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ATP11B (NM_014616) Human Tagged ORF Clone
CAT＃: RG222814
- TrueORF®
ATP11B (tGFP-tagged) - Human ATPase, class VI, type 11B (ATP11B)
ORF Plasmid: DDK
"NM_014616" in other vectors (6)
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CNY 12,920.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ATPIF; ATPIR |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222814 representing NM_014616
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGCTGGATCCGGCAGCAGCTGGGTTTTGACCCACCACATCAGAGTGACACAAGAACCATCTACG TAGCCAACAGGTTTCCTCAGAATGGCCTTTACACACCTCAGAAATTTATAGATAACAGGATCATTTCATC TAAGTACACTGTGTGGAATTTTGTTCCAAAAAATTTATTTGAACAGTTCAGAAGAGTGGCAAACTTTTAT TTTCTTATTATATTTTTGGTTCAGCTTATGATTGATACACCTACCAGTCCAGTTACCAGTGGACTTCCAT TATTCTTTGTGATAACAGTAACTGCCATAAAGCAGGGATATGAAGATTGGTTACGGCATAACTCAGATAA TGAAGTAAATGGAGCTCCTGTTTATGTTGTTCGAAGTGGTGGCCTTGTAAAAACTAGATCAAAAAACATT CGGGTGGGTGATATTGTTCGAATAGCCAAAGATGAAATTTTTCCTGCAGACTTGGTGCTTCTGTCCTCAG ATCGACTGGATGGTTCCTGTCACGTTACAACTGCTAGTTTGGACGGAGAAACTAACCTGAAGACACATGT GGCAGTTCCAGAAACAGCATTATTACAAACAGTTGCCAATTTGGACACTCTAGTAGCTGTAATAGAATGC CAGCAACCAGAAGCAGACTTATACAGATTCATGGGACGAATGATCATAACCCAACAAATGGAAGAAATTG TAAGACCTCTGGGGCCGGAGAGTCTCCTGCTTCGTGGAGCCAGATTAAAAAACACAAAAGAAATTTTTGG TGTTGCGGTATACACTGGAATGGAAACTAAGATGGCATTAAATTACAAGAGCAAATCACAGAAACGATCT GCAGTAGAAAAGTCAATGAATACATTTTTGATAATTTATCTAGTAATTCTTATATCTGAAGCTGTCATCA GCACTATCTTGAAGTATACATGGCAAGCTGAAGAAAAATGGGATGAACCTTGGTATAACCAAAAAACAGA ACATCAAAGAAATAGCAGTAAGATTCTGAGATTTATTTCAGACTTCCTTGCTTTTTTGGTTCTCTACAAT TTCATCATTCCAATTTCATTATATGTGACAGTCGAAATGCAGAAATTTCTTGGATCATTTTTTATTGGCT GGGATCTTGATCTGTATCATGAAGAATCAGATCAGAAAGCTCAAGTCAATACTTCCGATCTGAATGAAGA GCTTGGACAGGTAGAGTACGTGTTTACAGATAAAACTGGTACACTGACAGAAAATGAGATGCAGTTTCGG GAATGTTCAATTAATGGCATGAAATACCAAGAAATTAATGGTAGACTTGTACCCGAAGGACCAACACCAG ACTCTTCAGAAGGAAACTTATCTTATCTTAGTAGTTTATCCCATCTTAACAACTTATCCCATCTTACAAC CAGTTCCTCTTTCAGAACCAGTCCTGAAAATGAAACTGAACTAATTAAAGAACATGATCTCTTCTTTAAA GCAGTCAGTCTCTGTCACACTGTACAGATTAGCAATGTTCAAACTGACTGCACTGGTGATGGTCCCTGGC AATCCAACCTGGCACCATCGCAGTTGGAGTACTATGCATCTTCACCAGATGAAAAGGCTCTAGTAGAAGC