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DORFIN (RNF19A) (NM_015435) Human Tagged ORF Clone
CAT＃: RG222087
- TrueORF®
RNF19A (tGFP-tagged) - Human ring finger protein 19A (RNF19A), transcript variant 2
ORF Plasmid: DDK
"NM_015435" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RNF19 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222087 representing NM_015435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGAACAAGAAATAGGTTTTATCTCTAAATATAATGAAGGGCTGTGTGTAAACACTGACCCTGTCT CAATTCTAACAAGCATTTTAGACATGAGTTTACATCGGCAAATGGGTTCAGATCGAGATCTTCAGTCCTC TGCTTCATCTGTGAGCTTGCCTTCAGTCAAAAAGGCACCCAAAAAAAGAAGAATTTCAATAGGCTCCCTG TTTCGGAGGAAAAAAGATAACAAACGTAAATCAAGGGAGCTAAATGGCGGGGTGGATGGAATTGCAAGTA TTGAAAGTATACATTCTGAAATGTGTACTGATAAGAACTCCATTTTCTCTACAAATACCTCTTCTGACAA TGGATTAACTTCCATCAGCAAACAAATTGGAGACTTCATAGAGTGCCCTTTGTGCCTTTTGCGGCATTCT AAAGACAGATTTCCTGATATAATGACTTGTCATCACAGATCTTGTGTGGATTGCTTACGACAATATTTAA GGATAGAAATCTCTGAAAGCAGAGTTAATATTAGTTGCCCAGAATGTACTGAACGGTTTAATCCCCATGA TATTCGCTTGATATTAAGTGATGATGTCTTGATGGAAAAATACGAAGAATTTATGCTTAGACGGTGGCTT GTTGCAGATCCTGATTGTAGGTGGTGTCCAGCTCCAGACTGTGGATATGCTGTGATAGCATTTGGATGTG CCAGCTGTCCAAAATTAACTTGTGGGCGAGAGGGCTGTGGAACAGAGTTTTGCTACCACTGTAAACAGAT TTGGCACCCCAACCAGACCTGTGATGCTGCTCGACAAGAGAGAGCCCAGAGCTTACGTTTGAGAACTATA CGTTCTTCATCCATTAGTTATAGTCAAGAGTCTGGAGCAGCAGCTGATGATATAAAGCCATGTCCACGAT GTGCTGCTTATATAATAAAGATGAATGATGGGAGCTGCAATCACATGACATGTGCTGTTTGTGGTTGTGA GTTTTGTTGGTTGTGTATGAAAGAAATCTCAGATTTGCATTATCTAAGTCCATCAGGATGTACTTTTTGG GGGAAGAAACCCTGGAGCCGAAAGAAGAAAATATTGTGGCAACTGGGAACACTGGTTGGTGCTCCTGTCG GAATCGCTTTAATAGCTGGCATTGCTATTCCTGCAATGATTATTGGCATTCCTGTGTATGTGGGCCGCAA GATTCACAATCGCTATGAAGGCAAGGATGTTTCAAAGCACAAACGGAATTTGGCCATAGCAGGTGGTGTA ACGTTGTCTGTAATCGTGTCTCCAGTAGTAGCTGCAGTGACTGTAGGTATCGGTGTTCCTATTATGTTAG CTTATGTCTATGGCGTAGTTCCAATTTCTCTTTGTCGAAGCGGAGGTTGTGGAGTCTCAGCAGGCAATGG AAAAGGAGTTAGGATTGAATTTGATGATGAAAATGATATAAATGTTGGTGGAACTAACACAGCTGTAGAC ACAACATCAGTAGCAGAAGCAAGACACAACCCAAGCATAGGGGAGGGAAGTGTTGGTGGGCTGACTGGCA GTTTGAGTGCAAGTGGAAGCCACATGGATCGAATAGGAGCCATCCGAGACAACCTGAGTGAAACGGCCAG CACCATGGCACTAGCTGGAGCCAGTATAACGGGGAGTCTGTCAGGAAGTGCCATGGTAAACTGTTTTAAC AGGTTGGAAGTACAAGCAGATGTACAGAAAGAACGGTACAGTCTAAGTGGAGAATCTGGCACAGTCAGCT TGGGAACAGTTAGTGATAATGCCAGCACCAAAGCAATGGCAGGATCCATTCTGAATTCCTACATCCCATT GGACAAAGAAGGCAACAGTATGGAGGTGCAAGTAGATATTGAGTCAAAGCCATCCAAATTCAGGCACAAC AGTGGAAGCAGTAGTGTGGATGATGGCAGTGCCACCCGAAGTCATGCTGGCGGTTCATCCAGTGGCTTGC