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UNC13A (NM_001080421) Human Tagged ORF Clone
CAT＃: RG222101
- TrueORF®
UNC13A (tGFP-tagged) - Human unc-13 homolog A (C. elegans) (UNC13A)
ORF Plasmid: DDK
"NM_001080421" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Munc13-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222101 representing NM_001080421
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGGCGGGGGCACGTGCACCATTTCAGTGCAGGTGAGGGTCTGCACAGGAGGAAACCTGCTTGGGG GCCGCCGTCGCCCCTTGTCCCCCTGCAACGAGGAGGTGTGGGGCGAGGCGCCCCTTCCCGGAGCCAGGCC CTTTAACGCCCCCCCAGCCCGCAGCGCCCCCGCCCCATCAGGCGTCCCTGGGCTCCGCCTGCCCGCCCCG CATCAGCGCCACAGCCGGCCCGGCCGCCTCCGGCCCACCTGCGAGCTCGGAGACATGTCTCTGCTTTGCG TTGGAGTCAAAAAAGCCAAGTTTGATGGTGCCCAAGAGAAATTCAACACGTACGTGACCCTGAAAGTGCA GAATGTCAAGAGCACGACCATCGCGGTGCGGGGCAGCCAGCCCAGCTGGGAGCAGGATTTCATGTTCGAG ATTAACCGTCTGGATTTGGGACTGACGGTGGAGGTGTGGAATAAGGGTCTCATCTGGGACACAATGGTGG GCACTGTGTGGATCCCACTGAGGACCATCCGCCAGTCCAATGAGGAGGGCCCTGGAGAGTGGCTGACGCT GGACTCCCAGGTCATCATGGCAGACAGTGAGATCTGTGGCACCAAGGACCCCACCTTCCACCGCATCCTC CTGGACACGCGCTTTGAGCTACCCTTAGACATTCCTGAAGAGGAGGCTCGCTACTGGGCCAAGAAGCTGG AGCAGCTCAATGCTATGCGGGACCAGGATGAATATTCGTTCCAAGATGAGCAAGACAAGCCTCTGCCTGT CCCCAGCAACCAGTGCTGCAACTGGAATTATTTTGGCTGGGGTGAGCAGCACAACGATGACCCCGACAGT GCAGTGGATGATCGTGACAGTGACTACCGCAGTGAAACGAGCAACAGCATCCCGCCGCCCTATTATACTA CGTCACAACCCAACGCCTCAGTCCACCAATATTCTGTTCGCCCACCACCCCTGGGCTCCCGGGAGTCCTA CAGTGACTCCATGCACAGTTACGAGGAGTTCTCTGAGCCACAAGCCCTCAGCCCCACGGGTAGCAGCCGC TATGCCTCTTCCGGGGAGCTGAGCCAGGGAAGCTCTCAGCTGAGCGAGGACTTCGACCCTGACGAGCACA GCCTGCAGGGCTCCGACATGGAGGATGAGCGGGACCGGGACTCCTACCACTCCTGCCACAGCTCGGTCAG CTACCACAAAGACTCGCCTCGCTGGGACCAGGATGAGGAAGAGCTGGAGGAGGACCTGGAGGACTTCCTG GAGGAGGAGGAGCTGCCTGAAGATGAGGAGGAGCTGGAGGAGGAGGAGGAGGAGGTGCCTGACGATTTGG GCAGCTATGCCCAGCGTGAAGACGTAGCTGTGGCTGAGCCCAAAGACTTCAAACGCATCAGCCTCCCGCC AGCTGCCCCAGGGAAGGAGGACAAGGCCCCAGTGGCACCCACCGAGGCCCCCGACATGGCCAAGGTGGCC CCCAAGCCAGCCACGCCCGACAAGGTGCCTGCAGCTGAGCAGATCCCTGAGGCTGAGCCACCCAAGGACG AGGAGAGTTTCAGGCCGAGAGAGGATGAGGAAGGCCAGGAGGGGCAGGACTCCATGTCCAGGGCCAAGGC CAACTGGCTGCGTGCCTTCAACAAGGTGCGGATGCAGCTGCAGGAGGCCCGGGGAGAAGGAGAGATGTCT AAATCCCTATGGTTCAAAGGCGGCCCAGGGGGCGGTCTCATCATCATCGACAGCATGCCAGACATCCGCA AGAGGAAACCTATCCCACTCGTGAGCGACTTGGCCATGTCCCTGGTCCAGTCCAGGAAAGCGGGCATCAC CTCGGCCTTGGCCTCCAGCACGTTGAACAACGAGGAGCTGAAAAACCACGTTTACAAGAAGACCCTGCAA GCCTTAATCTACCCCATCTCGTGCACGACGCCACACAACTTCGAAGTGTGGACGGCCACCACGCCCACCT ACTGCTACGAGTGCGAGGGGCTGCTGTGGGGCATCGCGAGGCAGGGCATGCGCTGCACCGAGTGCGGTGT CAAGTGCCACGAGAAGTGCCAGGACCTGCTCAACGCCGACTGCCTGCAGCGGGCTGCGGAGAAGAGCTCC AAGCACGGGGCGGAGGACCGGACACAGAACATCATCATGGTGCTCAAGGACCGCATGAAGATCCGGGAGC GCAACAAGCCCGAGATCTTCGAGCTCATCCAGGAGATCTTCGCGGTGACCAAGACGGCGCACACGCAGCA GATGAAGGCGGTCAAGCAGAGCGTGCTGGACGGCACGTCCAAGTGGTCCGCCAAGATCAGCATCACCGTG