TGCTGCAAGGATTGGTATTGTGTTTATTGGCAATTCTGAAGAAACTATGGAGGTTAAAACTCTTGGAAAA CTGGAACGGTACAAACTGCTTCATATTCTGGAATTTGATTCAGATCGTAGGAGAATGAGTGTAATTGTTC AGGCACCTTCAGGTGAGAAGTTATTATTTGCTAAAGGAGCTGAGTCATCAATTCTCCCTAAATGTATAGG TGGAGAAATAGAAAAAACCAGAATTCATGTAGATGAATTTGCTTTGAAAGGGCTAAGAACTCTGTGTATA GCATATAGAAAATTTACATCAAAAGAGTATGAGGAAATAGATAAACGCATATTTGAAGCCAGGACTGCCT TGCAGCAGCGGGAAGAGAAATTGGCAGCTGTTTTCCAGTTCATAGAGAAAGACCTGATATTACTTGGAGC CACAGCAGTAGAAGACAGACTACAAGATAAAGTTCGAGAAACTATTGAAGCATTGAGAATGGCTGGTATC AAAGTATGGGTACTTACTGGGGATAAACATGAAACAGCTGTTAGTGTGAGTTTATCATGTGGCCATTTTC ATAGAACCATGAACATCCTTGAACTTATAAACCAGAAATCAGACAGCGAGTGTGCTGAACAATTGAGGCA GCTTGCCAGAAGAATTACAGAGGATCATGTGATTCAGCATGGGCTGGTAGTGGATGGGACCAGCCTATCT CTTGCACTCAGGGAGCATGAAAAACTATTTATGGAAGTTTGCAGAAATTGTTCAGCTGTATTATGCTGTC GTATGGCTCCACTGCAGAAAGCAAAAGTAATAAGACTAATAAAAATATCACCTGAGAAACCTATAACATT GGCTGTTGGTGATGGTGCTAATGACGTAAGCATGATACAAGAAGCCCATGTTGGCATAGGAATCATGGGT AAAGAAGGAAGACAGGCTGCAAGAAACAGTGACTATGCAATAGCCAGATTTAAGTTCCTCTCCAAATTGC TTTTTGTTCATGGTCATTTTTATTATATTAGAATAGCTACCCTTGTACAGTATTTTTTTTATAAGAATGT GTGCTTTATCACACCCCAGTTTTTATATCAGTTCTACTGTTTGTTTTCTCAGCAAACATTGTATGACAGC GTGTACCTGACTTTATACAATATTTGTTTTACTTCCCTACCTATTCTGATATATAGTCTTTTGGAACAGC ATGTAGACCCTCATGTGTTACAAAATAAGCCCACCCTTTATCGAGACATTAGTAAAAACCGCCTCTTAAG TATTAAAACATTTCTTTATTGGACCATCCTGGGCTTCAGTCATGCCTTTATTTTCTTTTTTGGATCCTAT TTACTAATAGGGAAAGATACATCTCTGCTTGGAAATGGCCAGATGTTTGGAAACTGGACATTTGGCACTT TGGTCTTCACAGTCATGGTTATTACAGTCACAGTAAAGATGGCTCTGGAAACTCATTTTTGGACTTGGAT CAACCATCTCGTTACCTGGGGATCTATTATATTTTATTTTGTATTTTCCTTGTTTTATGGAGGGATTCTC TGGCCATTTTTGGGCTCCCAGAATATGTATTTTGTGTTTATTCAGCTCCTGTCAAGTGGTTCTGCTTGGT TTGCCATAATCCTCATGGTTGTTACATGTCTATTTCTTGATATCATAAAGAAGGTCTTTGACCGACACCT CCACCCTACAAGTACTGAAAAGGCACAGCTTACTGAAACAAATGCAGGTATCAAGTGCTTGGACTCCATG TGCTGTTTCCCGGAAGGAGAAGCAGCGTGTGCATCTGTTGGAAGAATGCTGGAACGAGTTATAGGAAGAT GTAGTCCAACCCACATCAGCAGATCATGGAGTGCATCGGATCCTTTCTATACCAACGACAGGAGCATCTT GACTCTCTCCACAATGGACTCATCTACTTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222814 representing NM_014616
