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CHD1 (NM_001270) Human Tagged ORF Clone
CAT＃: RG221575
- TrueORF®
CHD1 (tGFP-tagged) - Human chromodomain helicase DNA binding protein 1 (CHD1)
ORF Plasmid: DDK
"NM_001270" in other vectors (4)
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CNY 15,200.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CHD-1; PILBOS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221575 representing NM_001270.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATGGACACAGTGATGAAGAAAGTGTTAGAAACAGTAGTGGAGAATCAAGCCAGTCGGATGATGAT TCTGGGTCAGCTTCAGGCTCTGGATCTGGTTCGAGTTCTGGAAGCAGTAGTGATGGAAGCAGTAGCCAG TCAGGTAGCAGTGACTCTGACTCCGGATCTGAATCAGGCAGTCAGTCAGAGTCTGAGTCAGACACTTCC CGAGAAAACAAAGTTCAAGCAAAACCACCGAAAGTTGATGGAGCTGAGTTTTGGAAATCTAGTCCTAGT ATTCTGGCCGTTCAGAGATCTGCAATCCTCAAGAAGCAGCAACAGCAGCAGCAGCAACAACAACATCAA GCCTCATCTAATAGCGGATCAGAAGAGGATTCCTCTAGCAGTGAAGATTCCGATGACTCATCAAGTGAG GTCAAAAGGAAAAAGCATAAAGATGAAGATTGGCAAATGTCTGGGTCAGGATCTCCATCTCAGTCTGGT TCAGATTCAGAATCTGAAGAAGAGAGAGAGAAAAGCAGTTGTGATGAAACAGAATCTGATTATGAGCCA AAAAACAAAGTCAAAAGCAGAAAACCTCAAAATAGATCTAAGTCAAAAAATGGAAAGAAGATTCTTGGA CAAAAAAAGAGACAGATTGATTCATCTGAGGAGGATGATGATGAAGAAGATTATGATAATGATAAAAGA AGTTCTCGTCGCCAAGCAACTGTTAATGTTAGCTATAAGGAGGATGAAGAAATGAAAACAGATTCTGAT GACCTACTGGAAGTCTGTGGAGAGGATGTTCCTCAACCTGAGGAAGAGGAATTTGAAACCATAGAAAGA TTTATGGATTGTCGGATTGGGAGAAAAGGAGCTACTGGTGCTACTACAACCATCTATGCAGTTGAAGCA GATGGTGACCCAAATGCAGGCTTTGAAAAAAACAAAGAACCAGGAGAGATTCAGTATTTAATTAAATGG AAAGGATGGTCCCATATCCACAACACTTGGGAGACAGAAGAAACCCTCAAGCAGCAGAATGTTAGAGGA ATGAAAAAATTGGATAATTATAAGAAAAAAGATCAGGAAACAAAAAGATGGTTGAAAAATGCCTCTCCA GAAGATGTGGAATATTATAATTGCCAGCAAGAACTTACAGATGATCTACATAAACAGTATCAAATAGTG GAACGTATAATTGCTCATTCCAATCAAAAGTCAGCAGCTGGTTATCCTGATTATTACTGCAAATGGCAG GGCCTTCCATACTCAGAGTGCAGCTGGGAAGATGGAGCTCTCATTTCCAAAAAGTTTCAAGCATGCATT GATGAGTATTTTAGCAGGAACCAATCAAAAACCACTCCTTTTAAAGATTGCAAAGTATTAAAACAAAGG CCAAGGTTTGTAGCCCTGAAGAAGCAGCCATCCTATATTGGAGGACATGAGGGCTTAGAATTAAGAGAT TATCAACTGAATGGTTTAAATTGGCTTGCTCATTCTTGGTGCAAAGGAAATAGTTGCATACTCGCTGAT GAAATGGGCCTTGGAAAAACAATACAGACGATCTCATTTCTGAATTATTTGTTTCATGAACATCAATTA TATGGACCTTTTTTATTGGTAGTACCGCTCTCCACTCTTACTTCCTGGCAAAGGGAAATTCAGACTTGG GCTTCTCAAATGAATGCTGTGGTTTATTTAGGTGACATTAACAGCAGAAACATGATAAGAACTCATGAA TGGACGCATCATCAGACCAAACGGTTAAAATTTAATATATTGTTAACAACTTATGAAATTTTATTAAAA GATAAGGCATTCCTTGGAGGTCTAAATTGGGCATTTATAGGTGTTGATGAAGCACACCGATTAAAGAAT GATGACTCCCTTCTGTATAAAACTTTAATAGATTTTAAATCCAATCATCGTCTCCTTATCACTGGAACT CCTCTACAGAATTCCCTCAAAGAGCTCTGGTCTTTGCTACATTTCATTATGCCAGAAAAGTTTTCTTCC TGGGAAGATTTTGAAGAAGAACATGGCAAAGGGAGAGAATATGGTTATGCAAGCCTTCACAAGGAGCTT GAGCCATTTCTGTTACGCCGAGTTAAGAAAGATGTGGAAAAATCTCTTCCTGCCAAGGTTGAGCAGATT TTAAGAATGGAAATGAGTGCTTTACAGAAACAATATTACAAATGGATTTTAACTAGGAATTACAAAGCC CTCAGCAAAGGTTCCAAGGGCAGTACCTCAGGCTTTTTGAACATTATGATGGAGCTAAAGAAATGTTGT