TBX5 (NM_181486) Human Tagged ORF Clone
CAT#: RG221808
- TrueORF®
TBX5 (tGFP-tagged) - Human T-box 5 (TBX5), transcript variant 4
ORF Plasmid: DDK
"NM_181486" in other vectors (4)
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CNY 5,456.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HOS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221808 representing NM_181486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCCTGACGCAAAAGACCTGCCCT GCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCGTCCCCGCAGGCCGCCTTCAC CCAGCAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTG GGCACGGAAATGATCATAACCAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCC TTAATCCCAAAACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGC AGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGAC TCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACC ACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAAATACCAGCCTAGATTACACATCGTGAA AGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTTCCTGAGACTGCG TTTATAGCAGTGACTTCCTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCA AAGGATTTCGGGGCAGTGATGACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGT GGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTCTCGAGCT CTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACC TCCTGCCTCCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAA AGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAGCCCTACATGGAGACATCACCCAGTGAAGAA GATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCAC AGCGGCAAGCTTGCATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAG CTGCAACACGTGGCCAAGCATGCCTTCCTACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGG CTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCTGGCATGGCCA ACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGT CAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTCCCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCT CATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCACGGAGTTGGCATGGTGCCAGAGT GGAGCGACAATAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221808 representing NM_181486
Red=Cloning site Green=Tags(s) MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEV GTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPD SPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETA FIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRA LSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEE DSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDR LPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYS HGVPRTLSPHQYHSVHGVGMVPEWSDNS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181486 |
ORF Size | 1554 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181486.4 |
RefSeq Size | 3749 bp |
RefSeq ORF | 1557 bp |
Locus ID | 6910 |
UniProt ID | Q99593 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221808 | TBX5 (Myc-DDK-tagged)-Human T-box 5 (TBX5), transcript variant 4 |
CNY 3,856.00 |
|
RC221808L3 | Lenti ORF clone of Human T-box 5 (TBX5), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC221808L4 | Lenti ORF clone of Human T-box 5 (TBX5), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
SC124969 | TBX5 (untagged)-Human T-box 5 (TBX5), transcript variant 4 |
CNY 7,220.00 |