Red=Cloning site Green=Tags(s) MWRWIRQQLGFDPPHQSDTRTIYVANRFPQNGLYTPQKFIDNRIISSKYTVWNFVPKNLFEQFRRVANFY FLIIFLVQLMIDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHNSDNEVNGAPVYVVRSGGLVKTRSKNI RVGDIVRIAKDEIFPADLVLLSSDRLDGSCHVTTASLDGETNLKTHVAVPETALLQTVANLDTLVAVIEC QQPEADLYRFMGRMIITQQMEEIVRPLGPESLLLRGARLKNTKEIFGVAVYTGMETKMALNYKSKSQKRS AVEKSMNTFLIIYLVILISEAVISTILKYTWQAEEKWDEPWYNQKTEHQRNSSKILRFISDFLAFLVLYN FIIPISLYVTVEMQKFLGSFFIGWDLDLYHEESDQKAQVNTSDLNEELGQVEYVFTDKTGTLTENEMQFR ECSINGMKYQEINGRLVPEGPTPDSSEGNLSYLSSLSHLNNLSHLTTSSSFRTSPENETELIKEHDLFFK AVSLCHTVQISNVQTDCTGDGPWQSNLAPSQLEYYASSPDEKALVEAAARIGIVFIGNSEETMEVKTLGK LERYKLLHILEFDSDRRRMSVIVQAPSGEKLLFAKGAESSILPKCIGGEIEKTRIHVDEFALKGLRTLCI AYRKFTSKEYEEIDKRIFEARTALQQREEKLAAVFQFIEKDLILLGATAVEDRLQDKVRETIEALRMAGI KVWVLTGDKHETAVSVSLSCGHFHRTMNILELINQKSDSECAEQLRQLARRITEDHVIQHGLVVDGTSLS LALREHEKLFMEVCRNCSAVLCCRMAPLQKAKVIRLIKISPEKPITLAVGDGANDVSMIQEAHVGIGIMG KEGRQAARNSDYAIARFKFLSKLLFVHGHFYYIRIATLVQYFFYKNVCFITPQFLYQFYCLFSQQTLYDS VYLTLYNICFTSLPILIYSLLEQHVDPHVLQNKPTLYRDISKNRLLSIKTFLYWTILGFSHAFIFFFGSY LLIGKDTSLLGNGQMFGNWTFGTLVFTVMVITVTVKMALETHFWTWINHLVTWGSIIFYFVFSLFYGGIL WPFLGSQNMYFVFIQLLSSGSAWFAIILMVVTCLFLDIIKKVFDRHLHPTSTEKAQLTETNAGIKCLDSM CCFPEGEAACASVGRMLERVIGRCSPTHISRSWSASDPFYTNDRSILTLSTMDSSTC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014616 |
| ORF Size | 3531 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014616.3 |
| RefSeq Size | 7368 bp |
| RefSeq ORF | 3534 bp |
| Locus ID | 23200 |
| UniProt ID | Q9Y2G3 |
| Protein Families | Transmembrane |
| Gene Summary | P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM, Feb 2005] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222814 | ATP11B (Myc-DDK-tagged)-Human ATPase, class VI, type 11B (ATP11B) |
CNY 8,192.00 |
|
| RC222814L1 | Lenti ORF clone of Human ATPase, class VI, type 11B (ATP11B), Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC222814L2 | Lenti ORF clone of Human ATPase, class VI, type 11B (ATP11B), mGFP tagged |
CNY 13,680.00 |
|
| RC222814L3 | Lenti ORF clone of Human ATPase, class VI, type 11B (ATP11B), Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC222814L4 | Lenti ORF clone of Human ATPase, class VI, type 11B (ATP11B), mGFP tagged |
CNY 13,680.00 |
|
| SC304140 | ATP11B (untagged)-Human ATPase, class VI, type 11B (ATP11B) |
CNY 22,610.00 |
