ACOT11 (NM_015547) Human Tagged ORF Clone
CAT#: RG222250
- TrueORF®
ACOT11 (tGFP-tagged) - Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1
ORF Plasmid: DDK
"NM_015547" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BFIT; STARD14; THEA; THEM1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222250 representing NM_015547
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCAGAATGTCGGAAATCACCTGCGACGGGGCTTGGCCTCTGTGTTCTCCAACCGCACATCCCGGA AGTCAGCCTTACGTGCGGGGAACGACAGTGCCATGGCAGACGGCGAGGGATACCGGAACCCCACGGAGGT GCAGATGAGCCAGCTGGTGCTGCCCTGCCACACCAACCAACGTGGTGAGCTGAGCGTCGGGCAGCTGCTC AAGTGGATTGACACCACGGCTTGCCTGTCCGCGGAGAGGCACGCTGGCTGCCCCTGTGTCACAGCTTCCA TGGATGACATCTATTTTGAGCACACCATTAGTGTTGGACAAGTGGTGAATATCAAGGCCAAGGTGAACCG GGCCTTCAACTCCAGCATGGAGGTGGGCATCCAGGTGGCCTCGGAGGACCTGTGCTCTGAGAAGCAGTGG AATGTGTGCAAGGCCTTGGCCACCTTCGTGGCCCGCCGAGAGATCACCAAGGTGAAGCTGAAGCAGATCA CGCCGCGGACAGAAGAGGAGAAGATGGAGCACAGTGTGGCGGCTGAGCGCCGGCGCATGCGCCTTGTCTA TGCAGACACCATCAAGGACCTCCTGGCCAACTGCGCCATTCAGGGCGATCTGGAGAGCAGAGACTGTAGC CGCATGGTGCCGGCTGAGAAGACCCGTGTGGAGAGTGTGGAGCTGGTCCTGCCTCCCCACGCCAATCACC AGGGCAACACCTTTGGGGGCCAGATCATGGCCTGGATGGAGAATGTGGCCACCATTGCAGCCAGCCGGCT CTGCCGTGCCCACCCTACGCTGAAGGCCATTGAAATGTTCCACTTCCGAGGCCCGTCCCAGGTCGGCGAC CGTCTGGTGCTCAAAGCCATCGTGAACAATGCCTTCAAACATAGCATGGAGGTGGGCGTGTGCGTGGAGG CCTATCGCCAGGAGGCTGAGACCCACCGGCGCCACATCAACAGTGCCTTTATGACCTTTGTGGTCCTGGA CGCAGATGACCAGCCCCAGTTGCTGCCCTGGATTCGGCCCCAGCCCGGCGATGGTGAGCGGCGGTACCGA GAGGCCAGTGCCAGAAAGAAGATCCGCCTGGACAGGAAGTACATCGTGTCCTGTAAGCAGACAGAGGTGC CCCTCTCCGTCCCCTGGGACCCTAGCAACCAGGTGTACCTGAGCTACAATAACGTCTCCTCCTTGAAGAT GCTTGTGGCCAAGGACAACTGGGTGCTGTCCTCGGAGATCAGTCAGGTCCGCCTGTACACTCTGGAGGAT GACAAGTTCCTCTCCTTCCACATGGAGATGGTGGTGCATGTGGATGCAGCCCAGGCCTTCCTGCTGCTCT CGGACCTGCGTCAGAGGCCAGAGTGGGACAAGCACTACCGGAGCGTGGAGCTAGTGCAGCAGGTAGACGA GGACGACGCCATCTACCACGTCACCAGCCCTGCCCTCGGAGGTCACACAAAGCCCCAGGACTTCGTGATC CTGGCCTCGAGGCGGAAGCCTTGTGACAATGGGGACCCCTATGTCATCGCGCTGAGGTCGGTCACGCTGC CCACACACCGAGAGACGCCAGAGTACAGACGCGGAGAGACCCTCTGCTCAGGCTTCTGCCTCTGGCGCGA GGGGGACCAGCTGACCAAGTGCTGCTGGGTTAGGGTCTCCCTGACTGAGCTGGTCTCGGCAAGTGGCTTC TATTCCTGGGGGCTCGAATCCAGGTCAAAGGGTCGCAGGAGCGACGGTTGGAATGGAAAACTAGCTGGAG GACACCTGAGTACTCTTAAAGCAATCCCCGTGGCCAAAATCAACAGCCGATTTGGATACCTTCAAGACAC C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222250 representing NM_015547
Red=Cloning site Green=Tags(s) MIQNVGNHLRRGLASVFSNRTSRKSALRAGNDSAMADGEGYRNPTEVQMSQLVLPCHTNQRGELSVGQLL KWIDTTACLSAERHAGCPCVTASMDDIYFEHTISVGQVVNIKAKVNRAFNSSMEVGIQVASEDLCSEKQW NVCKALATFVARREITKVKLKQITPRTEEEKMEHSVAAERRRMRLVYADTIKDLLANCAIQGDLESRDCS RMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAASRLCRAHPTLKAIEMFHFRGPSQVGD RLVLKAIVNNAFKHSMEVGVCVEAYRQEAETHRRHINSAFMTFVVLDADDQPQLLPWIRPQPGDGERRYR EASARKKIRLDRKYIVSCKQTEVPLSVPWDPSNQVYLSYNNVSSLKMLVAKDNWVLSSEISQVRLYTLED DKFLSFHMEMVVHVDAAQAFLLLSDLRQRPEWDKHYRSVELVQQVDEDDAIYHVTSPALGGHTKPQDFVI LASRRKPCDNGDPYVIALRSVTLPTHRETPEYRRGETLCSGFCLWREGDQLTKCCWVRVSLTELVSASGF YSWGLESRSKGRRSDGWNGKLAGGHLSTLKAIPVAKINSRFGYLQDT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015547 |
ORF Size | 1821 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015547.4 |
RefSeq Size | 1921 bp |
RefSeq ORF | 1824 bp |
Locus ID | 26027 |
UniProt ID | Q8WXI4 |
Gene Summary | This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222250 | ACOT11 (Myc-DDK-tagged)-Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1 |
CNY 4,520.00 |
|
RC222250L3 | Lenti ORF clone of Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC222250L4 | Lenti ORF clone of Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
SC304326 | ACOT11 (untagged)-Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 1 |
CNY 4,528.00 |