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SPFH2 (ERLIN2) (NM_007175) Human Tagged ORF Clone
CAT＃: RG221700
- TrueORF®
ERLIN2 (tGFP-tagged) - Human ER lipid raft associated 2 (ERLIN2), transcript variant 1
ORF Plasmid: DDK
"NM_007175" in other vectors (6)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C8orf2; Erlin-2; NET32; SPFH2; SPG18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221700 representing NM_007175
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTGGGAGCAGTTGTGGCTGTGGCTTCCAGTTTCTTTTGTGCATCTCTCTTCTCAGCTGTGC ACAAGATAGAAGAGGGACATATTGGGGTATATTACAGAGGCGGTGCCCTGCTGACTTCGACCAGCGGCCC TGGTTTCCATCTCATGCTCCCTTTCATCACATCATATAAGTCTGTGCAGACCACACTCCAGACAGATGAG GTGAAGAATGTACCTTGTGGGACTAGTGGTGGTGTGATGATCTACTTTGACAGAATTGAAGTGGTGAACT TCCTGGTCCCGAACGCAGTGTATGATATAGTGAAGAACTATACTGCTGACTATGACAAGGCCCTCATCTT CAACAAGATCCACCACGAACTGAACCAGTTCTGCAGTGTGCACACGCTTCAAGAGGTCTACATTGAGCTG TTTGATCAGATTGATGAAAATCTCAAACTGGCTTTGCAACAGGACCTGACCTCCATGGCCCCTGGGCTGG TCATTCAAGCTGTGCGGGTAACAAAGCCCAACATACCAGAGGCAATCCGCAGAAACTACGAGTTGATGGA AAGTGAGAAGACAAAGCTTCTCATTGCCGCCCAGAAACAGAAGGTGGTGGAAAAGGAAGCAGAGACAGAG CGGAAGAAGGCGCTCATTGAGGCAGAAAAAGTGGCCCAGGTGGCTGAGATCACCTACGGGCAGAAGGTGA TGGAGAAGGAGACTGAGAAGAAGATTTCAGAAATTGAAGATGCTGCATTTCTGGCCCGGGAGAAGGCAAA GGCAGATGCTGAGTGCTACACTGCTATGAAAATAGCCGAAGCCAATAAGCTGAAGCTAACCCCTGAATAT CTGCAGCTGATGAAGTACAAGGCCATTGCTTCCAACAGCAAGATTTACTTTGGCAAAGACATTCCTAACA TGTTCATGGACTCTGCGGGCAGTGTGAGCAAGCAGTTTGAGGGGCTAGCTGACAAGCTAAGCTTTGGCTT AGAAGATGAACCCTTGGAGACGGCCACTAAGGAGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221700 representing NM_007175
Red=Cloning site Green=Tags(s) MAQLGAVVAVASSFFCASLFSAVHKIEEGHIGVYYRGGALLTSTSGPGFHLMLPFITSYKSVQTTLQTDE VKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIEL FDQIDENLKLALQQDLTSMAPGLVIQAVRVTKPNIPEAIRRNYELMESEKTKLLIAAQKQKVVEKEAETE RKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLAREKAKADAECYTAMKIAEANKLKLTPEY LQLMKYKAIASNSKIYFGKDIPNMFMDSAGSVSKQFEGLADKLSFGLEDEPLETATKEN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007175 |
| ORF Size | 1017 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007175.8 |
| RefSeq Size | 4836 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 11160 |
| UniProt ID | O94905 |
| Domains | Band_7 |
| Gene Summary | This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Discovering novel pharmacogenomic biomarkers by imputing drug response in cancer patients from large genomics studies
,null,
Genome Research
,PubMed ID 28847918
[ERLIN2]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221700 | ERLIN2 (Myc-DDK-tagged)-Human ER lipid raft associated 2 (ERLIN2), transcript variant 1 |
CNY 5,488.00 |
|
| RC221700L1 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221700L2 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC221700L3 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221700L4 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| SC115663 | ERLIN2 (untagged)-Human ER lipid raft associated 2 (ERLIN2), transcript variant 1 |
CNY 3,656.00 |
