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DACH1 (NM_080759) Human Tagged ORF Clone
CAT＃: RG221505
- TrueORF®
DACH1 (tGFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1
ORF Plasmid: DDK
"NM_080759" in other vectors (7)
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CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DACH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221505 representing NM_080759
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACG GAGGCGGCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAG CGGCGGCGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACC GGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCA GCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAA TAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTG ATCTGCCTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCA AGCTGAAGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGG CGCCATCCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGAC TGCACCAACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACT CTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGC AGCCGCTGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAA TTAGAAGCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGA ATTCAAGTGTCGGACTGGAACTTCCTTTTATGATGATGCCCCACCCTCTAATTCCTGTCAGCCTACCTCC AGCATCTGTCACCATGGCAATGAGCCAGATGAACCACCTCAGCACCATTGCAAATATGGCAGCAGCAGCA CAAGTTCAGAGTCCCCCATCCAGAGTTGAGACATCAGTTATTAAGGAGCGTGTTCCTGATAGCCCCTCAC CTGCCCCCTCTCTGGAGGAGGGGAGAAGGCCTGGCAGTCACCCATCATCACATCGCAGCAGCAGCGTGTC CAGCTCCCCTGCTCGGACTGAGAGCTCTTCTGACAGAATCCCGGTCCATCAGAATGGGTTGTCCATGAAC CAGATGCTGATGGGCTTATCACCAAATGTACTTCCTGGGCCCAAAGAGGGAGATTTGGCCGGTCATGACA TGGGACATGAGTCAAAAAGGATGCATATTGAAAAAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGA CAGCCTTGACAAACTCTCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTG TTTCCTGATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAG ATAATGCCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGA AAGAGAACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAG AGGCTAAAGAAGGAGAAGAAGGCAAAGAGAAAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTG AACAAGCAGAACAGACGCTAAAACAGGCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGAC CCCAGAGATAGAGGCTGACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTG TATTTGAAAACTACTGTCATGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221505 representing NM_080759
Red=Cloning site Green=Tags(s) MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATV TSTGGGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINAST GSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCEL ICLPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYND CTNASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIK LEAMSNYHASNNQHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAA QVQSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMN QMLMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFL FPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQK RLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRL YLKTTVMY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080759 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080759.4 |
| RefSeq Size | 5240 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 1602 |
| UniProt ID | Q9UI36 |
| Domains | Ski_Sno |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221505 | DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 |
CNY 7,410.00 |
|
| RC221505L1 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged |
CNY 10,672.00 |
|
| RC221505L2 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged |
CNY 9,310.00 |
|
| RC221505L3 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged |
CNY 9,310.00 |
|
| RC221505L4 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged |
CNY 9,310.00 |
|
| SC120348 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 |
CNY 7,768.00 |
|
| SC327980 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 1 |
CNY 13,680.00 |
