cytokeratin 1 (KRT1) (NM_006121) Human Tagged ORF Clone
CAT＃: RG223146
- TrueORF®
KRT1 (tGFP-tagged) - Human keratin 1 (KRT1)
ORF Plasmid: DDK
"NM_006121" in other vectors (6)
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CNY 8,792.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223146 representing NM_006121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGACAGTTTAGTTCCAGGTCTGGGTACCGAAGTGGAGGGGGCTTCAGCTCTGGCTCTGCTGGGA TCATCAACTACCAGCGCAGGACCACCAGCAGCTCCACACGCCGCAGTGGAGGAGGTGGTGGGAGATTTTC AAGCTGTGGTGGTGGTGGTGGTAGCTTTGGTGCTGGTGGTGGATTTGGAAGTCGGAGTCTTGTTAACCTT GGTGGCAGTAAAAGCATCTCCATAAGTGTGGCTAGAGGAGGTGGACGTGGTAGTGGCTTTGGTGGTGGTT ATGGTGGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGAGGTGGCATTGGGGGTGG TGGCTTTGGTGGTTTTGGCAGTGGTGGTGGTGGTTTTGGTGGAGGTGGCTTTGGGGGTGGTGGATATGGG GGTGGTTATGGTCCTGTCTGCCCTCCTGGTGGCATACAAGAAGTCACTATCAACCAGAGCCTTCTTCAGC CCCTCAATGTGGAGATTGACCCTGAGATCCAAAAGGTGAAGTCTCGAGAAAGGGAGCAAATCAAGTCACT CAACAACCAATTTGCCTCCTTCATTGACAAGGTGAGGTTCCTGGAGCAGCAGAACCAGGTACTGCAAACA AAATGGGAGCTGCTGCAGCAGGTAGATACCTCCACTAGAACCCATAATTTAGAGCCCTACTTTGAGTCAT TCATCAACAATCTCCGAAGGAGAGTGGACCAACTGAAGAGTGATCAATCTCGGTTGGATTCGGAACTGAA GAACATGCAGGACATGGTGGAGGATTACCGGAACAAGTATGAGGATGAAATCAACAAGCGGACAAATGCA GAGAATGAATTTGTGACCATCAAGAAGGATGTGGATGGTGCTTATATGACCAAGGTGGACCTTCAGGCCA AACTTGACAACTTGCAGCAGGAAATTGATTTCCTTACAGCACTCTACCAAGCAGAGTTGTCTCAGATGCA GACTCAAATCAGTGAAACTAATGTCATCCTCTCTATGGACAACAACCGCAGTCTCGACCTGGACAGCATC ATTGCTGAGGTCAAGGCCCAGTACGAGGATATAGCCCAGAAGAGCAAAGCTGAGGCCGAGTCCTTGTACC AGAGCAAGTATGAAGAGCTGCAGATCACTGCTGGCAGACATGGGGATAGTGTGAGAAATTCAAAGATAGA AATTTCTGAGCTGAATCGTGTGATCCAGAGACTTAGATCTGAAATCGACAATGTCAAGAAGCAGATCTCC AACTTGCAGCAGTCCATCAGTGATGCAGAGCAGCGTGGCGAGAATGCCCTCAAGGATGCCAAGAACAAGC TGAATGACCTGGAGGATGCCCTGCAGCAGGCCAAGGAAGACCTGGCCCGCCTGCTGCGCGACTACCAGGA GCTGATGAACACCAAGCTGGCCCTGGATCTGGAGATTGCCACCTACAGGACCCTCCTGGAGGGAGAAGAA AGCAGGATGTCTGGAGAATGTGCCCCGAACGTGAGTGTGTCTGTGAGCACAAGCCACACCACCATCAGTG GAGGTGGCAGCCGAGGAGGTGGCGGCGGTGGCTACGGCTCTGGAGGTAGCAGCTATGGCTCCGGAGGTGG TAGCTATGGTTCTGGAGGTGGCGGCGGCGGCGGCCGTGGCAGCTATGGCTCCGGAGGTAGCAGCTACGGC TCCGGAGGTGGCAGCTATGGCTCTGGAGGTGGCGGCGGCGGCCATGGCAGCTACGGCTCCGGAAGCAGCA GTGGGGGCTACAGAGGTGGCTCTGGAGGCGGCGGCGGCGGCAGCTCTGGCGGCCGGGGCTCTGGCGGCGG GAGCTCTGGAGGCTCCATAGGAGGCCGGGGATCCAGCTCTGGGGGTGTCAAGTCCTCTGGTGGCAGTTCC AGCGTGAGGTTTGTTTCTACCACTTATTCCGGAGTAACCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223146 representing NM_006121
Red=Cloning site Green=Tags(s) MSRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNL GGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYG GGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQT KWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNA ENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSI IAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQIS NLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEE SRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYG SGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSS SVRFVSTTYSGVTR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006121 |
| ORF Size | 1932 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006121.2, NP_006112.2 |
| RefSeq Size | 2507 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 3848 |
| UniProt ID | P04264 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223146 | KRT1 (Myc-DDK-tagged)-Human keratin 1 (KRT1) |
CNY 7,192.00 |
|
| RC223146L1 | Lenti ORF clone of Human keratin 1 (KRT1), Myc-DDK-tagged |
CNY 9,592.00 |
|
| RC223146L2 | Lenti ORF clone of Human keratin 1 (KRT1), mGFP tagged |
CNY 9,592.00 |
|
| RC223146L3 | Lenti ORF clone of Human keratin 1 (KRT1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223146L4 | Lenti ORF clone of Human keratin 1 (KRT1), mGFP tagged |
CNY 6,460.00 |
|
| SC303735 | KRT1 (untagged)-Human keratin 1 (KRT1) |
CNY 7,200.00 |
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