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GPBAR1 (NM_170699) Human Tagged ORF Clone
CAT＃: RG221897
- TrueORF®
GPBAR1 (tGFP-tagged) - Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3
ORF Plasmid: DDK
"NM_170699" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BG37; GPCR19; GPR131; M-BAR; TGR5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221897 representing NM_170699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTTGGTCCTTGTGCTCTTCAGCCAGGACACCAGACATGGTCCAAACCGCTGCAGGGCTGGCTGCA GCAACTCCCTGACACTCAGGAAGGCCCAGGCTGGGCAGGCAATACCTGCTCCCAACAGCCATGCATGCCG GCTGCCGCTCCAGGACTCCCCTGTCCCCAGGACCAAGATGACGCCCAACAGCACTGGCGAGGTGCCCAGC CCCATTCCCAAGGGGGCTTTGGGGCTCTCCCTGGCCCTGGCAAGCCTCATCATCACCGCGAACCTGCTCC TAGCCCTGGGCATCGCCTGGGACCGCCGCCTGCGCAGCCCACCTGCTGGCTGCTTCTTCCTGAGCCTACT GCTGGCTGGGCTGCTCACGGGTCTGGCATTGCCCACATTGCCAGGGCTGTGGAACCAGAGTCGCCGGGGT TACTGGTCCTGCCTCCTCGTCTACTTGGCTCCCAACTTCTCCTTCCTCTCCCTGCTTGCCAACCTCTTGC TGGTGCACGGGGAGCGCTACATGGCAGTCCTGAGGCCACTCCAGCCCCCTGGGAGCATTCGGCTGGCCCT GCTCCTCACCTGGGCTGGTCCCCTGCTCTTTGCCAGTCTGCCCGCTCTGGGGTGGAACCACTGGACCCCT GGTGCCAACTGCAGCTCCCAGGCTATCTTCCCAGCCCCCTACCTGTACCTCGAAGTCTATGGGCTCCTGC TGCCCGCCGTGGGTGCTGCTGCCTTCCTCTCTGTCCGCGTGCTGGCCACTGCCCACCGCCAGCTGCAGGA CATCTGCCGGCTGGAGCGGGCAGTGTGCCGCGATGAGCCCTCCGCCCTGGCCCGGGCCCTTACCTGGAGG CAGGCAAGGGCACAGGCTGGAGCCATGCTGCTCTTCGGGCTGTGCTGGGGGCCCTACGTGGCCACACTGC TCCTCTCAGTCCTGGCCTATGAGCAGCGCCCGCCACTGGGGCCTGGGACACTGTTGTCCCTCCTCTCCCT AGGAAGTGCCAGTGCAGCGGCAGTGCCCGTAGCCATGGGGCTGGGCGATCAGCGCTACACAGCCCCCTGG AGGGCAGCCGCCCAAAGGTGCCTGCAGGGGCTGTGGGGAAGAGCCTCCCGGGACAGTCCCGGCCCCAGCA TTGCCTACCACCCAAGCAGCCAAAGCAGTGTCGACCTGGACTTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221897 representing NM_170699
Red=Cloning site Green=Tags(s) MHLVLVLFSQDTRHGPNRCRAGCSNSLTLRKAQAGQAIPAPNSHACRLPLQDSPVPRTKMTPNSTGEVPS PIPKGALGLSLALASLIITANLLLALGIAWDRRLRSPPAGCFFLSLLLAGLLTGLALPTLPGLWNQSRRG YWSCLLVYLAPNFSFLSLLANLLLVHGERYMAVLRPLQPPGSIRLALLLTWAGPLLFASLPALGWNHWTP GANCSSQAIFPAPYLYLEVYGLLLPAVGAAAFLSVRVLATAHRQLQDICRLERAVCRDEPSALARALTWR QARAQAGAMLLFGLCWGPYVATLLLSVLAYEQRPPLGPGTLLSLLSLGSASAAAVPVAMGLGDQRYTAPW RAAAQRCLQGLWGRASRDSPGPSIAYHPSSQSSVDLDLN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_170699 |
| ORF Size | 1167 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170699.1, NP_733800.1 |
| RefSeq Size | 1400 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 151306 |
| UniProt ID | Q8TDU6 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221897 | GPBAR1 (Myc-DDK-tagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3 |
CNY 2,400.00 |
|
| RC221897L1 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221897L2 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC221897L3 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221897L4 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| SC123312 | GPBAR1 (untagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3 |
CNY 2,400.00 |