CTGAAGGTAAATCTAGTGCCACCAAGTGGTCCAAAGAAGCAACAGCAGGGAAAAAATCAAAAAGTGGTAA ACTGAGGAAAAAGGGTAACATGAAGATAAATGAGACGAGAGAGGACATGGATGCACAGTTGTTAGAACAA CAAAGCACGAACTCAAGTGAATTTGAGGCTCCATCCCTCAGTGACAGTATGCCTTCTGTAGCAGATTCTC ACTCTAGTCATTTTTCTGAATTTAGTTGTTCTGACCTAGAAAGCATGAAAACTTCTTGTAGTCATGGTTC CAGTGATTATCACACCCGCTTTGCTACTGTTAACATTCTTCCTGAGGTAGAAAATGACCGTCTGGAAAAT TCCCCACATCAGTGTAGCATTTCTGTGGTTACCCAAACTGCTTCCTGTTCAGAAGTTTCACAGTTGAATC ATATTGCTGAAGAACATGGTAACAATGGAATAAAACCTAATGTTGATTTATATTTTGGCGATGCACTAAA AGAAACAAATAACAACCACTCACATCAGACAATGGAATTAAAAGTTGCAATTCAGACTGAAATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222087 representing NM_015435
Red=Cloning site Green=Tags(s) MQEQEIGFISKYNEGLCVNTDPVSILTSILDMSLHRQMGSDRDLQSSASSVSLPSVKKAPKKRRISIGSL FRRKKDNKRKSRELNGGVDGIASIESIHSEMCTDKNSIFSTNTSSDNGLTSISKQIGDFIECPLCLLRHS KDRFPDIMTCHHRSCVDCLRQYLRIEISESRVNISCPECTERFNPHDIRLILSDDVLMEKYEEFMLRRWL VADPDCRWCPAPDCGYAVIAFGCASCPKLTCGREGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTI RSSSISYSQESGAAADDIKPCPRCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFW GKKPWSRKKKILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHNRYEGKDVSKHKRNLAIAGGV TLSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRSGGCGVSAGNGKGVRIEFDDENDINVGGTNTAVD TTSVAEARHNPSIGEGSVGGLTGSLSASGSHMDRIGAIRDNLSETASTMALAGASITGSLSGSAMVNCFN RLEVQADVQKERYSLSGESGTVSLGTVSDNASTKAMAGSILNSYIPLDKEGNSMEVQVDIESKPSKFRHN SGSSSVDDGSATRSHAGGSSSGLPEGKSSATKWSKEATAGKKSKSGKLRKKGNMKINETREDMDAQLLEQ QSTNSSEFEAPSLSDSMPSVADSHSSHFSEFSCSDLESMKTSCSHGSSDYHTRFATVNILPEVENDRLEN SPHQCSISVVTQTASCSEVSQLNHIAEEHGNNGIKPNVDLYFGDALKETNNNHSHQTMELKVAIQTEI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015435 |
| ORF Size | 2514 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015435.4 |
| RefSeq Size | 4357 bp |
| RefSeq ORF | 2517 bp |
| Locus ID | 25897 |
| UniProt ID | Q9NV58 |
| Domains | RING, IBR |
| Protein Families | Druggable Genome, Transcription Factors, Transmembrane |
| Gene Summary | This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222087 | RNF19A (Myc-DDK-tagged)-Human ring finger protein 19A (RNF19A), transcript variant 2 |
CNY 3,990.00 |
|
| RC222087L3 | Lenti ORF clone of Human ring finger protein 19A (RNF19A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222087L4 | Lenti ORF clone of Human ring finger protein 19A (RNF19A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC108420 | RNF19A (untagged)-Human ring finger protein 19A (RNF19A), transcript variant 2 |
CNY 6,144.00 |