GTCTGCGCCCAGGGCTTGCAGGCAAAGGACAAGACAGGATCCAGTGACCCCTATGTCACCGTCCAGGTCG GGAAGACCAAGAAACGGACAAAAACCATCTATGGGAACCTCAACCCGGTGTGGGAGGAGAATTTCCACTT TGAATGTCACAATTCCTCCGACCGCATCAAGGTGCGCGTCTGGGACGAGGATGACGACATCAAATCCCGC GTGAAACAGAGGTTCAAGAGGGAATCTGACGATTTCCTGGGGCAGACGATCATTGAGGTGCGGACGCTCA GCGGCGAGATGGACGTGTGGTACAACCTGGACAAGCGAACTGACAAATCTGCCGTGTCGGGTGCCATCCG GCTCCACATCAGTGTGGAGATCAAAGGCGAGGAGAAGGTGGCCCCGTACCATGTCCAGTACACCTGTCTG CATGAGAACCTGTTCCACTTCGTGACCGACGTGCAGAACAATGGGGTCGTGAAGATCCCAGATGCCAAGG GTGACGATGCCTGGAAGGTTTACTACGATGAGACAGCCCAGGAGATTGTGGACGAGTTTGCCATGCGCTA CGGCGTCGAGTCCATCTACCAAGCCATGACCCACTTTGCCTGCCTCTCCTCCAAGTATATGTGCCCAGGG GTGCCTGCCGTCATGAGCACCCTGCTCGCCAACATCAATGCCTACTACGCACACACCACCGCCTCCACCA ACGTGTCTGCCTCCGACCGCTTCGCCGCCTCCAACTTTGGGAAAGAGCGCTTCGTGAAACTCCTGGACCA GCTGCATAACTCCCTGCGGATTGACCTCTCCATGTACCGGAATAACTTCCCAGCCAGCAGCCCGGAGAGA CTCCAGGACCTCAAATCCACTGTGGACCTTCTCACCAGCATCACCTTCTTTCGGATGAAGGTACAAGAAC TCCAGAGCCCGCCCCGAGCCAGCCAGGTGGTAAAGGACTGTGTGAAAGCCTGCCTTAATTCTACCTACGA GTACATCTTCAATAACTGCCATGAACTGTACAGCCGGGAGTACCAGACAGACCCGGCCAAGAAGGGGGAA GTTCTCCCAGAGGAACAGGGGCCCAGCATCAAGAACCTCGACTTCTGGTCCAAGCTGATTACCCTCATAG TGTCCATCATTGAGGAAGACAAGAATTCCTACACTCCCTGCCTCAACCAGTTTCCCCAGGAGCTGAATGT GGGTAAAATCAGCGCTGAAGTGATGTGGAATCTGTTTGCCCAAGACATGAAGTACGCCATGGAGGAGCAC GACAAGCATCGTCTATGCAAGAGTGCCGACTACATGAACCTCCACTTCAAGGTGAAATGGCTCTACAATG AGTATGTGACGGAACTTCCCGCCTTCAAGGACCGCGTGCCTGAGTACCCTGCATGGTTTGAACCCTTCGT CATCCAGTGGCTGGATGAGAATGAGGAGGTGTCCCGGGATTTCCTGCACGGTGCCCTGGAGCGAGACAAG AAGGATGGGTTCCAGCAGACCTCAGAGCATGCCCTATTCTCCTGCTCCGTGGTGGATGTTTTCTCCCAAC TCAACCAGAGCTTTGAAATCATCAAGAAACTCGAGTGTCCCGACCCTCAGATCGTGGGGCACTACATGAG GCGCTTTGCCAAGACCATCAGTAATGTGCTCCTCCAGTATGCAGACATCATCTCCAAGGACTTTGCCTCC TACTGCTCCAAGGAGAAGGAGAAAGTGCCCTGCATTCTCATGAATAACACTCAACAGCTACGAGTTCAGC TGGAGAAGATGTTCGAAGCCATGGGAGGAAAGGAGCTGGATGCTGAAGCCAGTGACATCCTGAAGGAGCT TCAGGTGAAACTCAATAACGTCTTGGATGAGCTCAGCCGGGTGTTTGCTACCAGCTTCCAGCCGCACATT GAAGAGTGTGTCAAACAGATGGGTGACATCCTTAGCCAGGTTAAGGGCACAGGCAATGTGCCAGCCAGTG CCTGCAGCAGCGTGGCCCAGGACGCGGACAATGTGTTGCAGCCCATCATGGACCTGCTGGACAGCAACCT GACCCTCTTTGCCAAAATCTGTGAGAAGACTGTGCTGAAGCGAGTGCTGAAGGAGCTGTGGAAGCTGGTT ATGAACACCATGGAGAAAACCATCGTCCTGCCGCCCCTCACTGACCAGACGATGATCGGGAACCTCTTGA GAAAACATGGCAAGGGATTAGAAAAGGGCAGGGTGAAATTGCCAAGCCACTCAGACGGAACCCAGATGAT CTTCAATGCAGCCAAGGAGCTGGGTCAGCTGTCCAAACTCAAGGATCACATGGTACGAGAAGAAGCCAAG AGCTTGACCCCAAAGCAGTGCGCGGTTGTTGAGTTGGCCCTGGACACCATCAAGCAATATTTCCACGCGG GTGGCGTGGGCCTCAAGAAGACCTTCCTGGAGAAGAGCCCGGACCTGCAATCCTTGCGCTATGCCCTGTC GCTCTACACGCAGGCCACCGACCTGCTAATCAAGACCTTTGTACAGACGCAATCGGCCCAGGGCTTGGGT GTAGAAGACCCTGTGGGTGAAGTCTCTGTCCATGTTGAGCTGTTCACTCATCCAGGAACTGGGGAACACA AGGTCACAGTGAAAGTGGTGGCTGCCAATGACCTCAAGTGGCAGACTTCTGGCATCTTCCGGCCGTTCAT CGAGGTCAACATCATTGGGCCCCAGCTCAGCGACAAGAAACGCAAGTTTGCGACCAAATCCAAGAACAAT AGCTGGGCTCCCAAGTACAATGAGAGCTTCCAGTTCACGCTGAGCGCCGACGCGGGTCCCGAGTGCTATG AGCTGCAGGTGTGCGTCAAGGACTACTGCTTCGCGCGCGAGGACCGCACGGTGGGGCTGGCCGTGCTGCA GCTGCGTGAGCTGGCCCAGCGCGGGAGCGCCGCCTGCTGGCTGCCGCTCGGCCGCCGCATCCACATGGAC GACACGGGCCTCACGGTGCTGCGAATCCTCTCGCAGCGCAGCAACGACGAGGTGGCCAAGGAGTTCGTGA AGCTCAAGTCGGACACGCGCTCCGCCGAGGAGGGCGGTGCCGCGCCTGCGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222101 representing NM_001080421
