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Connexin 40.1 (GJD4) (NM_153368) Human Tagged ORF Clone
CAT＃: RG222438
- TrueORF®
GJD4 (tGFP-tagged) - Human gap junction protein, delta 4, 40.1kDa (GJD4)
ORF Plasmid: DDK
"NM_153368" in other vectors (6)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CX40.1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222438 representing NM_153368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGCGTGGACTTGCTAGGGTTTCTCATCATCACATTAAACTGCAACGTGACCATGGTGGGAAAGC TCTGGTTCGTCCTCACGATGCTGCTGCGGATGCTGGTGATTGTCTTGGCGGGGCGACCCGTCTACCAGGA CGAGCAGGAGAGGTTTGTCTGCAACACGCTGCAGCCGGGATGCGCCAATGTTTGCTACGACGTCTTCTCC CCCGTGTCTCACCTGCGGTTCTGGCTGATCCAGGGCGTGTGCGTCCTCCTCCCCTCCGCCGTCTTCAGCG TCTATGTCCTGCACCGAGGAGCCACGCTCGCCGCGCTGGGCCCCCGCCGCTGCCCCGACCCCCGGGAGCC GGCCTCCGGGCAGAGACGCTGCCCGCGGCCATTCGGGGAGCGCGGCGGCCTCCAGGTGCCCGACTTTTCG GCCGGCTACATCATCCACCTCCTCCTCCGGACCCTGCTGGAGGCAGCCTTCGGGGCCTTGCACTACTTTC TCTTTGGATTCCTGGCCCCGAAGAAGTTCCCTTGCACGCGCCCTCCGTGCACGGGCGTGGTGGACTGCTA CGTGTCGCGGCCCACAGAGAAGTCCCTGCTGATGCTGTTCCTCTGGGCGGTCGGCGCGCTGTCTTTTCTG CTGGGCCTCGCCGACCTGGTCTGCAGCCTGCGGCGGCGGATGCGCAGGAGGCCGGGACCCCCCACAAGCC CCTCCATCCGGAAGCAGAGCGGAGCCTCAGGCCACGCGGAGGGACGCCGGACTGACGAGGAGGGTGGGCG GGAGGAAGAGGGGGCACCGGCGCCCCCGGGTGCACGCGCCGGAGGGGAGGGGGCTGGCAGCCCCAGGCGT ACATCCAGGGTGTCAGGGCACACGAAGATTCCGGATGAGGATGAGAGTGAGGTGACATCCTCCGCCAGCG AAAAGCTGGGCAGACAGCCCCGGGGCAGGCCCCACCGAGAGGCCGCCCAGGACCCCAGGGGCTCAGGATC CGAGGAGCAGCCCTCAGCAGCCCCCAGCCGCCTGGCCGCGCCCCCTTCCTGCAGCAGCCTGCAGCCCCCT GACCCGCCTGCCAGCTCCAGTGGTGCTCCCCACCTGAGAGCCAGGAAGTCTGAGTGGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222438 representing NM_153368
Red=Cloning site Green=Tags(s) MEGVDLLGFLIITLNCNVTMVGKLWFVLTMLLRMLVIVLAGRPVYQDEQERFVCNTLQPGCANVCYDVFS PVSHLRFWLIQGVCVLLPSAVFSVYVLHRGATLAALGPRRCPDPREPASGQRRCPRPFGERGGLQVPDFS AGYIIHLLLRTLLEAAFGALHYFLFGFLAPKKFPCTRPPCTGVVDCYVSRPTEKSLLMLFLWAVGALSFL LGLADLVCSLRRRMRRRPGPPTSPSIRKQSGASGHAEGRRTDEEGGREEEGAPAPPGARAGGEGAGSPRR TSRVSGHTKIPDEDESEVTSSASEKLGRQPRGRPHREAAQDPRGSGSEEQPSAAPSRLAAPPSCSSLQPP DPPASSSGAPHLRARKSEWV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153368 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153368.1, NP_699199.1 |
| RefSeq Size | 1580 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 219770 |
| UniProt ID | Q96KN9 |
| Protein Families | Transmembrane |
| Gene Summary | Connexins, such as GJD4, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
S-nitrosylation of connexin43 hemichannels elicits cardiac stress–induced arrhythmias in Duchenne muscular dystrophy mice
,null,
JCI Insight
,PubMed ID 31751316
[GJD4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222438 | GJD4 (Myc-DDK-tagged)-Human gap junction protein, delta 4, 40.1kDa (GJD4) |
CNY 3,656.00 |
|
| RC222438L1 | Lenti ORF clone of Human gap junction protein, delta 4, 40.1kDa (GJD4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222438L2 | Lenti ORF clone of Human gap junction protein, delta 4, 40.1kDa (GJD4), mGFP tagged |
CNY 5,890.00 |
|
| RC222438L3 | Lenti ORF clone of Human gap junction protein, delta 4, 40.1kDa (GJD4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222438L4 | Lenti ORF clone of Human gap junction protein, delta 4, 40.1kDa (GJD4), mGFP tagged |
CNY 5,890.00 |
|
| SC306596 | GJD4 (untagged)-Human gap junction protein, delta 4, 40.1kDa (GJD4) |
CNY 6,080.00 |
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