AACCATTGCTACCTCATTAAACCACCAGATAATAATGAATTCTATAATAAACAGGAGGCCTTACAACAC TTAATTCGTAGTAGCGGAAAATTGATTCTTCTTGACAAGCTATTAATTCGCCTAAGAGAACGAGGCAAT CGAGTTCTTATTTTTTCACAAATGGTGCGGATGTTAGATATACTTGCAGAATATTTGAAATATCGTCAA TTCCCCTTTCAAAGATTAGATGGATCAATAAAAGGAGAACTGAGGAAACAAGCTCTAGATCATTTTAAT GCTGAGGGATCAGAGGATTTTTGCTTTTTGCTGTCCACAAGAGCTGGAGGTCTAGGGATTAATTTAGCC TCTGCTGACACTGTTGTTATATTTGATTCCGATTGGAATCCACAGAATGATCTTCAGGCACAGGCTAGA GCCCATCGAATTGGGCAAAAGAAACAGGTGAATATTTATCGTCTAGTTACAAAGGGATCAGTTGAAGAA GATATTCTTGAAAGGGCGAAAAAGAAGATGGTTTTAGATCATCTTGTAATTCAAAGAATGGACACAACT GGGAAGACAGTACTACATACAGGTTCTGCCCCATCAAGTTCTACTCCTTTCAATAAAGAAGAGTTATCA GCCATTTTAAAGTTTGGTGCTGAAGAACTTTTTAAGGAACCTGAAGGAGAAGAACAAGAGCCCCAGGAA ATGGATATAGATGAAATCTTGAAGAGAGCTGAAACTCATGAAAATGAACCAGGTCCTTTAACTGTAGGA GATGAATTGCTTTCCCAGTTCAAGGTTGCCAACTTCTCAAATATGGATGAGGATGACATTGAGTTGGAA CCTGAAAGAAATTCAAAGAATTGGGAGGAAATTATTCCAGAAGATCAAAGAAGACGATTAGAAGAAGAA GAAAGACAAAAGGAACTTGAAGAAATTTATATGCTCCCAAGAATGAGAAATTGTGCAAAACAGATTAGT TTCAATGGAAGTGAAGGGAGGCGCAGTAGAAGTAGGAGATACTCTGGATCTGATAGTGATTCCATCTCA GAAGGGAAAAGGCCAAAGAAACGTGGAAGACCACGGACTATTCCTCGGGAGAATATTAAAGGATTTAGT GATGCAGAAATTAGGCGGTTTATCAAGAGCTATAAGAAATTTGGTGGTCCTCTGGAAAGATTAGATGCA ATTGCTCGAGATGCTGAGTTAGTTGATAAGTCAGAAACAGACCTTAGACGACTGGGAGAATTGGTACAT AATGGTTGCATTAAAGCATTAAAGGATAGTTCTTCAGGAACAGAACGAACAGGTGGTAGACTCGGAAAA GTGAAGGGTCCAACATTCCGAATATCAGGAGTACAGGTGAATGCCAAACTAGTCATCTCCCATGAAGAA GAATTAATACCTTTGCACAAATCCATTCCTTCTGATCCAGAAGAAAGAAAGCAGTATACTATCCCATGC CACACAAAGGCAGCTCATTTTGATATAGACTGGGGCAAAGAAGATGATTCCAATTTGTTAATTGGCATC TATGAATATGGATATGGAAGCTGGGAAATGATTAAAATGGATCCTGACCTCAGTCTAACACACAAGATT CTTCCAGATGATCCCGATAAAAAACCACAAGCAAAACAGTTGCAGACCCGTGCAGACTACCTCATCAAA TTACTTAGTAGAGATCTTGCAAAAAAAGAAGCTCTTTCTGGTGCGGGAAGTTCAAAGAGGAGAAAAGCA AGAGCTAAGAAGAATAAAGCAATGAAGTCTATAAAAGTGAAAGAGGAAATAAAGAGTGATTCTTCTCCT CTGCCTTCAGAGAAGTCTGATGAAGATGATGATAAATTGAGTGAATCCAAGTCTGATGGTAGGGAAAGA TCCAAGAAATCTTCAGTGTCAGATGCTCCAGTTCATATCACGGCAAGTGGTGAACCAGTTCCCATTTCT GAAGAATCTGAAGAGCTGGATCAGAAGACATTCAGCATTTGTAAAGAAAGAATGAGGCCTGTTAAAGCA GCTTTGAAACAACTTGATAGGCCTGAGAAAGGCCTTTCAGAAAGAGAACAACTAGAACATACTAGACAA TGTTTAATAAAAATTGGAGACCATATCACAGAATGTCTAAAAGAGTATACAAATCCTGAACAAATTAAG CAATGGAGAAAAAACCTGTGGATTTTTGTATCTAAGTTTACTGAATTTGATGCAAGAAAATTACATAAA TTATATAAGCATGCTATTAAAAAACGGCAGGAGTCTCAGCAAAACAGTGATCAAAACAGCAACTTGAAT CCTCACGTGATTAGAAATCCAGATGTGGAAAGATTAAAAGAGAATACAAATCACGATGATAGCAGCAGG GACAGTTATTCCTCTGATAGACACTTAACTCAGTACCATGATCATCATAAAGACCGACATCAGGGAGAT TCTTACAAAAAAAGTGATTCCAGGAAAAGACCCTATTCTTCTTTTAGTAATGGTAAAGACCATCGTGAT TGGGATCACTACAAGCAAGACAGCAGATATTACAGTGACAGAGAGAAACACAGAAAACTGGATGATCAC AGGAGTAGAGATCACAGGTCAAATTTGGAAGGAAGTTTAAAAGATAGATCTCATTCTGATCATCGTTCT CACTCAGATCATCGGTTACATTCAGACCACCGGTCAAGTTCTGAATATACGCACCATAAATCTTCCAGG GATTATAGGTATCACTCAGACTGGCAAATGGACCACAGAGCTTCCAGCAGTGGCCCTAGGTCACCACTA GATCAGAGATCTTATGGCTCCAGATCTCCATTTGAACATTCAGTTGAACACAAAAGTACACCGGAGCAT ACCTGGAGTAGTCGGAAAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG221575
