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AKAP4 (NM_003886) Human Tagged ORF Clone
CAT＃: RG221765
- TrueORF®
AKAP4 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1
ORF Plasmid: DDK
"NM_003886" in other vectors (4)
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CNY 10,984.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AKAP-4; AKAP 82; AKAP82; CT99; FSC1; hAKAP82; HI; p82; PRKA4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221765 representing NM_003886
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGTACTCTGATACTACAATGATGTCTGATGATATTGACTGGTTACGCAGCCACAGGGGTGTGT GCAAGGTAGATCTCTACAACCCAGAAGGACAGCAAGATCAGGACCGGAAAGTGATATGCTTTGTCGATGT GTCCACCCTGAATGTAGAAGATAAAGATTACAAGGATGCTGCTAGTTCCAGCTCAGAAGGCAACTTAAAC CTGGGAAGTCTGGAAGAAAAAGAGATTATCGTGATCAAGGACACTGAGAAGAAAGACCAGTCTAAGACAG AGGGATCTGTATGCCTTTTCAAACAAGCTCCCTCTGATCCTGTAAGTGTCCTCAACTGGCTTCTCAGTGA TCTCCAGAAGTATGCCTTGGGTTTCCAACATGCACTGAGCCCCTCAACCTCTACCTGTAAACATAAAGTA GGAGACACAGAGGGCGAATATCACAGAGCATCCTCTGAGAACTGCTACAGTGTCTATGCCGATCAAGTGA ACATAGATTATTTGATGAACAGACCTCAAAACCTACGTCTAGAAATGACAGCAGCTAAAAACACCAACAA TAATCAAAGTCCTTCAGCTCCTCCAGCCAAACCTCCTAGCACTCAGAGAGCAGTCATTTCCCCTGATGGA GAATGTTCTATAGATGACCTTTCCTTCTACGTCAACCGACTATCTTCTCTGGTAATCCAGATGGCCCATA AGGAAATCAAGGAGAAGTTGGAAGGTAAAAGCAAATGCCTTCATCATTCAATCTGTCCATCCCCTGGGAA CAAAGAGAGAATCAGTCCCCGAACTCCTGCGAGCAAGATTGCTTCTGAAATGGCCTATGAAGCTGTGGAA CTGACAGCTGCAGAAATGCGTGGCACTGGAGAGGAGTCCAGGGAAGGTGGCCAGAAAAGCTTTCTATATA GCGAATTATCCAACAAGAGCAAAAGTGGAGACAAACAGATGTCCCAGAGAGAGAGCAAAGAATTTGCAGA TTCCATCAGCAAGGGGCTCATGGTTTATGCAAATCAGGTGGCATCTGACATGATGGTCTCTCTCATGAAG ACCTTGAAAGTGCACAGCTCTGGGAAGCCAATTCCAGCATCTGTGGTCCTGAAGAGGGTGTTGCTAAGGC ACACCAAGGAGATTGTGTCCGATTTGATTGATTCTTGCATGAAGAACCTGCATAATATTACTGGGGTCCT GATGACTGACTCAGACTTTGTCTCAGCTGTCAAGAGAAATCTGTTCAACCAGTGGAAACAAAATGCTACA GACATCATGGAGGCCATGCTGAAGCGCTTGGTCAGTGCCCTTATAGGTGAGGAGAAGGAGACTAAGTCTC AGAGTCTGTCATATGCATCTTTAAAAGCTGGGTCCCATGATCCCAAATGCAGGAATCAGAGTCTTGAATT CTCCACCATGAAAGCTGAAATGAAAGAGAGGGACAAAGGCAAAATGAAATCAGACCCATGCAAGTCACTG ACTAGTGCTGAGAAAGTCGGTGAACACATTCTCAAAGAGGGCCTAACCATCTGGAACCAAAAGCAAGGAA ACTCATGCAAGGTGGCTACCAAAGCATGCAGCAATAAAGATGAGAAAGGAGAAAAGATCAATGCTTCCAC AGATTCACTGGCCAAGGACCTGATTGTCTCTGCCCTTAAGCTGATCCAGTACCATCTGACCCAGCAGACT AAGGGCAAAGATACATGTGAAGAAGACTGTCCTGGTTCCACCATGGGCTATATGGCTCAGAGTACTCAAT ATGAAAAGTGTGGAGGTGGCCAAAGTGCCAAAGCACTTTCAGTGAAACAACTAGAATCTCACAGAGCCCC TGGACCATCCACCTGTCAAAAGGAGAACCAACACCTGGACTCCCAGAAAATGGATATGTCAAACATCGTT CTAATGCTGATTCAGAAACTGCTTAATGAGAACCCCTTCAAATGTGAGGATCCATGCGAAGGTGAGAACA AGTGTTCTGAGCCCAGGGCAAGCAAAGCAGCTTCCATGTCCAACAGATCTGACAAAGCGGAAGAACAATG