Red=Cloning site Green=Tags(s) MGGGGTCTISVQVRVCTGGNLLGGRRRPLSPCNEEVWGEAPLPGARPFNAPPARSAPAPSGVPGLRLPAP HQRHSRPGRLRPTCELGDMSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFE INRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQVIMADSEICGTKDPTFHRIL LDTRFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDEQDKPLPVPSNQCCNWNYFGWGEQHNDDPDS AVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPQALSPTGSSR YASSGELSQGSSQLSEDFDPDEHSLQGSDMEDERDRDSYHSCHSSVSYHKDSPRWDQDEEELEEDLEDFL EEEELPEDEEELEEEEEEVPDDLGSYAQREDVAVAEPKDFKRISLPPAAPGKEDKAPVAPTEAPDMAKVA PKPATPDKVPAAEQIPEAEPPKDEESFRPREDEEGQEGQDSMSRAKANWLRAFNKVRMQLQEARGEGEMS KSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQ ALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSS KHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITV VCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSR VKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCL HENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPG VPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPER LQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYSREYQTDPAKKGE VLPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYAMEEH DKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDK KDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIISKDFAS YCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASDILKELQVKLNNVLDELSRVFATSFQPHI EECVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLV MNTMEKTIVLPPLTDQTMIGNLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAK SLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGLG VEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNIIGPQLSDKKRKFATKSKNN SWAPKYNESFQFTLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMD DTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080421 |
| ORF Size | 5373 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080421.1, NP_001073890.1 |
| RefSeq Size | 10102 bp |
| RefSeq ORF | 5112 bp |
| Locus ID | 23025 |
| UniProt ID | Q9UPW8 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the UNC13 family. UNC13 proteins bind to phorbol esters and diacylglycerol and play important roles in neurotransmitter release at synapses. Single nucleotide polymorphisms in this gene may be associated with sporadic amyotrophic lateral sclerosis. [provided by RefSeq, Feb 2012] |
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