Blue=ORF Red=Cloning site Green=Tag(s) MNGHSDEESVRNSSGESSQSDDDSGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSESGSQSESESDTS RENKVQAKPPKVDGAEFWKSSPSILAVQRSAILKKQQQQQQQQQHQASSNSGSEEDSSSSEDSDDSSSE VKRKKHKDEDWQMSGSGSPSQSGSDSESEEEREKSSCDETESDYEPKNKVKSRKPQNRSKSKNGKKILG QKKRQIDSSEEDDDEEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEEEEFETIER FMDCRIGRKGATGATTTIYAVEADGDPNAGFEKNKEPGEIQYLIKWKGWSHIHNTWETEETLKQQNVRG MKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGYPDYYCKWQ GLPYSECSWEDGALISKKFQACIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRD YQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTW ASQMNAVVYLGDINSRNMIRTHEWTHHQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKN DDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKEL EPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCC NHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQ FPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQAR AHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELS AILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELE PERNSKNWEEIIPEDQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSIS EGKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVH NGCIKALKDSSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDPEERKQYTIPC HTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIK LLSRDLAKKEALSGAGSSKRRKARAKKNKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLSESKSDGRER SKKSSVSDAPVHITASGEPVPISEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQ CLIKIGDHITECLKEYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNLN PHVIRNPDVERLKENTNHDDSSRDSYSSDRHLTQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRD WDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRSHSDHRSHSDHRLHSDHRSSSEYTHHKSSR DYRYHSDWQMDHRASSSGPRSPLDQRSYGSRSPFEHSVEHKSTPEHTWSSRKT TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270 |
| ORF Size | 5127 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6457 bp |
| RefSeq ORF | 5133 bp |
| Locus ID | 1105 |
| UniProt ID | O14646 |
| Domains | SNF2_N, CHROMO, DEAD, helicase_C |
| Protein Families | Druggable Genome |
| MW | 196.6 kDa |
| Gene Summary | The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221575 | CHD1 (Myc-DDK-tagged)-Human chromodomain helicase DNA binding protein 1 (CHD1) |
CNY 17,232.00 |
|
| RC221575L1 | Lenti ORF clone of Human chromodomain helicase DNA binding protein 1 (CHD1), Myc-DDK-tagged |
CNY 15,680.00 |
|
| RC221575L3 | Lenti ORF clone of Human chromodomain helicase DNA binding protein 1 (CHD1), Myc-DDK-tagged |
CNY 15,680.00 |
|
| SC309044 | CHD1 (untagged)-Human chromodomain helicase DNA binding protein 1 (CHD1) |
CNY 11,504.00 |