CCAGGAGCATCAAGAACTTGACTGTACCAGTGGGATGAAGCAAGCGAACGGGCAATTTATAGATAAACTA GTAGAATCTGTGATGAAGCTCTGCCTTATCATGGCTAAGTATAGCAACGATGGGGCAGCCCTTGCTGAGT TGGAAGAACAAGCAGCCTCGGCAAATAAGCCCAATTTCAGGGGCACCAGATGCATTCACAGTGGTGCAAT GCCACAGAACTATCAAGACTCTCTTGGACATGAAGTAATTGTCAATAATCAGTGCTCTACAAATAGCTTG CAGAAGCAGCTCCAGGCTGTCCTGCAGTGGATTGCAGCCTCCCAGTTTAACGTGCCCATGCTCTACTTCA TGGGAGATAAGGATGGACAACTGGAAAAGCTTCCTCAGGTTTCAGCTAAAGCAGCAGAGAAGGGGTACAG TGTAGGAGGTCTTCTTCAAGAGGTCATGAAGTTTGCCAAGGAACGGCAACCAGATGAAGCTGTGGGAAAG GTGGCCAGGAAACAGTTGCTGGACTGGCTGCTCGCTAACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221765 representing NM_003886
Red=Cloning site Green=Tags(s) MMAYSDTTMMSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLN LGSLEEKEIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKV GDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDG ECSIDDLSFYVNRLSSLVIQMAHKEIKEKLEGKSKCLHHSICPSPGNKERISPRTPASKIASEMAYEAVE LTAAEMRGTGEESREGGQKSFLYSELSNKSKSGDKQMSQRESKEFADSISKGLMVYANQVASDMMVSLMK TLKVHSSGKPIPASVVLKRVLLRHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNQWKQNAT DIMEAMLKRLVSALIGEEKETKSQSLSYASLKAGSHDPKCRNQSLEFSTMKAEMKERDKGKMKSDPCKSL TSAEKVGEHILKEGLTIWNQKQGNSCKVATKACSNKDEKGEKINASTDSLAKDLIVSALKLIQYHLTQQT KGKDTCEEDCPGSTMGYMAQSTQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIV LMLIQKLLNENPFKCEDPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQANGQFIDKL VESVMKLCLIMAKYSNDGAALAELEEQAASANKPNFRGTRCIHSGAMPQNYQDSLGHEVIVNNQCSTNSL QKQLQAVLQWIAASQFNVPMLYFMGDKDGQLEKLPQVSAKAAEKGYSVGGLLQEVMKFAKERQPDEAVGK VARKQLLDWLLANL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003886 |
| ORF Size | 2562 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003886.3 |
| RefSeq Size | 2881 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 8852 |
| UniProt ID | Q5JQC9 |
| Protein Families | Druggable Genome |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A-Kinase Anchoring Protein 4 (AKAP4) is an ERK1/2 substrate and a switch molecule between cAMP/PKA and PKC/ERK1/2 in human spermatozoa
,Rahamim Ben-Navi, L;Almog, T;Yao, Z;Seger, R;Naor, Z;,
Sci Rep
,PubMed ID 27901058
[AKAP4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221765 | AKAP4 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1 |
CNY 9,384.00 |
|
| RC221765L3 | Lenti-ORF clone of AKAP4 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1 |
CNY 5,890.00 |
|
| RC221765L4 | Lenti-ORF clone of AKAP4 (mGFP-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1 |
CNY 5,890.00 |
|
| SC303398 | AKAP4 (untagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1 |
CNY 7,220.00 |
