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RHCE (NM_138617) Human Tagged ORF Clone
CAT＃: RG222610
- TrueORF®
RHCE (tGFP-tagged) - Human Rh blood group, CcEe antigens (RHCE), transcript variant 4
ORF Plasmid: DDK
"NM_138617" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CD240CE; RH; Rh4; RH30A; RHC; RHCe(152N); RHE; RhIVb(J); RHIXB; RHNA; RHPI; RhVI; RhVIII |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222610 representing NM_138617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCTAAGTACCCGCGGTCTGTCCGGCGCTGCCTGCCCCTCTGCGCCCTAACACTGGAAGCAGCTC TCATTCTCCTCTTCTATTTTTTTACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATC CTATCAAGTCGGCCAAGATCTGACCGTGATGGCGGCCCTTGGCTTGGGCTTCCTCACCTCAAATTTCCGG AGACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGCAATCCTGC TGGACGGCTTCCTGAGCCAGTTCCCTCCTGGGAAGGTGGTCATCACACTGTTCAGTATTCGGCTGGCCAC CATGAGTGCTATGTCGGTGCTGATCTCAGCGGGTGCTGTCTTGGGGAAGGTCAACTTGGCGCAGTTGGTG GTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCACCCTGAGGATGGTCATCAGTAATATCTTCAACACTT ATGTGCACAGTGCGGTGTTGGCAGGAGGCGTGGCTGTGGGTACCTCGTGTCACCTGATCCCTTCTCCGTG GCTTGCCATGGTGCTGGGTCTTGTGGCTGGGCTGATCTCCATCGGGGGAGCCAAGTGCCTGCCGGTGTGT TGTAACCGAGTGCTGGGGATTCACCACATCTCCGTCATGCACTCCATCTTCAGCTTGCTGGGTCTGCTTG GAGAGATCACCTACATTGTGCTGCTGGTGCTTCATACTGTCTGGAACGGCAATGGCATGTTTGCTCCTAA ATCTCAAAATATGGAAAGCACCTCATGTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222610 representing NM_138617
Red=Cloning site Green=Tags(s) MSSKYPRSVRRCLPLCALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAALGLGFLTSNFR RHSWSSVAFNLFMLALGVQWAILLDGFLSQFPPGKVVITLFSIRLATMSAMSVLISAGAVLGKVNLAQLV VMVLVEVTALGTLRMVISNIFNTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISIGGAKCLPVC CNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVLHTVWNGNGMFAPKSQNMESTSCG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138617 |
| ORF Size | 801 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138617.2, NP_619523.2 |
| RefSeq Size | 1240 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 6006 |
| UniProt ID | P18577 |
| Protein Families | Transmembrane |
| Gene Summary | The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222610 | RHCE (Myc-DDK-tagged)-Human Rh blood group, CcEe antigens (RHCE), transcript variant 4 |
CNY 2,400.00 |
|
| RC222610L3 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222610L4 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| SC309557 | RHCE (untagged)-Human Rh blood group, CcEe antigens (RHCE), transcript variant 4 |
CNY 6,270.00 |
