TFIIS (TCEA2) (NM_003195) Human Tagged ORF Clone
CAT#: RG222661
- TrueORF®
TCEA2 (tGFP-tagged) - Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1
ORF Plasmid: DDK
"NM_003195" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | TFIIS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222661 representing NM_003195
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACCAAGAAGAGCG CGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCCAC CCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAG TCTCTCATCAAGTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCA TGCCTCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCC CAGGGCACCGTCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCG AGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAGAA CCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGT GGGGCCATAACACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGA TCCGTAAGGCCATGACCAAGGAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGA CCTGTTCACCTGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222661 representing NM_003195
Red=Cloning site Green=Tags(s) MMGKEEEIARIARRLDKMVTKKSAEGAMDLLRELKAMPITLHLLQSTRVGMSVNALRKQSSDEEVIALAK SLIKSWKKLLDASDAKARERGRGMPLPTSSRDASEAPDPSRKRPELPRAPSTPRITTFPPVPVTCDAVRN KCREMLTAALQTDHDHVAIGADCERLSAQIEECIFRDVGNTDMKYKNRVRSRISNLKDAKNPDLRRNVLC GAITPQQIAVMTSEEMASDELKEIRKAMTKEAIREHQMARTGGTQTDLFTCGKCRKKNCTYTQVQTRSSD EPMTTFVVCNECGNRWKFC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003195 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003195.6 |
RefSeq Size | 1749 bp |
RefSeq ORF | 900 bp |
Locus ID | 6919 |
UniProt ID | Q15560 |
Domains | TFIIS, TFS2N, TFS2M |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222661 | TCEA2 (Myc-DDK-tagged)-Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1 |
CNY 2,400.00 |
|
RC222661L3 | Lenti ORF clone of Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222661L4 | Lenti ORF clone of Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC109826 | TCEA2 (untagged)-Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1 |
CNY 2,400.00 |